Further evidence for an ATP-driven sodium pump in the marine alga Tetraselmis (Platymonas) viridis

Balnokin, Yu. V.; Попова, Л. Г.; Gimmler, H.

doi:10.1016/s0176-1617(97)80118-6

Cited by 27 publications

(35 citation statements)

References 29 publications

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“…Several studies on salinity tolerance mechanism of marine algae have been conducted, and one of the major findings is that Na + -ATPase is the most important factor for salinity resistance among algae [33,34]. Short-term highor low-salinity shock had significant effect on PN and RR of S. thunbergii seedlings, specifically at 10 and 50 psu.…”

Section: Discussionmentioning

confidence: 99%

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)

Liang¹,

Sun²,

Wang³

et al. 2013

AJPS

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Sargassum thunbergii is of great economic and ecological value to sea cucumber cultures and seaweed beds. However, studies on photosynthesis and respiration of S. thunbergii are limited. In this study, a liquid-phase oxygen electrode system and a chlorophyll fluorescence spectrometer were used to determine the photosynthetic characteristics of S. thunbergii seedlings at various light intensities, temperatures, and salinities. The light-saturated net photosynthetic rates, light saturation points, and respiratory rates of germlings were investigated. Results showed that the increase in rate of light saturation point was slow in the first 4 d, rapidly increased from 5 d to 9 d, and then gently increased at the final few days of the 15 d indoor culture period. The photosynthetic rate or respiratory rate of the seedlings rapidly and significantly decreased when the temperature was <10˚C or >28˚C. Short-term high-or low-salinity shock had significant effect on the photosynthetic and respiratory rates of the seedlings, specifically at 10 and 50 psu. However, the photosynthetic and respiratory rates recovered to the normal levels after 24 h of recovery period, which demonstrated a powerful ion-transport system of the seedlings. These results provided reference for the artificial breeding of S. thunbergii.

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Section: Discussionmentioning

confidence: 99%

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)

Liang¹,

Sun²,

Wang³

et al. 2013

AJPS

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“…In T. viridis, in contrast, K + ions were neither required for ATP-dependent Na + translocation across the PM (Balnokin et al 1997) nor did they affect dephosphorylation of the Na + -ATPase pre-phosphorylated with c-[ 32 P]ATP in the presence of Na + .…”

Section: Introductionmentioning

confidence: 90%

“…Our previous studies of Na + transport in PM vesicles isolated from the marine alga T. viridis (Chlorophyta) have shown that the Na + -ATPase of this organism is related to the family of P-type cationtranslocating ATPases, but its transport characteristics are different from homologous enzymes found in animals and the marine alga H. akashiwo (Cryptophyta; Balnokin et al 1997). Exchange of Na + for K + is a feature of the Na + ,K + -ATPase from mammalian cells (for review, see Stein 1986) and, likely, of the Na + -transporting ATPase from H. akashiwo (Wada et al 1989).…”

Section: Introductionmentioning

confidence: 92%

“…During the last decade, Na + -ATPases were found to operate in the plasma membrane (PM) of two marine algal species, Tetraselmis (Platymonas) viridis Rouch. (Balnokin and Popova 1994;Balnokin et al 1997) and Heterosigma akashiwo (Wada et al 1989;Shono et al 1996). Na + -ATPases are of particular interest as the Na + -transporters fulfilling primary active Na + translocation across the PM.…”

Section: Introductionmentioning

confidence: 99%

“…Na + -ATPases are of particular interest as the Na + -transporters fulfilling primary active Na + translocation across the PM. This feature ensures that Na + -ATPases can be considered as an effective mechanism for Na + export from the cells in a broad pH range of the ambient solution, including the alkaline region when Na + extrusion out of the cells by the Na + /H + antiporter appears unfeasible because of thermodynamic limitations (Balnokin et al 1997; for review, see Gimmler 2000). Thus, Na + -ATPases contribute vitally to salt tolerance of algal cells.…”

Section: Introductionmentioning

confidence: 99%

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The Na + -translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na + /H + exchange

Balnokin

Попова

Pagis

et al. 2004

Planta

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Our previous investigations have established that Na+ translocation across the Tetraselmis viridis plasma membrane (PM) mediated by the primary ATP-driven Na+-pump, Na+-ATPase, is accompanied by H+ counter-transport [Y.V. Balnokin et al. (1999) FEBS Lett 462:402-406]. The hypothesis that the Na+-ATPase of T. viridis operates as an Na+/H+ exchanger is tested in the present work. The study of Na+ and H+ transport in PM vesicles isolated from T. viridis demonstrated that the membrane-permeant anion NO3- caused (i) an increase in ATP-driven Na+ uptake by the vesicles, (ii) an increase in (Na(+)+ATP)-dependent vesicle lumen alkalization resulting from H+ efflux out of the vesicles and (iii) dissipation of electrical potential, deltapsi, generated across the vesicle membrane by the Na+-ATPase. The (Na(+)+ATP)-dependent lumen alkalization was not significantly affected by valinomycin, addition of which in the presence of K+ abolished deltapsi at the vesicle membrane. The fact that the Na+-ATPase-mediated alkalization of the vesicle lumen is sustained in the absence of the transmembrane deltapsi is consistent with a primary role of the Na+-ATPase in driving H+ outside the vesicles. The findings allowed us to conclude that the Na+-ATPase of T. viridis directly performs an exchange of Na+ for H+. Since the Na+-ATPase generates electric potential across the vesicle membrane, the transport stoichiometry is mNa+/nH+, where m> n.

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Alkaliphilic and Alkali-Tolerant Algae

Gimmler¹,

Degenhard

2001

Algal Adaptation to Environmental Stresses

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Further evidence for an ATP-driven sodium pump in the marine alga Tetraselmis (Platymonas) viridis

Cited by 27 publications

References 29 publications

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)

The Na + -translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na + /H + exchange

Alkaliphilic and Alkali-Tolerant Algae

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Further evidence for an ATP-driven sodium pump in the marine alga Tetraselmis (Platymonas) viridis

Cited by 27 publications

References 29 publications

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of &lt;i&gt;Sargassum thunbergii&lt;/i&gt; (Sargassaceae, Phaeophyta)

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of &lt;i&gt;Sargassum thunbergii&lt;/i&gt; (Sargassaceae, Phaeophyta)

The Na + -translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na + /H + exchange

Alkaliphilic and Alkali-Tolerant Algae

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Product

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Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)

Impact of Environmental Factors on the Photosynthesis and Respiration of Young Seedlings of <i>Sargassum thunbergii</i> (Sargassaceae, Phaeophyta)