1979
DOI: 10.1002/jsfa.2740301109
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Further purification and characterisation of a new amylase found in barley

Abstract: An amylase, previously detected in barley and described as a new barley amylase, has been further purified by immuno-affinity and ion exchange chromatography on CMcellulose. Analysis by isoelectricfocusing and immunochemical techniques showed that the enzyme preparation did not contain the normal a-and P-amylases usually found in barley and malt. The enzyme had a very low isoelectric point (ca pH 3.0) and was identified as an a-amylase on the basis of its action pattern on amylose.

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