Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts

Lin, Willy

doi:10.1104/pp.74.2.219

Cited by 83 publications

(45 citation statements)

References 21 publications

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“…The decline in endogenous NADPH levels when bean roots or corn roots are exposed to Fe(CN)63-suggests that NADPH is the physiological reductant (16,22). The physiological oxidant may be ferric salt (21,22) or 02 (7,14). Oxidoreductase activity has been detected 'Supported by a grant from the Natural Sciences and Engineering in plasma membrane enriched fractions from root cells (9,19) and hypocotyl segments (8), and evidence for a flavoprotein Cyt complex in the plasma membrane has been obtained (13,25).…”

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confidence: 99%

“…However, a proton electrochemical gradient across biological membranes may also be established by redox reactions involving the sequential operation of hydrogen and electron carriers (7,14 (12,21). A third model has recently been proposed in which plasma membrane redox activity transports only electrons to an exogenous acceptor.…”

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confidence: 99%

“…However, a proton electrochemical gradient across biological membranes may also be established by redox reactions involving the sequential operation of hydrogen and electron carriers (7,14). Electrogenic redox driven proton movements across chloroplast and mitochondrial membranes have been well characterized and do not require ATP.…”

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A Plasmamembrane Redox System and Proton Transport in Isolated Mesophyll Cells

Neufeld¹,

Bown²

1987

Plant Physiol.

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Substantial evidence indicates that a plasma membrane located, ATP requiring outwardly directed proton pump establishes a proton electrochemical gradient that is used to drive the transport of solutes into and out of plant cells ( 17,23,24). However, a proton electrochemical gradient across biological membranes may also be established by redox reactions involving the sequential operation of hydrogen and electron carriers (7,14 (12,21). A third model has recently been proposed in which plasma membrane redox activity transports only electrons to an exogenous acceptor. The resulting depolarization then activates the proton ATPase and accounts for the increase in H4 efflux observed on addition of Fe(CN)631 (18).Very little is known concerning the existence of plasma membrane redox activities in photosynthetic cells. The reduction of exogenous Fe(CN)63-by Elodea leaf cells and an associated decline in membrane potentials has been reported (1 1). Active ATP driven proton efflux and related transport processes are currently being investigated using isolated Asparagus mesophyll cells (3,5,20

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A Plasmamembrane Redox System and Proton Transport in Isolated Mesophyll Cells

Neufeld¹,

Bown²

1987

Plant Physiol.

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“…This is consistent with the distribution of redox and chelate-splitting activity investigated by Rensch and Bottger (1988) by means of staining techniques. NAD(P)H is discussed as a cytoplasmic electron donor for the plasma membrane redox system (Lin, 1984;Qiu et al, 1985;Kriiger and Bottger, 1988). It is an interesting coincidente that the cytoplasmic NAD(P)H level is considerably higher in rhizodermal cells than in other cells (Rensch and Bottger, 1988).…”

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Penetration by Artificial Electron Acceptors of the Plasma Membrane-Bound Redox System into Intact Zea mays L. Roots Investigated by Proton-Induced X-Ray Emission

et al. 1993

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Proton-induced x-ray emission was used to investigate the penetration of compounds of the membrane-impermeant electron acceptors hexabromoiridate IV, hexachloroiridate IV, and hexacyanoferrate 111 into corn (Zea mays 1.) roots. Maps of the heavy element distribution in cross-sections of fixed, epoxy-embedded roots showed for hexabromoiridate IV small amounts of Br in samples treated for 24 h with concentrations normally used in physiological experiments (0.02 mM). After treatment with high concentrations (0.8 mM) of these complexes, Fe and Ir as well as Br were found in root cross-sections. In samples taken at a distance of 5 mm behind the root tip, we found an even distribution of Fe, Ir, and Br over the whole cross-section. In samples taken 15 mm behind the root tip, about 99% of both Br and Ir was confined to the rhizodermal cell layer. The distribution did not change with the complex used. These data are consistent with the view that apoplastic diffusion of the electron acceptors was blocked by the hypodermal Casparian band.

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“…Ofparticular interest woud be a redox chain able to function as an adjunct or alternative to ATPase in energization of the plasmalemma for nutrient transport. Evidence for such a redox system is gaining wider credence (5).…”

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Evidence for a Plasmalemma Redox System in Sugarcane

Thom¹,

Maretzki²

1985

Plant Physiol.

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A plasmalemma-bound NADH-dependent redox system has been identified in protoplasts isolated from cell suspensions of sugacane. This system oxidized NADH as well as NADPH, increased 02 consumpti 3-fold, and increased the pH of the external medium while the cytoplasmkc pH was decreased. In the presence of NADH, ferricyanide was rapidly reduced and the external medium was acidified. The uptake rates of K+, 3-Omethylglucose, leucine, and arginine were all decreased in the presence of NADH.A redox system localized within the plasmalemma of higher plants is an appealing concept for which there is at present still little direct evidence. Nevertheless, convincing data have been obtained from corn root (4) and oat root preparations (8).Additional indirect evidence supporting the functioning of a redox chain in the plasmalemma of plants has been reported (1, 2). The possible significance of oxidation-reduction in the plasmalemma was considered in a review by L6w and Crane (6) who suggested that redox reactions may control glycolytic functions, peroxidation of unsaturated lipids, and other cell functions via sulfhydryl sites. A trans-membrane electron transfer has also been proposed for iron reduction in bean roots (12). Ofparticular interest woud be a redox chain able to function as an adjunct or alternative to ATPase in energization of the plasmalemma for nutrient transport. Evidence for such a redox system is gaining wider credence (5).The addition of NADH to intact corn roots or protoplasts greatly increases 02 consumption (4) and NADH oxidation can be coupled to the reduction of added ferricyanide (2). Moreover, Lin (5) has demonstrated that K+ influx, particularly at low concentrations, is stimulated by the addition of NADH to corn root protoplasts, is accompanied by proton efflux, and causes an increase in the membrane potential.Our interest in a possible relationship between a redox function and sugar transport across the plasmalemma originated with an observation that membrane preparations from sugarcane cells showed NADH bound to the plasmalemma and a promotion of its oxidation to NAD in the presence ofglucose (9). In the present investigation we wanted to determine whether the observations made by Lin (4, 5) in corn root protoplasts could be extended to another species, such as sugarcane. Our results revealed some similarities in the behavior of these two species, but also some important differences that cast doubt on the significance of an NADH-linked redox chain to nutrient transport across the plasmalemma in sugarcane.

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Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts

Cited by 83 publications

References 21 publications

A Plasmamembrane Redox System and Proton Transport in Isolated Mesophyll Cells

A Plasmamembrane Redox System and Proton Transport in Isolated Mesophyll Cells

Penetration by Artificial Electron Acceptors of the Plasma Membrane-Bound Redox System into Intact Zea mays L. Roots Investigated by Proton-Induced X-Ray Emission

Evidence for a Plasmalemma Redox System in Sugarcane

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