Further evidence that <scp>E</scp>‐cadherin is not a tumour suppressor gene in invasive ductal carcinoma of the breast: an immunohistochemical study

Rakha, Emad A.; Teoh, Tsu Kuan; Lee, Andrew H.S.; Nolan, Chris; Ellis, Ian O.; Green, Andrew

doi:10.1111/his.12066

Cited by 30 publications

(35 citation statements)

References 53 publications

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“…Although it has been reported that ductal carcinomas may show an aberrant loss of expression of E-cadherin and β-catenin, this is typically focal and observed in high-grade tumours [13]. The similarity of the cytonuclear morphology and immunoprofile between PC-like areas and classic ILC areas present in the surrounding breast tissue provided further evidence to support the lobular nature of the lesions.…”

Section: Discussionmentioning

confidence: 89%

Invasive Lobular Carcinoma Mimicking Papillary Carcinoma: A Report of Three Cases

Rakha

Abbas²,

Sheeran³

2016

Pathobiology

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Encapsulated and solid papillary carcinomas (EPCs and SPCs) are considered historically as a special form of ductal carcinoma in situ. Invasive lobular carcinoma (ILC) is characterised by a discohesive growth pattern. There are several variants of ILC, but, as yet, no papillary subtype has been identified. Here we report 3 cases of ILC presenting as papillary carcinoma (PC) with a typical solid papillary growth pattern. One case was reported on core biopsy as EPC (B5a). The 3 ensuing resection specimens showed features typical of SPC with a circumscribed malignant epithelial proliferation containing fibrovascular cores and surrounded, at least focally, by a thick fibrous capsule. The lobular nature of these tumours was confirmed on the resection specimens by the absence of E-cadherin and β-catenin membrane expression. The invasive nature was confirmed by the presence of entrapped fat cells, the absence of myoepithelial cells and focal merging of the solid papillary areas with classic ILC at the periphery. Of note, 1 case was a recurrent carcinoma without an in situ component. Conclusion: This study provides further evidence that EPC and SPC represent a unique growth pattern of breast carcinomas rather than reflecting the in situ or invasive nature of the tumour, and that ILC can acquire a papillary growth pattern.

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Section: Discussionmentioning

confidence: 89%

Invasive Lobular Carcinoma Mimicking Papillary Carcinoma: A Report of Three Cases

Rakha

Abbas²,

Sheeran³

2016

Pathobiology

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“…Further, the TMA technique carries some drawbacks, such as sampling errors and loss of information due to missing tissue cores. Notably, false negative results have been reported for biomarkers studied on TMA sections [53], but it is not known whether this is applicable to Ki67. Regarding ER and PR expression, we used a threshold of 10% for molecular sub-classification according to national guidelines at the time, as compared to the 1% threshold recommended by the present St Gallen guidelines.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Ki67 Expression across Distinct Categories of Breast Cancer Specimens: A Population-Based Study of Matched Surgical Specimens, Core Needle Biopsies and Tissue Microarrays

et al. 2014

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IntroductionTumor cell proliferation in breast cancer is strongly prognostic and may also predict response to chemotherapy. However, there is no consensus on counting areas or cut-off values for patient stratification. Our aim was to assess the matched level of proliferation by Ki67 when using different tissue categories (whole sections, WS; core needle biopsies, CNB; tissue microarrays, TMA), and the corresponding prognostic value.MethodsWe examined a retrospective, population-based series of breast cancer (n = 534) from the Norwegian Breast Cancer Screening Program. The percentage of Ki67 positive nuclei was evaluated by visual counting on WS (n = 534), CNB (n = 154) and TMA (n = 459).ResultsThe median percentage of Ki67 expression was 18% on WS (hot-spot areas), 13% on CNB, and 7% on TMA, and this difference was statistically significant in paired cases. Increased Ki67 expression by all evaluation methods was associated with aggressive tumor features (large tumor diameter, high histologic grade, ER negativity) and reduced patient survival.ConclusionThere is a significant difference in tumor cell proliferation by Ki67 across different sample categories. Ki67 is prognostic over a wide range of cut-off points and for different sample types, although Ki67 results derived from TMA sections are lower compared with those obtained using specimens from a clinical setting. Our findings indicate that specimen specific cut-off values should be applied for practical use.

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“…The primary antibodies, manufacturers, clones, dilutions, antigen retrieval, and scoring system [13][14][15][16][17][18][19][20] details are outlined in Table 2. Immunohistochemistry (IHC) assays were performed using the ultraView Universal DAB Detection Kit and on the BenchMarck XT and BenchMarck ULTRA (Roche/Ventana, Oro Valley, AZ) instruments.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Immunohistochemical and molecular profiling of histologically defined apocrine carcinomas of the breast

et al. 2015

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Further evidence that E‐cadherin is not a tumour suppressor gene in invasive ductal carcinoma of the breast: an immunohistochemical study

Abstract: These results demonstrate that E-cadherin-negative ductal carcinoma is rare, and in these cases p120 and β-catenin maintained their membranous localization, suggesting a functional E-cadherin-membrane complex.

Cited by 30 publications

References 53 publications

Invasive Lobular Carcinoma Mimicking Papillary Carcinoma: A Report of Three Cases

Invasive Lobular Carcinoma Mimicking Papillary Carcinoma: A Report of Three Cases

Evaluation of Ki67 Expression across Distinct Categories of Breast Cancer Specimens: A Population-Based Study of Matched Surgical Specimens, Core Needle Biopsies and Tissue Microarrays

Immunohistochemical and molecular profiling of histologically defined apocrine carcinomas of the breast

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