Further development of a transcervical technique for artificial insemination in sheep using previously frozen semen

Buckrell, B.C.; Buschbeck, C.; Gartley, Cathy; Kroetsch, Tom; McCutcheon, W.; Martin, Joseph E.; Penner, W.; Walton, J.S.

doi:10.1016/0093-691x(94)90377-u

Cited by 40 publications

(20 citation statements)

References 5 publications

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“…The lambing rates resulting from the Guelph system ranged from 60% to 80% using fresh semen and from 40% to 70% of successfully penetrated ewes inseminated with frozen-thawed semen. The lambing rates were significantly higher for ewes bred during the traditional fall breeding seasons than during other times of the year [27]. Evidently, the Guelph system shows promise as a non-surgical alternative to laparoscopic intrauterine deposition of semen in ewes.…”

Section: The Guelph System For Tcaimentioning

confidence: 88%

“…It was also suggested that the diameter of the insemination instrument should not exceed the diameter of the narrowest section of the cervix [18,26]. This prompted Buckrell et al, [27] to develop a bent-tipped AI catheter using an 18-gauge stainless steel spinal needle with a Luer-Lok screw top hub, which was modified to attach to a screw on the anterior end of a bovine AI gun barrel. The apparatus is designed for 0.5-cc semen straws and shortened to a length of 28 cm.…”

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

“…The pointed end of the needle is soldered until blunt to reduce cervical trauma and bent at a 45° angle at 9.8 mm from the tip, a measure representing the average distance of greatest offset of the successive cervical rings [18]. The bent end was also drilled to create an aperture for fluid to pass through [27]. Other catheters that have been developed have a winding, helicoidal end.…”

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

“…[18]). (F)-a bent-tipped AI catheter developed by Buckrell et al [27], and using an 18-gauge stainless steel spinal needle with a blunt tip attached to a bovine AI gun barrel shortened to 28 cm. (G)-a tip of the Milovanov catheter, with characteristic helicoidal shape, attached to a 25-cm plastic tube [13].…”

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

“…A specific technique for ovine TCAI using the catheter described by Buckrell et al, [27] was developed at the University of Guelph and denoted the "Standardized Guelph system for TCAI". This technique involves positioning the ewe in dorsal recumbency using a hoof trimming cradle, a device colloquially known as a "sheep flipper" (Fig.…”

Section: The Guelph System For Tcaimentioning

confidence: 99%

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A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Candappa¹,

Bartlewski²

2011

TORSJ

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Artificial insemination (AI) and embryo transfer (ET) play an important role in the genetic improvement of sheep herds. This review primarily describes the methods that have recently been researched and/or adapted to circumvent the problematic anatomy of the ovine cervix, which prevents the widespread application of transcervical AI and ET in this species. A mechanism of cervical ripening at parturition and the endocrine influences on cervical tone are described as a backdrop to understanding the action of various pharmacological agents used to induce cervical dilation. Although several catheters have been designed to penetrate the ovine cervix, and a specific protocol has been developed for TCAI in sheep (Guelph Method), penetration rates and resulting pregnancy/lambing rates are still inconsistent and unpredictable. Hormonal dilation of the cervix may significantly improve the ease with which the ovine cervix can be traversed. Treatment with Cervidil ® , a dinoprostone-containing vaginal insert with a slow release mechanism, currently used to induce labor in women, could significantly improve cervical penetration in ewes without adverse effects on uterine contractility. This mode of drug delivery holds promise to result in the development of a safe method to induce cervical dilation for a broad range of transcervical reproductive manipulations in mammalian species including, but not limited to, AI, ET, embryo and oocyte recovery, treatment of ringwomb and transcervical surgical procedures. However, more studies are needed to evaluate the effects of hormonal methods on cervical morphology/pathomorphology, sperm/embryo transport and viability, and fertility in both cyclic and seasonally anovular ewes.

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Section: The Guelph System For Tcaimentioning

confidence: 88%

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

Section: Customization Of Catheters For Tcai/et In Sheepmentioning

confidence: 99%

Section: The Guelph System For Tcaimentioning

confidence: 99%

See 3 more Smart Citations

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Candappa¹,

Bartlewski²

2011

TORSJ

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Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

