Further characterization of a morphogenetic mutant of the foot-and-mouth disease virus

Giachetti, Cristina; Carrillo, Elisa; Campos, Rodolfo Héctor

doi:10.1016/0168-1702(86)90052-3

Cited by 3 publications

(3 citation statements)

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“…APS were raised in guinea pigs against FMDV 01 Caseros original strain (OpO) as previously described (14).…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of viral proteins. Labeled virus-specific proteins were immunoprecipitated from the CE with APS (PRN70: 3.7) as previously described (14). The immunoprecipitated proteins were analyzed in 10% polyacrylamide-sodium dodecyl sulfate (SDS) gels by using a discontinuous Trisglycine buffer system (19).…”

Section: Methodsmentioning

confidence: 99%

“…The immunoprecipitated proteins were analyzed in 10% polyacrylamide-sodium dodecyl sulfate (SDS) gels by using a discontinuous Trisglycine buffer system (19). For electrofocusing analysis, purified labeled viral proteins were resuspended in electrofocusing sample buffer and separated in a nonequilibrium pH gradient on slab gels as previously described (14).…”

Section: Methodsmentioning

confidence: 99%

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Modification of foot-and-mouth disease virus O1 Caseros after serial passages in the presence of antiviral polyclonal sera

Rojas

Carrillo

Schiappacassi

et al. 1992

J Virol

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Foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability and, like other RNA viruses, presents a high rate of mutation. It has been proposed that selection exerted by antibodies of the host could play a major role in the rapid evolution of FMDV. The present work reports the selection of FMDV antibody-resistant (Nr) populations after serial passages of a cloned FMDV 01 Caseros strain on secondary monolayers of bovine kidney cells in the presence of subneutralizing antiviral polyclonal sera (APS). After a limited number of passages, i.e., 29, under selective pressure, the virus population showed the following characteristics: (i) increased resistance to neutralization by APS (Nr), (ii) altered electrophoretic mobility of its structural viral proteins (VP1), and (iii) alterations at the RNA nucleotide sequence that codes for the major antigenic site of VP1. These acquired characteristics were detected at passage 15 and remained unmodified throughout successive passages. These results document a rapid selection and fixation of specific mutations in response to immunological pressure. In addition, the findings that (i) mutations not related to APS selection were not detected and (ii) after 29 passages at a high multiplicity of infection without immunological pressure, the RNA sequence that codes for VP1 remained unmodified clearly demonstrated that FMDV 01 Caseros presents in vitro a remarkable unexpected genetic stability.

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“…APS were raised in guinea pigs against FMDV 01 Caseros original strain (OpO) as previously described (14).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%