Further evidence of direct uptake of sucrose by Saccharomyces cerevisiae in batch culture.

Barford, J. P.; Mwesigye, Patrick K.; Phillips, Peter J.; Jayasuriya, Dharini; Blom, Ingela

doi:10.2323/jgam.39.389

Cited by 10 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could mean that there is a sucrose transport system that is induced when the cells are adapted on sucrose for a long time (up to 14 days). This is in agreement with our earlier reports on sucrose metabolism in S. cerevisiae (3,14,17). It has been reported that under glucose repression, the secretion of internal invertase increases while the external invertase remains low and practically constant (12).…”

Section: And Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Saccharomyces cerevisiae was grown on different concentrations of sucrose and glucose mixtures after adapting on sucrose. The yeast cells were found to have two different mechanisms by which sucrose was utilised: hydrolysis outside the cell membrane and direct transport into the cells. The mechanism by which sucrose was utilised depended on the initial concentration of glucose in the mixture and the adaptation state of the cells. In both cases, glucose was utilised first and invertase secretion was repressed when the glucose concentration was higher than 2 g l-'. The major important finding in our results is that, for fully sucrose-adapted cells, even in the presence of a repressive glucose concentration, the yeast cells were able to utilise sucrose, and most importantly, without first being hydrolysed to its constituent monosaccharides. Previously, it has been thought that direct transport of sucrose contributes only a small part of the process of utilisation of sucrose, but as our results indicate it can be the major mechanism of sucrose utilisation in S. cerevisiae if the cells are adapted on sucrose for long periods, glucose concentrations in the medium are high and/or invertase activities are limiting.The initial steps in the utilisation of sugars by yeast is either the passage of intact sugar molecules across the cell membrane or the hydrolysis outside the cell followed by entry of all or some of the hydrolytic products into the cell. Sugar transport into microbial cells across the plasmalemma can take place by active transport, facilitated diffusion and group translocation (19).Sucrose utilisation involves its hydrolysis outside the yeast cell by extracellular enzyme invertase ($-D-fructosidase) into glucose and fructose which are then transported into the cells and metabolised. Most Saccharomyces cerevisiae strains have two forms of invertase: an external large invertase, which is a glycoprotein, and an internal small invertase (9,15). Some strains of S. cerevisiae have been reported to transport sucrose directly into the cell (14,17,20).

show abstract

Section: And Discussionsupporting

confidence: 83%

“…The strain of baker's yeast used in this work was Saccharomyces cerevisiae 248 UNSW 703100 (3,14,17). This yeast was maintained on malt agar slopes and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The results indicated that GmSUS had the capability to increase UDP-glucose, which was a sugar donor essential for the enzymatic glycosylation reaction. This also reconfirmed the idea that sucrose could be ingested directly by S. cerevisiae [42][43][44][45][46], allowing in situ recycling of UDP-glucose to be achieved within the cell. Hence, it was crucial for the microbial synthesis of glycosides to optimize the production of UDP-sugars in the host organisms through a range of regulations and enhancements.…”

Section: Efficient Synthesis Of Salidroside By In-situ Udp-glucose Re...supporting

confidence: 57%

Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae

Zhou,

Zhang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Salidroside, a valuable phenylethanoid glycoside, is obtained from plants belonging to the Rhodiola genus, known for its diverse biological properties. At present, salidroside is still far from large-scale industrial production due to the lower titer and higher process cost. In this study, we constructed a biosynthetic pathway from L-tyrosine to salidroside in Saccharomyces cerevisiae. To increase sali-droside production, we for the first time constructed an in vivo UDP-glucose regeneration system that sucrose synthase GmSUS worked in conjunction with UDP-glucose transferase RrUGT33 to improve the UDP-glucose availability with inexpensive sucrose as the consumed substrate. Finally, the molar conversion rate of L-tyrosine could reach 95%. To further reduce the cost, co-culture with E. coli that synthesized L-tyrosine de novo resulted in an 81% reduction in the final substrate cost per 1 kg of salidroside compared to the initial cost. The final titer of salidroside obtained 3.8 g/L and was the highest titer achieved at the Shake-Flask-Level in the existing reports. And this marked the first successful synthesis of salidroside in an in situ enhanced UDP-glucose system using sucrose. This accomplishment laid a robust groundwork for further investigations into the synthesis of other notable glycosides and natural compounds.

show abstract

“…Furthermore, since the metabolism of sucrose in the yeast S. cerevisiae differs from that of its monomers (or a mixture of them) only in the step of disaccharide breakdown, when compared to growth on glucose and/or fructose, it can be speculated that active transport of sucrose accelerates the sugar uptake rate, and, consequently, the higher growth rate observed on the disaccharide. In fact, Barford and co-workers (Orlowski and Barford 1991; Barford et al 1993) demonstrated that the superior growth of S. cerevisiae 248 UNSW 703100 — fully adapted to the culture medium for 20 - 250 generations — on sucrose, in comparison to a mixture of its monomers, was due to the direct uptake of sucrose molecules by actively growing yeast cells, which is faster than the passive transport of hexoses.…”

Section: Resultsmentioning

confidence: 99%

Aerobic growth physiology ofSaccharomyces cerevisiaeon sucrose is strain-dependent

Rodrigues

Wahl

Gombert

2021

Preprint

View full text Add to dashboard Cite

Present knowledge on the quantitative aerobic physiology of the yeast Saccharomyces cerevisiae during growth on sucrose as sole carbon and energy source is limited to either adapted cells or to the model laboratory strain CEN.PK113-7D. To broaden our understanding of this matter and open novel opportunities for sucrose-based biotechnological processes, we characterized three strains, with distinct backgrounds, during aerobic batch bioreactor cultivations. Our results reveal that sucrose metabolism in S. cerevisiae is a strain-specific trait. Each strain displayed a distinct extracellular hexose concentration and invertase activity profiles. Especially, the inferior maximum specific growth rate (0.21 h-1) of the CEN.PK113-7D strain, with respect to that of strains UFMG-CM-Y259 (0.37 h-1) and JP1 (0.32 h-1), could be associated to its low invertase activity (0.04 to 0.09 U mgDM-1). Moreover, comparative experiments with glucose or fructose alone, or in combination, suggest mixed mechanisms of sucrose utilization by the industrial strain JP1, and points out the remarkable ability of the wild isolate UFMG-CM-259 to grow faster on sucrose than on glucose in a well-controlled cultivation system. This work hints to a series of metabolic traits that can be exploited to increase sucrose catabolic rates and bioprocess efficiency.

show abstract

Further evidence of direct uptake of sucrose by Saccharomyces cerevisiae in batch culture.

Cited by 10 publications

References 14 publications

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae

Aerobic growth physiology ofSaccharomyces cerevisiaeon sucrose is strain-dependent

Contact Info

Product

Resources

About