Further characterization of C6-aldehyde-forming system in alfalfa cells cultured in vitro.

Abstract

VolatileC6-aldehydes such as hexanal, m-3-hexenal and Jrarcs-2-hexenal, along with the corresponding alcohols, characterize flavors of plant tissues and are found in a variety of plants.1} Lipoxygenase and hydroperoxide lyase (HPO lyase) are responsible for the enzymatic formation of these C6-aldehydes from linoleic and linolenic acids originating from lipids.2) In a previous paper we reported that cultured green cells of alfalfa had the ability to produce the C6-aldehydes from linoleic and linolenic acids.3) The present paper describes further characterization of the C6-aldehyde-forming enzyme system of the cultured alfalfa cells.

C6-aldehyde formation from linolenic acid in fruit cells cultured in vitro

“…A direct correlation of C6-aldehyde forming activities and chlorophyll content was demonstrated by the same authors [5,14]. These observations may be generalized with respect to results with white and green calli of apple cv.…”

Photosynthetic Photon Flux, Photoperiod, and Temperature Effects on Emissions of (Z)-3-hexenal, (Z)-3-hexenol, and (Z)-3-hexenyl Acetate from Lettuce

“…Sekiya et al (1983a) reported a positive correlation between HL activity and chlorophyll content in the green leaves of various plants, but LOX activity seemed to affect the production of C 6 aldehydes. LOX activity of cultured green alfalfa cells is lightdependent, but HL activity is only slightly light-dependent (Sekiya et al, 1983b). Sekiya et al (1984) demonstrated that LOX activity is higher at higher temperatures in tea leaves, and ADH activity was low at high temperature.…”