“…A halogen lamp (Sylvania, 100 W, FCR) was used in substage illumination for specimen location and focusing. The excitation sources used in the present study were a UV bulb (Osram G.M.B.H., Berlin, Germany; HBO 100) in incident illumination (2,8) and a pulsed dye laser (Synergetics Research, Princeton, N.J.; Chromabeam model 1070) in transmitted substage illumination. A helium-neon CW laser (Metrologic Instruments, Inc., Belmawr, N.J.; model 360) with low output was used for optical alignment of the pulsed dye laser.…”