Further characterization of marek's disease virus‐infected lymphocytes. I. <i>In vivo</i> infection

Calnek, B. W.; Schat, K. A.; Ross, L. J. N.; Shek, William R.; Chen, C-L. H.

doi:10.1002/ijc.2910330318

Cited by 106 publications

(70 citation statements)

References 27 publications

(22 reference statements)

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“…Cloned MDV isolates CU-2 (low oncogenicity) and JM-16 (moderate oncogenicity) have been described (Smith & Calnek, 1973;Calnek et al, 1984). All inocula for these experiments were from stocks of infected chicken kidney cell cultures stored at -196 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Spleens were collected aseptically and processed as reported by Calnek et al (1984). Briefly, spleen cells were gently forced through an autoclavable 60 ~tm mesh (Tetco, Elmsford, N.Y., U.S.A.), washed once in phosphate-buffered saline (PBS) pH 7.3 and centrifuged over Ficoll-Paque (Pharmacia) at 400 g for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…Lymphocytes were suspended in LM-Hahn medium at a concentration of 5 x 106/ml and incubated in a humidified, 5 % CO 2 atmosphere at 41 °C for 24 or 48 h as described previously (Calnek et aL, 1982(Calnek et aL, , 1984.…”

Section: Methodsmentioning

confidence: 99%

“…), an early cytolytic infection of the primary lymphoid organs (bursa of Fabricius, spleen and thymus) occurs; the predominant targets are B cells (Shek et al, 1983). This is followed by a persistent latent infection of mostly Ia-bearing T cells (Calnek et al, 1984). Lymphomas may develop in some~hickens several weeks or months later depending on factors such as genetic constitution, age at infection of the host and the strain of MDV.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Immunocompetence on the Establishment and Maintenance of Latency with Marek's Disease Herpesvirus

Buscaglia

Calnek

Schat

1988

Journal of General Virology

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SUMMARYMarek's disease virus (MDV) infections normally have an early cytolytic phase in lymphoid organs at 3 to 6 days post-infection followed by a period of latent infection. Little is known about the mechanisms that govern latency with herpesvirus infections, including Marek's disease (MD). To investigate the importance of immunocompetence for either the establishment or the maintenance of latency in MD, immunosuppressive treatments were applied prior to infection with MDV or after latency was established. These included cyclosporin (Cs) or betamethasone (BM) treatments, neonatal thymectomy plus cyclophosphamide treatment (Tx/Cy), and infection at a young age before full competence. The effect of all the treatments was determined by examining tissues and spleen cells for evidence of virus replication before and after cultivation in vitro. Induced (Cs or Tx/Cy treatments) or natural (infection at a young age) incompetence resulted in prolonged and more widespread early cytolytic infection. Immunosuppression by Cs after latency had developed resulted in a reappearance of cytolytic infection in the spleen and it enhanced the cytolytic infection in the thymus and the bursa of Fabricius. After immunosuppression with Cs, cytolytic infection was found mostly in T cells, although many of the virus-positive cells did not have markers for either T cells or B cells. Immunosuppression by BM after latency had developed also resulted in the reappearance of cytolytic infection in the spleen but only at a very low level. These results suggest that immunocompetence is required for the establishment and maintenance of latency.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Immunocompetence on the Establishment and Maintenance of Latency with Marek's Disease Herpesvirus

Buscaglia

Calnek

Schat

1988

Journal of General Virology

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“…By comparison, resistance in line 6 1 (B2 MHC haplotype) is strongly associated with the MDV-1 locus (see earlier). It has been postulated that resistance resides at the level of target cell numbers and their susceptibility to MDV infection and is evident during the lytic phase of infection (16,32).…”

mentioning

confidence: 99%

Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Kaiser

Underwood

Davison

2003

J Virol

187

114

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The production of cytokine mRNAs, in addition to viral DNA, was quantified by real-time quantitative reverse transcription-PCR (RT-PCR) (cytokines) or PCR (virus) in splenocytes during the course of Marek's disease virus (MDV) infection in four inbred chicken lines: two resistant (lines 6 1 and N) and two susceptible (lines 7 2 and P). Virus loads were only different after 10 days postinfection (dpi), increasing in susceptible lines and decreasing in resistant lines. Gamma interferon (IFN-␥) mRNA was expressed by splenocytes from all infected birds between 3 and 10 dpi, associated with increasing MDV loads. For other cytokines, differences between lines were only seen for interleukin-6 (IL-6) and IL-18, with splenocytes from susceptible birds expressing high levels of both transcripts during the cytolytic phase of infection, whereas splenocytes from resistant birds expressed neither transcript. These results indicate that these two cytokines could play a crucial role in driving immune responses, which in resistant lines maintain MDV latency but in susceptible lines result in lymphomas.

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Neoplastic Diseases

Nair¹,

Gimeno²,

Dunn³

et al. 2019

Diseases of Poultry

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Further characterization of marek's disease virus‐infected lymphocytes. I. In vivo infection

Cited by 106 publications

References 27 publications

Effect of Immunocompetence on the Establishment and Maintenance of Latency with Marek's Disease Herpesvirus

Effect of Immunocompetence on the Establishment and Maintenance of Latency with Marek's Disease Herpesvirus

Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Neoplastic Diseases

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