Further characterization of degenerated human cartilage with differential scanning calorimetry

Tóth, Kálmán; Sohár, G.; Pallagi, Edina; Szabó‐Révész, Piroska

doi:10.1016/j.tca.2007.07.013

Cited by 19 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes correlated with the water content of the samples. Due to the increased number of samples acquired for our studies, the results were much better reproducible than results in the literature, and the difference between the normal and diseased samples was significant Tóth et al, 2007]. The newly established thermogravimetric protocol that we used was sufficient for compositional thermoanalytical study of normal and degenerative human hyaline cartilage.…”

Section: Resultsmentioning

confidence: 76%

See 1 more Smart Citation

Characterization of Live and Experimentally Degenerated Hyaline Cartilage with Thermal Analysis

Sohár¹,

Szabó‐Révész²,

Tóth³

et al. 2012

Osteoarthritis - Diagnosis, Treatment and Surgery

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 76%

“…Previous thermoanalytical studies used cadaver samples for the investigation as normal human hyaline cartilage. All samples that were extracted for our studies were obtained during live surgeries and were macroscopically intact Tóth et al, 2007]. There is no previous report in the literature of examining normal cartilage from live surgery.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Live and Experimentally Degenerated Hyaline Cartilage with Thermal Analysis

Sohár¹,

Szabó‐Révész²,

Tóth³

et al. 2012

Osteoarthritis - Diagnosis, Treatment and Surgery

View full text Add to dashboard Cite

“…In the first case, a series of secondary frequency-independent peaks in the range of 70-100 • C (Figure 9c) was revealed due to melting of crystalline regions of collagen. The motions of the main and side chains of collagen molecules become significant in this region when a tensile force is applied, causing an irreversible spatial reorganization within the cartilage matrix [38,58]. This behaviour is in agreement with the different structural organization that collagen has been reported to be composed of: (i) an unstable crystalline region with little orientation that melts at low temperatures (80-180 • C), (ii) a natural amorphous region associated to second-order phase transitions that melts at around 120 • C, and (iii) a stable crystalline region with a melting point at 200 • C [59].…”

Section: Multi-frequency Tensile and Compressive Loading Of Nsmentioning

confidence: 99%

Thermo-Mechanical Behaviour of Human Nasal Cartilage

et al. 2020

View full text Add to dashboard Cite

The aim of this study was to undergo a comprehensive analysis of the thermo-mechanical properties of nasal cartilages for the future design of a composite polymeric material to be used in human nose reconstruction surgery. A thermal and dynamic mechanical analysis (DMA) in tension and compression modes within the ranges 1 to 20 Hz and 30 °C to 250 °C was performed on human nasal cartilage. Differential scanning calorimetry (DSC), as well as characterization of the nasal septum (NS), upper lateral cartilages (ULC), and lower lateral cartilages (LLC) reveals the different nature of the binding water inside the studied specimens. Three peaks at 60–80 °C, 100–130 °C, and 200 °C were attributed to melting of the crystalline region of collagen matrix, water evaporation, and the strongly bound non-interstitial water in the cartilage and composite specimens, respectively. Thermogravimetric analysis (TGA) showed that the degradation of cartilage, composite, and subcutaneous tissue of the NS, ULC, and LLC take place in three thermal events (~37 °C, ~189 °C, and ~290 °C) showing that cartilage releases more water and more rapidly than the subcutaneous tissue. The water content of nasal cartilage was estimated to be 42 wt %. The results of the DMA analyses demonstrated that tensile mode is ruled by flow-independent behaviour produced by the time-dependent deformability of the solid cartilage matrix that is strongly frequency-dependent, showing an unstable crystalline region between 80–180 °C, an amorphous region at around 120 °C, and a clear glass transition point at 200 °C (780 kJ/mol). Instead, the unconfined compressive mode is clearly ruled by a flow-dependent process caused by the frictional force of the interstitial fluid that flows within the cartilage matrix resulting in higher stiffness (from 12 MPa at 1 Hz to 16 MPa at 20 Hz in storage modulus). The outcomes of this study will support the development of an artificial material to mimic the thermo-mechanical behaviour of the natural cartilage of the human nose.

show abstract

“…This was suggested to be due to increased amounts of collagen and increased secondary bindings between collagen fibres. DSC has also been used to investigate other orthopaedic tissues such as cartilage17 and intervertebral discs 18…”

mentioning

confidence: 99%

Torn human rotator cuff tendons have reduced collagen thermal properties on differential scanning calorimetry

Chaudhury

Holland

Porter

et al. 2011

Journal Orthopaedic Research

View full text Add to dashboard Cite

The cause of the high failure rates often observed following rotator cuff tendon repairs, particularly massive tears, is not fully understood. Collagen structural changes have been shown to alter tendon thermal and mechanical properties. This study aimed to form a quantitative rather than qualitative assessment, of whether differences in collagen structure and integrity existed between small biopsies of normal, small, and massive rotator cuff tears using differential scanning calorimetry. Thermal properties were measured for 28 human biopsies taken intra-operatively from normal, small, and massive rotator cuff tendon tears in this powered study. Denaturation temperatures are represented by T onset (8C) and T peak (8C). The T onset is proposed to represent water-amide hydrogen bond breakage and resulting protein backbone mobility. T peak reportedly corresponds to the temperature at which the majority of proteins fall out of solution. Denaturation enthalpy (DH) should correlate with the amount of triple helical structure that is denatured. Fluorescence and confocal microscopy allowed quantitative validation. Small and massive rotator cuff tears had significantly higher T onset , T peak , and DH compared to controls. Polarized light microscopy of torn tendons confirmed greater collagen structural disruption compared to controls. These novel findings suggest greater quantifiable collagen structural disruption in rotator cuff tears, compared to controls. This study offers insight into possible mechanisms for the reduced strength of torn tendons and may explain why repaired tendons fail to heal. ß 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:1938Res 29: -1943Res 29: , 2011.Keywords: shoulder; rotator cuff; tendon; collagen denaturation; imaging American census data estimates that 17 million people potentially face the risk of disability from rotator cuff failure. 1 As rotator cuff tears become more prevalent with age, 2 an increasingly aging population means that the healthcare costs are likely to become greater for this disorder.Repairs of massive rotator cuff tendon tears have higher failure rates than small tears. 3 A multistage model of rotator cuff tears has been suggested, wherein tear size has been shown to affect both the cellular and extracellular matrix composition of the torn tendon edge. 4 Further understanding of changes in the extracellular matrix composition of rotator cuff tendon tears may offer insight into differences in healing and failure rates, and help to guide research into treatment strategies. Characterizing human rotator cuff tears can enhance our understanding of the complex degeneration occurring in chronic tears, which animal models cannot fully replicate.Differential scanning calorimetry (DSC) is a useful technique to characterize structural properties of very small (0.5 mg, approximately 2 Â 2 Â 2 mm) human tendon biopsies that are sampled intraoperatively as thermal properties are inherent to a material and hence much less affected by ...

show abstract

Further characterization of degenerated human cartilage with differential scanning calorimetry

Cited by 19 publications

References 18 publications

Characterization of Live and Experimentally Degenerated Hyaline Cartilage with Thermal Analysis

Characterization of Live and Experimentally Degenerated Hyaline Cartilage with Thermal Analysis

Thermo-Mechanical Behaviour of Human Nasal Cartilage

Torn human rotator cuff tendons have reduced collagen thermal properties on differential scanning calorimetry

Contact Info

Product

Resources

About