Further observations on the fine structure of orally exposed and carious human dental cementum

Furseth, Randi

doi:10.1016/0003-9969(71)90138-5

Cited by 49 publications

(20 citation statements)

References 22 publications

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“…A similar condition may exist in vivo. Electron microscopic studies on decalci fied specimens have shown denatured collagen fibrils and collagen loss, but also intact collagen fibrils in the superficial layer of both active and arrested denti nal lesions [Johansen and Parks, 1961;Frank et al, 1964;Sarnat and Massler, 1965;Furseth, 1971], As suming that the intact fibrils were still partly mineral ized, this might indicate that removal of the mineral fractions which are in close association with the fibrils is a prerequisite for denaturation and further degrad ation of collagen. The retention of these mineral frac tions might also be of relevance for lesion arrestment/ remineralization.…”

Section: Discussionmentioning

confidence: 99%

“…When the lesion progressed, the apparently firmly bound mineral disappeared more or less simultaneously with degradation of the col lagenous matrix by proteinases and/or by nonenzymatic processes [Johansen and Parks, 1961;Frank et al, 1964;Selvig, 1968]. In cementum caries, an almost synchronous breakdown of the inorganic and organic components has been observed [Furseth, 1971;Frank et al, 1989]. Young and Massler [1963] have shown that, during dentinal caries, a change in the chemical composition of the original organic matrix results in resistance against acid dissolution and proteolysis, presumably by interactions with components of bac terial or salivary origin.…”

Section: Introductionmentioning

confidence: 99%

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Susceptibility of the Collagenous Matrix from Bovine Incisor Roots to Proteolysis after in vitro Lesion Formation

Klont

Cate

1991

Caries Res

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The susceptibility of the organic matrix from permanent bovine incisor roots to proteolytic breakdown after in vitro lesion formation was investigated. Root surfaces were exposed to 0.1 M acetic acid, pH 4.0, to produce erosive lesions or to 0.1 M lactic acid, 0.2 mM methane hydroxy diphosphonate, pH 5.0, to produce subsurface lesions. After demineralization, the roots were treated with a bacterial collagenase. The quantitity of enzyme-degradable collagen in the root tissue was found to be proportional to the calcium released during demineralization, until a plateau value was reached at calcium concentrations in solution of 3.3 mM at pH 4.0 and 2.7 mM at pH 5.0. The degradability of collagen was found to be substantially less in subsurface lesions than in erosive lesions. The presence of cementum-free areas did not affect the results. These findings suggest that the mineral component of the roots is composed of several fractions which differ in their solubility properties in weak acids.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Susceptibility of the Collagenous Matrix from Bovine Incisor Roots to Proteolysis after in vitro Lesion Formation

Klont

Cate

1991

Caries Res

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“…In a more advanced stage of the caries process, the ap parently firmly bound mineral disappeared more or less simultaneously with destruction of the collage nous matrix [Johansen and Parks, 1961;Frank et al, 1964;Selvig, 1968]. In cementum caries, an almost synchronous breakdown has been demonstrated of the inorganic and organic components [Furseth, 1971;Frank et al, 1989].…”

mentioning

confidence: 97%

Remineralization of Bovine Incisor Root Lesions in vitro: The Role of the Collagenous Matrix

Klont

Cate²

1991

Caries Res

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This study deals with the role of collagen in the remineralization of bovine incisor root lesions. The degradability of the collagenous matrix after in vitro remineralization was also investigated. The root surfaces were exposed to 0.1 M acetic acid, pH 4.0, to produce erosive lesions or to 0.1 M lactic acid, 0.2 mM methane hydroxy diphosphonate, pH 5.0, to produce subsurface lesions. After demineralization, the roots were subjected to remineralization, with or without prior treatment with a bacterial collagenase to remove accessible collagen. Remineralization was carried out in solutions with a Ca/P_i molar ratio of 1.67, and [Ca]·[P_i] concentration products of 0.6, 1.35 or 2.4 mM², with all solutions having an ionic strength of 0.15 M, and a pH of 7.0. After remineralization, the roots that were not treated with collagenase were incubated with the enzyme. The results indicate that remineralization of erosive lesions is surface-controlled and that of subsurface lesions diffusion-controlled. For both lesion types it was shown that the removal of collagen before remineralization did not affect the rate of mineral deposition in the root lesions. Nevertheless, remineralization in those lesions where accessible collagen had not been removed resulted in a significant reduction in the amount of degradable collagen. The observations of this study suggest that remineralization did not occur by nucleation of mineral on the organic matrix but rather by growth of residual crystals in the partially demineralized root tissue.

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“…[2][3][4] Remineralization is the repair of established carious lesions. Structurally intact collagen may provide a good scaffold to promote remineralization.…”

Section: Introductionmentioning

confidence: 99%

Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

Benjamin

Sharma²,

Thomas³

et al. 2012

The Journal of Contemporary Dental Practice

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Objective: Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation. Materials and methods:Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05).Results: GSE revealed less demineralization and more remineralization compared with other groups. Conclusion: GSE promotes remineralization of artificial root caries lesions.Clinical significance: The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium.

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Further observations on the fine structure of orally exposed and carious human dental cementum

Cited by 49 publications

References 22 publications

Susceptibility of the Collagenous Matrix from Bovine Incisor Roots to Proteolysis after in vitro Lesion Formation

Susceptibility of the Collagenous Matrix from Bovine Incisor Roots to Proteolysis after in vitro Lesion Formation

Remineralization of Bovine Incisor Root Lesions in vitro: The Role of the Collagenous Matrix

Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

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