SÁNCHEZ‐PARTIDA,

WINDSOR,

EPPLESTON

et al. 1999

Journal of Andrology

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The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen frozen in diluents reported to improve postthaw motility. The percentages of motile and progressive spermatozoa were better when frozen in proline—or glycine betaine‐containing or HEPES‐based, rather than Trisbased, diluents (P < 0.01). The fertility of spermatozoa frozen in diluents containing proline or glycine betaine was slightly reduced, whereas when both compatible solutes were present, the reduction was more pronounced, in comparison with semen frozen in Tris‐ or HEPES‐based diluents (9.5 versus 71.1 and 66.6%; P < 0.01). Fertility of frozen—thawed spermatozoa was higher after laparoscopic insemination than after cervical or transcervical insemination (P < 0.01). Similarly, higher fertility was obtained after cervical insemination with fresh than with frozen‐thawed semen (32.4 versus 11.3%; P < 0.01). Furthermore, loss of embryos was lower after laparoscopic insemination of ewes with semen frozen in a Tris diluent than with semen frozen in proline diluents, in glycine betaine diluents, or in proline‐plus‐glycine betaine diluents (0.0 versus 26.0, 38.5, and 60.0%; P < 0.001). A wide variation in the postthaw percentage of motile (31.6–59.7%) and progressive (22.6–43.1%) spermatozoa and in the fertility of spermatozoa from individual rams was also observed after laparoscopic (29.2–59.7%) or cervical insemination (8.7–30.5%). Postthaw motility results from immediately after thawing and fertility results from experiments where intrauterine insemination was performed with semen frozen in proline‐ or glycine betaine—containing or HEPES‐ or Tris‐based diluents were pooled and subjected to a pairwise correlation procedure. The correlation analysis showed relationships between some of the motility characteristics (P < 0.01), but there were no relationships between the motility characteristics and fertility.

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Current and Future Assisted Reproductive Technologies for Mammalian Farm Animals

Hansen

2013

Advances in Experimental Medicine and Biology

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Reproduction in domestic animals is under control by man and the technologies developed to facilitate that control have a major impact on the efficiency of food production. Reproduction is an energy-intensive process. In beef cattle, for example, over 50 % of the total feed consumption required to produce a unit of meat protein is consumed by the dam of the meat animal (Anim Prod 27:367-379, 1978). Sows are responsible for about 20 % of the total feed needed to produce animals for slaughter (Adv Pork Prod 19:223-237, 2008). Accordingly, energy input to produce food from animal sources is reduced by increasing number of offspring per unit time a breeding female is in the herd. Using beef cattle as an example again, life-cycle efficiency for production of weaned calves is positively related to early age at puberty and short calving intervals (J Anim Sci 57:852-866, 1983). Reproductive technologies also dictate the strategies that can be used to select animals genetically for traits that improve production. Of critical importance has been artificial insemination (AI) (Anim Reprod Sci 62:143-172, 2000; Stud Hist Philos Biol Biomed Sci 38:411-441, 2007; Reprod Domest Anim 43:379-385, 2008; J Dairy Sci 92:5814-5833, 2009) and, as will be outlined in this chapter, emerging technologies offer additional opportunities for improvements in genetic selection. Given the central role of reproduction as a determinant of production efficiency and in genetic selection, improvements in reproductive technologies will be crucial to meeting the challenges created by the anticipated increases in world population (from seven billion people in 2011 to an anticipated nine billion by 2050; World population prospects: the 2010 revision, highlights and advance tables. Working Paper No. ESA/P/WP.220, New York) and by difficulties in livestock production wrought by climate change (SAT eJournal 4:1-23, 2007).The purpose of this chapter will be to highlight current and emerging reproductive technologies that have the potential to improve efficiency of livestock production. The focus will be on technologies that manipulate male and female gametes as well as the stem cells from which they are derived and the preimplantation embryo. While technology is crucial to other interventions in the reproductive process like control of seasonal breeding, hormonal regulation of ovulation, estrous cyclicity and pregnancy establishment, feeding to optimize reproduction, minimizing environmental stress, and selection of genes controlling reproduction, these will not be considered here. Rather the reader is directed to other chapters in this volume as well as some reviews on other aspects of artificial manipulation of reproduction (Reprod Fertil Dev 24:258-266, 2011; Reprod Domest Anim 43:40-47, 2008; Reprod Domest Anim 43:122-128, 2008; Soc Reprod Fertil Suppl 66:87-102, 2009; Comprehensive biotechnology, Amsterdam, pp 477-485; Dairy production medicine, Chichester, pp 153-163; Theriogenology 76:1619-1631, 2011; Theriogenology 76:1568-1582, 2011; Theriogenology...

show abstract

Further development of a transcervical technique for artificial insemination in sheep using previously frozen semen

Cited by 40 publications

References 5 publications

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

Current and Future Assisted Reproductive Technologies for Mammalian Farm Animals

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