Further studies of the physical and metabolic properties of foot-and-mouth disease virus temperature-sensitive mutants

Abstract

Three temperature-sensitive (ts) mutants of foot-and-mouth disease virus were classified as ribonucleic acid negative and as belonging to the same complementation group when measured by virus yields and [:H]uridine incorporation in paired, mixed infections at the nonpermissive temperature (38.5 C). Mutant ts-22, the only mutant able to produce plaques at 38.5 C, was more sensitive to acid than were the parental wild-type or other mutant viruses. Diethylaminoethyl-dextran did not enhance the plaque-forming ability of the mutant viruses at 38.5 C. All of the viruses inhibited host cell protein synthesis at both permissive (33 C) and nonpermissive (38.5 C) temperatures.

Evidence for a mouse pathogenicity locus in certain temperature-sensitive mutants of foot-and-mouth disease virus

“…Defining complementation groups for ts mutants of FMD viruses (13,22) has been difficult. Both virus recovery and isotope incorporation procedures were used to establish that these three mutants belonged to the same complementation group (24). Two of these mutants exhibited reduced virulence for infant mice; they were immunogenic and induced resistance to subsequent infection by the parental virus, but not to a heterologous type of FMD virus (23).…”