Further evidence for paternal DNA transmission in gynogenetic grass carp

Deciphering male influence in gynogenetic Pengze crucian carp (Carassius auratus var. pengsenensis): insights from Nanopore sequencing of structural variations

“…Specifically, we identified MSSVs in the exonic regions of five genes (polyunsaturated fatty acid 5-lipoxygenase, chondroadherin-like a, transmembrane protease serine 13-like, UNC93-like protein MFSD11, uncharacterized LOC127957002) exclusively in homologous offspring, but not in heterologous offspring. This is in contrast to other studies that have reported the presence of male inheritance in heterologous offspring ( Mao et al, 2020 ). The reasons for this discrepancy may include a limited sample size, stringent filtering criteria, or variability in sequencing data quality, making it challenging to identify in heterologous offspring in the study.…”

“…The observed variation indicates the emergence of novel SVs, which may result from a variety of mechanisms. These include sporadic homologous recombination and high-frequency gene conversion during the maturation of oocytes ( Wang et al, 2022 ), the suppression of DNA double-strand break formation and homologous recombination in oocytes ( Lu et al, 2022 ), or the incorporation of male genetic fragments during the gynogenetic process ( Zhang et al, 1999 ; Zhao et al, 2004a ; Mao et al, 2020 ; Ding et al, 2021 ; Zhao et al, 2021 ). The process of recombination, depending on its accuracy, plays a dual role: inaccurate recombination can lead to the introduction of new genetic variations, whereas precise recombination has the potential to correct existing genetic errors ( Burssed et al, 2022 ).…”

“…However, the presence of MSSVs is contingent upon whether the male contribution is homologous or heterologous, with homologous contributions exerting a greater genetic influence. Recent research, such as gynogenetic reproduction experiments in grass carp ( Ctenopharyngodon idellus ) using inactivated koi carp ( Cyprinus carpio haematopterus ) sperm, has unveiled recombined genes, like HoxC6b , resulting from the inheritance of male DNA ( Mao et al, 2020 ) . Similarly, in natural gynogenetic silver carp, it was discovered that homologous male microchromosomes could be inherited by offspring, playing a pivotal role in enhancing offspring genetic diversity ( Ding et al, 2021 ; Zhao et al, 2021 ).…”

“…Notably, gynogenetic offspring are not exact clones of the mother, as evidenced by diverse offspring traits and the presence of male offspring in various sperm-stimulated combinations (Shu et al, 2001;Zhao and Chen, 2003;Zhou et al, 2020;Ding et al, 2021;Shi et al, 2022). Studies have confirmed the inheritance of male genetic material in offspring (Zhang et al, 1999;Zhao et al, 2004a;Mao et al, 2020;Ding et al, 2021;Zhao et al, 2021), challenging previous assumptions about genetic uniformity. Although homologous and heterologous male pronuclei do not fuse with the female pronucleus, the interaction between them may facilitate male genetic material transfer (Yang, 2004;Zhao et al, 2021).…”

Molecular Characterization and Nutrition Regulation of the Neutral Amino Acid Transporter ASCT2 in Triploid Crucian Carp

“…Phylogenetic analysis was conducted using the sequences of SLC1A5 homologs from triploid crucian carp and other vertebrates according to the methods of Mao et al [22]. The nucleotide sequences of these species were derived from the National Center for Biotechnology Information database.…”