The effect of the application of CO2 to extend the cold
storage of raw and pasteurized milk on the
content of fat-soluble vitamins of milk was investigated.
CO2-treated milk (pH 6.2) was compared
with a control (unacidified) milk. CO2-treated and
control raw milk samples were stored at 4 °C
for 4 days. CO2-treated milk was then vacuum
degasified, and both control and treated samples
were pasteurized and stored at 4 °C for 7 days. CO2
addition inhibited the growth of microorganisms
in raw milk without affecting the stability of vitamin A (retinol and
β-carotene) and vitamin E
(α-tocopherol). Acidity and pH data indicated that subsequent
vacuum degasification and
pasteurization on a pilot scale partially removed CO2,
making milk acceptable for liquid consumption.
However, the residual CO2 present extended the
cold-storage period of pasteurized milk by inhibiting
bacterial survivors without detrimental effects on retinol,
β-carotene, and α-tocopherol. Slightly
higher (not statistically significant, p > 0.05)
concentrations of retinol, β-carotene, and
α-tocopherol
were detected during cold storage in raw and pasteurized
CO2-treated milk with respect to the
control milk, which could be related to a certain protective effect of
the CO2.
Keywords: Milk; carbon dioxide; psychrotrophs; cold storage; fat-soluble
vitamin