Further Observations Concerning the Periodic Acid Oxidation of Hydroxylamine Derivatives<sup>1</sup>

Emery, Thomas; Neilands, J. B.

doi:10.1021/jo01050a531

Cited by 46 publications

(32 citation statements)

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“…Periodate oxidation of 0.1 mg of the desferrihydroxamate by the method of Emery & Neilands (1960& Neilands ( , 1962) gave a product with an absorption peak at 267nm. The intact desferrisiderophore had no absorption peak near this wavelength at the same concentration.…”

Section: Isolation and Properties Of The Siderophore From Agrn Quadrmentioning

confidence: 99%

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Armstrong¹,

Baalen²

1979

Journal of General Microbiology

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Section: Isolation and Properties Of The Siderophore From Agrn Quadrmentioning

confidence: 99%

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Armstrong¹,

Baalen²

1979

Journal of General Microbiology

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“…After 72 h of incubation, quantification of hydroxamate-type siderophore was performed by the method of Emery and Neilands (7,8). The readings of absorbance at 264 nm were converted to hydroxamic acid concentrations based on a standard curve (data not shown).…”

mentioning

confidence: 99%

Quantification of Siderophore and Hemolysin from Stachybotrys chartarum Strains, Including a Strain Isolated from the Lung of a Child with Pulmonary Hemorrhage and Hemosiderosis

Vesper

Dearborn

Elidemir

et al. 2000

Appl Environ Microbiol

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A strain of Stachybotrys chartarum was recently isolated from the lung of a pulmonary hemorrhage and hemosiderosis (PH) patient in Texas (designated the Houston strain). This is the first time that S. chartarum has been isolated from the lung of a PH patient. In this study, the Houston strain and 10 strains of S. chartarum isolated from case (n ‫؍‬ 5) or control (n ‫؍‬ 5) homes in Cleveland were analyzed for hemolytic activity, siderophore production, and relatedness as measured by random amplified polymorphic DNA analysis.The fungus Stachybotrys chartarum (Ehrenb. ex Link) Hughes(ϭ S. atra Corda) has been associated with a number of human health problems, including potentially fatal pulmonary hemorrhage and hemosiderosis (PH) in infants (3,4,5,9,10,16). Recently, Elidemir et al. (6) isolated a strain of S. chartarum (designated here as the Houston strain) from the lungs of a child with progressive respiratory symptoms and PH. S. chartarum was also found in the child's water-damaged home. The child recovered after removal from the house and subsequent remediation of the home.In this study, hydroxamate-type siderophore production and hemolytic activity by case and control strains from Cleveland were quantified and compared to those of the Houston strain. Random amplified polymorphic DNA (RAPD) analysis of the Houston strain was also compared with those of case and control strains from Cleveland (22).Quantification of siderophore production and hemolytic activity by S. chartarum when incubated with human blood. Five strains of S. chartarum isolated from PH control houses in and five from case houses (51-08, 51-11, 58-02, 58-06, and 63-07) (15) and the Houston strain were used. The strains of S. chartarum were grown on wet wallboard pieces as previously described (22). Human blood used in this study was taken from one of the authors by his physician using 7-ml vacuum tubes containing sodium heparin (Becton Dickinson, Franklin Lakes, N.J.), and the tubes were then refrigerated overnight. The next day the plasma was separated from the packed red blood cells (RBC) and the buffy coat containing the white blood cells was removed and discarded. To 250 ml of Trypticase soy broth (Becton Dickinson, Sparks, Md.) was added 1.5 ml of the packed RBC and 2 ml of plasma, and then the sample was mixed continuously while 10-ml aliquots were dispensed into 50-ml polypropylene tubes (Corning Inc., Corning, N.Y.). Approximately 4 ϫ 10 4 conidia of each strain were added to each of three replicate tubes. The tubes were placed on an incubatorshaker

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“…Almost all naturally occurring hydroxamic acids are secondary, RCON(0H)R'. The acyl group of these substance is also quantitatively split with periodic acid to yield the free acid (50,52). Using this reaction, the acyl groups of ferrichrome and ferrichrome A were identified as acetic and trans-,f?-methylglutaconic acids, respectively.…”

Section: B Periodate Oxidationmentioning

confidence: 99%

“…The appearance of an absorption peak during periodic acid reaction is unique for secondary hydroxamic acids and N-alkylhydroxylamines. This absorption peak has found use as a sensitive method to detect the secondary hydroxamic acid grouping in biological compounds (52). Chelated iron protects the hydroxamate group from reaction with periodic acid.…”

Section: B Periodate Oxidationmentioning

confidence: 99%

Hydroxamic Acids of Natural Origin

Emery

1971

Advances in Enzymology - And Related Areas of Molecular Biology

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Further Observations Concerning the Periodic Acid Oxidation of Hydroxylamine Derivatives¹

Cited by 46 publications

References 0 publications

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Quantification of Siderophore and Hemolysin from Stachybotrys chartarum Strains, Including a Strain Isolated from the Lung of a Child with Pulmonary Hemorrhage and Hemosiderosis

Hydroxamic Acids of Natural Origin

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Further Observations Concerning the Periodic Acid Oxidation of Hydroxylamine Derivatives1

Cited by 46 publications

References 0 publications

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Iron Transport in Microalgae: the Isolation and Biological Activity of a Hydroxamate Siderophore from the Blue-Green Alga Agmenellum quadruplicatum

Quantification of Siderophore and Hemolysin from Stachybotrys chartarum Strains, Including a Strain Isolated from the Lung of a Child with Pulmonary Hemorrhage and Hemosiderosis

Hydroxamic Acids of Natural Origin

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Further Observations Concerning the Periodic Acid Oxidation of Hydroxylamine Derivatives¹