Further Studies in Changes of Nuclear Population in Degnerating Non-Myelinated and Finely Myelinated Nerves

Joseph, JE

doi:10.1159/000140442

Cited by 28 publications

(6 citation statements)

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“…In earlier studies, it was observed that during crush injury of peripheral nerves, there was a substantial increase (up to eightfold) in the number of tubal nuclei (Schwann cell nuclei) distal to the site of injury after 25 d, which then declined slightly (11). This increase in cell number was found to be greatest in myelinated nerves and least in thinly myelinated or in unmyelinated nerves (58,38) . More recent autoradiographic studies have shown that the increase in labeling that occurs during Wallerian degeneration is greater in sciatic nerve (11,27) than in the unmyelinated cervical sympathetic trunk (49).…”

Section: Proliferation Of Celts During Degenerationmentioning

confidence: 96%

Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury.

Salzer¹,

Bunge²

1980

The Journal of Cell Biology

393

213

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In this paper the stimuli for and pattern of Schwann cell proliferation are defined under various experimental conditions . We used a tissue culture system in which fetal rat dorsal root ganglia, treated to eliminate contaminating fibroblasts (Wood, P ., 1976, Brain Res. 115 :361-375), appear to recapitulate many aspects of the developing peripheral nervous system . We observed that : (a) proliferation of Schwann cells on neurites is initially rapid, but, as each neurite becomes fully KEY WORDS Schwann cell proliferation " nerve tissue culture " autoradiography development . degeneration injury Peripheral nerve development is an ordered process (reviewed by Webster [61]) . The outgrowth of nerve fibers appears to be the initial event with naked nerve sprouts (lacking sheath cells) having been observed, for example, in the living tadpole tail fin (35,55,9) . Subsequently, Schwann cells of neural crest origin (35, 64) migrate along the nerve fibers and begin the process of ensheathment . At first, large groups of axons are surrounded by a few Schwann cells (45,42,28) . Subsequently, there is a burst of Schwann cell proliferation (57, 7) and invasion by the proliferating cells of the fascicles of naked axons (45, 21, among others) . Eventually, a sorting of axons occurs, with the largest axons (those destined to be myelinated) segregated into a 1 :1 relationship with the Schwann cells . Concomitant with the termination of Schwann cell proliferation, the myelin sheaths and connective tissue components of the peripheral nerve are formed.Cellular interactions between neurons and supporting cells are critical during development as well as subsequently (56,60) . To elucidate the nature of these interactions, a number of experi-J. CELL BIOLOGY

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Section: Proliferation Of Celts During Degenerationmentioning

confidence: 96%

Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury.

Salzer¹,

Bunge²

1980

The Journal of Cell Biology

393

213

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“…This denervation-very little TFR increa t in the proximal part of the nerve, coinciding induced cell proliferation and the preceding transferrin receptor expression could be mediated by myelin breakdown products (Salzer and Bunge, 1980a,b;Yoshino et al, 1984). Compared to the relatively myelin-rich sciatic nerve, there appears to be only little cell proliferation after injury of unmyelinated nerves (Joseph, 1947(Joseph, , 1950Abercrombie et a/., 1959;Romine et al, 1976). In vitro experiments have also indicated that myelin digested by macrophages presents a strong mitogenic stimulus for the cultured Schwann cells (Baichwal et al, 1988).…”

Section: Transferrin Receptor Expression On Sch Wann Cellsmentioning

confidence: 99%

Transferrin Receptor Expression and Iron Uptake in the Injured and Regenerating Rat Sciatic Nerve

Raivich

Graeber

Gehrmann

et al. 1991

Eur J of Neuroscience

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Iron-saturated transferrin is a ubiquitous growth factor that plays a critical role in cellular iron uptake, growth and proliferation. Here we have studied the expression and distribution of transferrin receptors and iron uptake following injury of the rat sciatic nerve. Axotomy led to a massive but transient increase (days 2 - 9, maximum day 4) in [125I]transferrin binding at the site of the injury and in the distal, denervated part of the crushed or resected sciatic nerve, shortly preceding the time course of cellular proliferation (Friede and Johnstone, Acta Neuropathol, 7, 218 - 231, 1967; Jurecka et al., Acta Neuropathol, 32, 299 - 312, 1975). An additional, transient increase in specific binding was observed during reinnervation after reconnection of the resected sciatic nerve. Immunocytochemistry using the Ox-26 monoclonal antibody revealed strong and simultaneous expression of the transferrin receptor protein on two different cell types: on a subpopulation of blood-borne macrophages invading the injured peripheral nerve and on Schwann cells reacting to denervation and reinnervation. In addition, studies using intravenously injected radioactive iron (59Fe3+) showed a massive increase in endoneural iron uptake confined to the lesion site and to the distal part of the axotomised sciatic nerve, parallel to the time course of reactive transferrin receptor expression. Since iron is an essential cofactor of a number of key enzymes needed in energy metabolism and DNA synthesis, these data suggest that the induction of transferrin receptor expression may play an important role in the regulation of cellular growth and proliferation during peripheral nerve regeneration.

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“…Using cultured neurites and Schwann cells from rat dorsal root ganglia, Salzer and Bunge (15) demonstrated that the developmental signal was provided by the growing axon. Morphological studies (1,12,21) suggested that the increase in the number of cells during Waller-Jan degeneration was proportional to the size of the myelinated fiber. Indirect evidence for myelin as a mitogenic signal was provided by Salzer and Bunge (15) who found that only Schwann cells that had produced myelin proliferated during Wallerian degeneration.…”

mentioning

confidence: 99%

Differential proliferative responses of cultured Schwann cells to axolemma- and myelin-enriched fractions. I. Biochemical studies.

Yoshino¹,

Dinneen²,

Lewis³

et al. 1984

The Journal of Cell Biology

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Cultured rat Schwann ceils were treated for 72 h with axolemma-and myelinenriched fractions prepared from rat brainstem.[3H]Thymidine was added to the cultures 48 h before the termination of the experiment. Although, both fractions produced a dosedependent uptake of label into Schwann cells, the shape of the dose response curves and rates at which [3H]thymidine was incorporated were different. The axolemma-enriched fraction produced a sigmoid dose response curve with a Hill coefficient of 2.05. The dose response curve for myelin rose sharply and saturated at a level that was ~50% of the maximal response observed with axolemma. Schwann cells that had been treated with axolemma exhibited little change in the rate of [3H]thymidine incorporation from 36-72 h after the addition of the membranes. In contrast, Schwann cells accumulated label three times faster during the 48-72-h period following the addition of myelin to the cultures when compared with the rate during the preceding 12-h interval. Furthermore, the mitogenic activity of the myelin-enriched fraction was decreased by the addition of ammonium chloride, a lysosomal inhibitor, whereas the activity of the axolemmal fraction was not impaired.

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Further Studies in Changes of Nuclear Population in Degnerating Non-Myelinated and Finely Myelinated Nerves

Cited by 28 publications

References 0 publications

Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury.

Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury.

Transferrin Receptor Expression and Iron Uptake in the Injured and Regenerating Rat Sciatic Nerve

Differential proliferative responses of cultured Schwann cells to axolemma- and myelin-enriched fractions. I. Biochemical studies.

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