Further studies and biological activities of macromolecular protein R-Phycoerythrin from Portieria hornemannii

Senthilkumar, N; Kurinjimalar, C.; Thangam, Ramar; Suresh, Veeraperumal; Kavitha, G.; Gunasekaran, Palani; Rengasamy, Ramasamy

doi:10.1016/j.ijbiomac.2013.08.004

Cited by 53 publications

(29 citation statements)

References 31 publications

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“…Functional food substances from marine sources are not only pharmacologically effective but also antagonize dysregulated targets in cellular signaling pathways to exert their antineoplastic activities (Tarapore, Siddiqui, & Mukhtar, ). Phycobiliproteins are an important group of water soluble natural pigment molecules extracted from the red and blue green algae and they are nontoxic proteins with potent anticancer, antioxidant, and anti‐inflammatory properties (Bei, Guang‐Ce, Chen‐Kui, & Zhen‐gang, ; Ravi, et al, ; Senthilkumar, Kurinjimalar, et al, ; Thangam, et al, ). Hence, an investigation was carried out to evaluate the anticancer potential of RPERPE obtained from P. hornemannii (Lyngbye) Silva against HCC using male Wistar albino rats as models.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we have demonstrated the anticancer effect of R‐Phycoerythrin (R‐PE) obtained from P. hornemannii against HepG2 cell line (Senthilkumar, Kurinjimalar, et al, ). In another study, we reported a nanotheranostic formulation using R‐PE as therapeutic and diagnostic molecule targeting triple negative breast cancer cells (Thangam, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Senthilkumar¹,

Thangam

Murugan

et al. 2018

J Food Biochem

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The present investigation evaluates the hepato‐protective potential of R‐phycoerythrin (R‐PE)‐rich protein extract obtained from Portieria hornemannii (Lyngbye) Silva against H2O2‐induced hepatotoxicity using HepG2 cells (in vitro) and male Wistar albino Hepatocellular carcinoma (HCC) rats (in vivo). H2O2 treatment led to dose‐dependent decreases in cell viability of HepG2, which was ameliorated by the treatment with R‐PE‐rich extract. In vivo studies showed that all the vital parameters including enzymatic and nonenzymatic antioxidants as well as liver marker enzymes were affected in the experimentally induced HCC male Wistar rats upon exposure to the N‐diethylnitrosamine (DEN), a carcinogen. However, there was moderate to better restoration of such parameters in the carcinoma rats treated with R‐PE‐rich protein extract. Practical applications Therapeutic alternatives originating from food or food supplements are gaining popularity as “nutritional therapy” and they are well studied for their chemo‐preventive effects. Investigations of several food‐derived bioactive compounds revealed their ability to antagonize dysregulated targets in cellular signaling pathways to exhibit antineoplastic activities. Isolation of bioactive molecules present in marine food products and determination of their broad range pharmaceutical activity by means of deducing specific molecular targets as well as establishing minimal toxicity to normal tissues could aid in treatment of cancer. Phycobiliproteins are an important group of pigment molecules extracted from the red and blue green algae. A study was envisaged to evaluate the anticancer potential of RPERPE (R‐PE‐rich protein extract) obtained from P. hornemannii (Lyngbye) Silva against HCC using male Wistar albino rats as models. This study offers the scope of using the R‐PE‐rich protein extract of P.hornemannii for developing therapeutic anticancer formulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Senthilkumar¹,

Thangam

Murugan

et al. 2018

J Food Biochem

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“…S3). These results are consistent with thermal stability of R‐PE from Portieria hornemannii (Senthilkumar et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Phycoerythrin can be divided into three types, depending on their absorption spectrum: R‐phycoerythrin (R‐PE, peaks at 499 and 565 nm with a 545 nm shoulder), B‐phycoerythrin (B‐PE, peaks at 545 and 565 nm with a 499 nm shoulder) and C‐phycoerythrin (C‐PE, peaks at 565 nm) (Galland‐Irmouli et al ., ). The antioxidant and in vitro anticancer properties of R‐PE from Portieria hornemannii have been characterised (Senthilkumar et al ., ), and it has been reported that C‐PE can protect against HgCl 2 ‐caused oxidative stress (Cano‐Europa et al ., ). Traditionally, R‐PE was purified using a combination of several techniques, including ammonium sulphate precipitation followed by chromatography on ion exchange, hydroxylapatite and gel filtration columns (Bermejo Román et al ., ; Rossano et al ., ; Fu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae Bangia fusco‐purpurea

Sun

et al. 2015

Int J of Food Sci Tech

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R-phycoerythrin (R-PE) was purified from the red algae Bangia fusco-purpurea after 35-50% ammonium sulphate fractionation followed by ion-exchange column chromatography on DEAE-Sepharose, resulting in a purity (A 565 /A 280 ) ratio of 5.1. The circular dichroism spectroscopy results suggested that the structure of R-PE is predominately helical. The antioxidant activity of R-PE was studied and revealed changes in conformation and antioxidant activity at different temperatures and pH values. After in vitro-simulated gastrointestinal (GI) digestion of R-PE, the scavenging activity of ABTS radical (EC 50 , 769.9 lg mL À1 ), DPPH radical (EC 50 , 421.9 lg mL À1 ), hydroxyl radical (EC 50 , 32.4 lg mL À1 ) and reducing power (A 700 = 0.5, 625.8 lg mL À1 ) were measured. Gel filtration chromatography analysis showed that the molecular weight distribution of the final GI digest that still contained high antioxidant activity was <3 kDa. Our present results indicate that digestion-resistant antioxidant peptides of R-PE may be obtained by in vitro GI proteinases degradation.

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“…However the quantity and purity of the pigments are likely to vary among these solvents. Since pigments are highly sensitive to oxygen, temperature, pH, and light, and as the stability of the pigments differs in various solvents, it is important to store the pigments using appropriate preservatives [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Methods of phycobiliprotein extraction from Gracilaria crassa and its applications in food colourants

et al. 2015

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Further studies and biological activities of macromolecular protein R-Phycoerythrin from Portieria hornemannii

Cited by 53 publications

References 31 publications

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae Bangia fusco‐purpurea

Methods of phycobiliprotein extraction from Gracilaria crassa and its applications in food colourants

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