Further Purification of Cholecystokinin and Pancreozymin.

Jorpes, Erik; Mutt, Viktor; Toczko, Kazimierz; Sparrow, D. R.

doi:10.3891/acta.chem.scand.18-2408

Cited by 86 publications

(13 citation statements)

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“…It is possible, therefore, that CCK was not alone responsible for the stimulation of motor activity, but the effect could be ascribed to other closely related peptides, such as motilin (5). However, in animal experiments, highly purified CCK (21) has been shown to cause a contraction of the small intestine in vitro and in vivo (9,18). This increase in motility was effected by the pure CCK and not by contaminating substances.…”

Section: Discussionmentioning

confidence: 99%

Effects of Secretin and Cholecystokinin on Motor Activity of Human Jejunum

Dollinger¹,

Berz²,

Raptis³

et al. 1975

Digestion

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The effects of intravenous administration of secretin and cholecystokinin (CCK) on motility of the human jejunum were investigated with pressure-sensitive radiotelemetering capsules. Secretin inhibited and CCK stimulated the spontaneous motor activity of this part of the small intestine. Raising the dosages of the two hormones caused increasing effects. It seems likely, therefore, that both hormones play a role in the regulation of intestinal motility in man.

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Section: Discussionmentioning

confidence: 99%

Effects of Secretin and Cholecystokinin on Motor Activity of Human Jejunum

Dollinger¹,

Berz²,

Raptis³

et al. 1975

Digestion

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“…the full molecule or small fragments or a combination of both. It is therefore more meaningful to express results in terms of biological activity (mIDU/ml), although for comparison the molar equivalent of the full molecule in pmol/ml has been placed in parentheses after quoted biological units using conversion factors based on 3000 IDU/mg (Jorpes, Mutt & Toczko, 1968) and considering the molecular weight of CCK to be 3940.…”

Section: Apparatusmentioning

confidence: 99%

An Improved Method for Estimating Cholecystokinin in Human Serum

Marshall¹,

Egberts²,

Johnson³

1978

Journal of Endocrinology

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Because of difficulties encountered in setting up radioimmunoassays for cholecystokinin (CCK) a sensitive and reliable biological method for estimating this hormone is still needed. The principles of such a biological technique and an improvement to it have already been described, but the serum levels of CCK reported were high and the technique required further refinement and validation. The strips of rabbit gall-bladder used to estimate the concentration of CCK increased in sensitivity to standard solutions of CCK over a 6--8 period before stabilizing, but a single sample of serum increased the sensitivity of the strips of gall-bladder to their maximum immediately. These two problems were eliminated by 'priming' the strips of gall-bladder by exposure to two serum samples before exposure to the standard solutions used for production of a dose--response curve. Thirdly, it was discovered that some non-peptide substances in serum possessed CCK-like activity; by extracting all the small peptides from serum with dextran-coated charcoal the residual activity could be measured and subtracted from the total CCK activity. Finally, the activity of CCK in the serum increased during processing before freezing. This increase was eliminated by taking the blood samples into aprotinin which has been shown to cause dramatic reduction in CCK activity in some experiments. When all these factors were taken into account and the technique suitably modified, the mean level of CCK in the serum of ten normal fasting subjects was found to be 28 milli Ivy Dog units/ml (2.4 pmol/ml), which is only one third of that reported previously.

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“…Independently of the question of pancreozymin, Ivy & Oldberg (1928) had earlier obtained evidence for an intestinal hormone, cholecystokinin, which regulated gall bladder contraction. In 1964, Jorpes & Mutt isolated from hog duodenum a peptide with the properties of both cholecystokinin and pancreozymin (Jorpes, Mutt & Toczko, 1964). Throughout the isolation of the peptide these two hormonal activities had moved in parallel, which indicated that they were separate properties of the same molecule (Jorpes & Mutt, 1966).…”

Section: Introductionmentioning

confidence: 95%