Further investigations on populations of the deep-water blue and red shrimp Aristeus antennatus (Risso, 1816) (Decapoda, Dendrobranchiata), as inferred from Amplified Fragment Length Polymorphism (AFLP) and mtDNA analyses

Abstract

The aim of this study was to integrate existing mitochondrial DNA data relating to the deepsea blue and red shrimp Aristeus antennatus (Risso, 1816) with data obtained by Amplified Fragment Length Polymorphism (AFLP). A total of 145 AFLP polymorphic loci were scored in 236 specimens collected from one Atlantic and seven Mediterranean sample sites. AMOVA results revealed that the overall genetic variation among-populations was lower (11.81%) than withinpopulations (88.19%). The genetic variation between the Atlantic and Mediterranean samples was found to be not significant ( CT = −0.007; N.S.), indicating that the transition area between the Atlantic Ocean and the Mediterranean Sea does not act as a barrier to gene flow. Bayesian analysis also demonstrated the absence of genetic differentiation between the Atlantic and Mediterranean populations and within the Mediterranean basin. The results are in agreement with those previously published using mitochondrial markers. Some considerations on the life history traits of the species are discussed. Key words. -AFLP, mtDNA, Aristeus antennatus, Atlanto-Mediterranean region RIASSUNTO Lo scopo del lavoro è stato quello di integrare dati precedenti ottenuti dall'analisi del DNA mitocondriale nel gambero viola Aristeus antennatus (Risso, 1816) con dati ottenuti dall'analisi di un marcatore nucleare (Amplified Fragment Length Polymorphism, AFLP). In totale sono stati identificati 145 loci polimorfici AFLP in 236 esemplari raccolti da sette località del Mediterraneo e da un sito dell'Oceano Atlantico. I risultati dell'analisi AMOVA hanno rivelato che la variazione genetica tra le popolazioni è inferiore (11,81%) alla variazione intra-popolazionale (88,19%). Il 3 )

Genetic structure and population connectivity of the blue and red shrimp Aristeus antennatus

“…These results differ from the conclusions of Lo Brutto et al . 32 using mtDNA and amplified fragment length polymorphisms (AFLPs) in A. antennatus , as they reported no significant genetic variation between the Atlantic and the Mediterranean populations. Considering that the depth of maximum abundance of A. antennatus adults (600–800 m) 3 falls below the maximum sill depth in the Strait of Gibraltar (284 m at a width of ~30 km) 21 , the dispersal of adults is restricted, although possible planktonic larval drift by surface currents would not 33–36 .…”

Isolation By Distance (IBD) signals in the deep-water rose shrimp Parapenaeus longirostris (Lucas, 1846) (Decapoda, Panaeidae) in the Mediterranean Sea

“…Allelic frequencies at AFLP loci were calculated from the observed frequencies of the fragments using the Bayesian approach, as proposed by Zhivotovsky (1999) for diploid species. Statistics relating to gene diversity were computed, using AFLP-SURV 1.0 software, strictly following the treatment proposed by Lynch and Milligan (1994); total gene diversity H T (Nei, 1987) was calculated by pooling all individuals into a single metapopulation. H E , within-population Nei's gene diversity (Nei, 1987), was also computed.…”

“…The aim of this Paper is to assess the genetic population structure of P. longirostris, employing nuclear (Amplified Fragment Length Polimorphism, AFLP) and mitochondrial (Control Region sequences) markers to assess whether distinct stocks occur in the Mediterranean Sea. The comparison of these two markers enable us to make a more comprehensive investigation into genetic diversity; specifically, highly resolving DNA fingerprinting techniques (such as AFLP markers and mtDNA control regions) have proven to be valuable in estimating genetic diversity in natural populations (Lo Brutto et al, 2012;García-Merchán et al, 2012).…”

Modelling giant red shrimp larval dispersal in the Sardinian seas: density and connectivity scenarios

“…() reported a substantial relationship between the life history of A. antennatus and local oceanographic processes, in that the surface and mid‐water currents could play an important part in the horizontal displacement of the larvae. Several genetic studies conducted using molecular techniques revealed no distinct sub‐populations and low genetic heterogeneity, probably because of the dispersal capabilities of red shrimps during both adult and larval stages (Sardà et al ., , ; Maggio et al ., ; Roldán et al ., ; Marcias et al ., ; Cannas et al ., ; Fernández et al ., , ; Lo Brutto et al ., ). Recently, Marra et al .…”