Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells

Raabe, Oksana; Shell, K.; Würtz, Antonia; Reich, Christine Maria; Wenisch, Sabine; Arnhold, Stefan

doi:10.1007/s11259-011-9480-z

Cited by 29 publications

(18 citation statements)

References 35 publications

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“…The expression of specific markers characteristic of embryonic stem cells have been described before for equine BM-MSCs [47] and AT-MSCs [40]. However, to our knowledge, this is the first work that compares the gene expression of the pluripotency markers OCT4 , NANOG and SOX2 in equine AT-MSCs and BM-MSCs that were exposed to different oxygen concentrations.…”

Section: Discussionmentioning

confidence: 90%

Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

et al. 2012

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BackgroundMesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are being applied to equine cell therapy. The physiological environment in which MSCs reside is hypoxic and does not resemble the oxygen level typically used in in vitro culture (20% O2). This work compares the growth kinetics, viability, cell cycle, phenotype and expression of pluripotency markers in both equine BM-MSCs and AT-MSCs at 5% and 20% O2.ResultsAt the conclusion of culture, fewer BM-MSCs were obtained in hypoxia than in normoxia as a result of significantly reduced cell division. Hypoxic AT-MSCs proliferated less than normoxic AT-MSCs because of a significantly higher presence of non-viable cells during culture. Flow cytometry analysis revealed that the immunophenotype of both MSCs was maintained in both oxygen conditions. Gene expression analysis using RT-qPCR showed that statistically significant differences were only found for CD49d in BM-MSCs and CD44 in AT-MSCs. Similar gene expression patterns were observed at both 5% and 20% O2 for the remaining surface markers. Equine MSCs expressed the embryonic markers NANOG, OCT4 and SOX2 in both oxygen conditions. Additionally, hypoxic cells tended to display higher expression, which might indicate that hypoxia retains equine MSCs in an undifferentiated state.ConclusionsHypoxia attenuates the proliferative capacity of equine MSCs, but does not affect the phenotype and seems to keep them more undifferentiated than normoxic MSCs.

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Section: Discussionmentioning

confidence: 90%

Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

et al. 2012

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“…Currently, the most widely used tissue sources for isolation of MSCs in equine medicine are bone marrow (BM) and subcutaneous adipose tissue (AT) [6,9,10]. …”

Section: Introductionmentioning

confidence: 99%

Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties

et al. 2013

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BackgroundThe treatment of tendon lesions with multipotent mesenchymal stromal cells (MSCs) is widely used in equine medicine. Cell sources of MSCs include bone marrow, as well as solid tissues such as adipose tissue. MSCs can be isolated from these solid tissues either by enzymatic digestion or by explant technique. However, the different preparation techniques may potentially influence the properties of the isolated MSCs. Therefore, the aim of this study was to investigate and compare the effects of these two different methods used to isolate MSCs from solid tissues.Equine adipose tissue, tendon and umbilical cord matrix served as solid tissue sources of MSCs with different stiffness and density. Subsequent to tissue harvest, MSCs were isolated either by enzymatic digestion with collagenase or by explant technique. Cell yield, growth, differentiation potential and tendon marker expression were analysed.ResultsAt first passage, the MSC yield was significantly higher in enzymatically digested tissue samples than in explanted tissue samples, despite a shorter period of time in primary culture. Further analysis of cell proliferation, migration and differentiation revealed no significant differences between MSCs isolated by enzymatic digestion and MSCs isolated by explant technique. Interestingly, analysis of gene expression of tendon markers revealed a significantly higher expression level of scleraxis in MSCs isolated by enzymatic digestion.ConclusionsBoth isolation techniques are feasible methods for successful isolation of MSCs from solid tissues, with no major effects on cellular proliferation, migration or differentiation characteristics. However, higher MSC yields were achieved in a shorter period of time by collagenase digestion, which is advantageous for the therapeutic use of MSCs. Moreover, based on the higher level of expression of scleraxis in MSCs isolated by enzymatic digestion, these cells might be a better choice when attempting tendon regeneration.

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“…Many studies have described the isolation of MSCs from a range of canine tissues, including adipose tissue (Neupane et al, 2008;Vieira et al, 2010;Kisiel et al, 2012;Reich et al, 2012;Takemitsu et al, 2012), bone marrow (Kisiel et al, 2012;Reich et al, 2012;Takemitsu et al, 2012), muscle (Kisiel et al, 2012), periosteum (Kisiel et al, 2012) and synovium (Zhang et al, 2013). Similarly diverse sources have been identified for equine MSCs (Hegewald et al, 2004;Koch et al, 2007;Crovace et al, 2010;Raabe et al, 2011;Ranera et al, 2012;Burk et al, 2013;Barberini et al, 2014;De Schauwer et al, 2014), as well as caprine (Murphy et al, 2003), ovine (Jager et al, 2006;Mrozik et al, 2010) and porcine (Wang et al, 2008;Cao et al, 2011;Miernik and Karasinski, 2012).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem cell therapies for treating osteoarthritis: Prescient or premature?

Whitworth

Banks

2014

The Veterinary Journal

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Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells

Cited by 29 publications

References 35 publications

Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties

Stem cell therapies for treating osteoarthritis: Prescient or premature?

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