Phosphorus (P) in irrigation runoff may enrich offsite water bodies and streams and be influenced by irrigation water quality and antecedent soil surface conditions. Runoff, soil loss, and P fractions in runoff using reverse osmosis (RO) water or mixed RO and well water (RO/ Tap) were studied in a laboratory sprinkler study to evaluate water source effects on P transport. A top-or subsoil Portneuf silt loam (coarse-silty, mixed, superactive, mesic Durinodic Xeric Haplocalcid), either amended or not amended with manure and/or with cheese whey, with Olsen P from 20 to 141 mg kg-' and lime from 108 to 243 g kr', was placed in L5 x 1.2 X 0.2-m-deep containers with 2.4% slope and irrigated three times from a 3-m height for 15 min, applying 20 mm of water. The first irrigation was on a dry loose surface, the second on a wet surface, and the third on a dry crusted surface. Surface (ca. 2 cm) soil samples, prior to the first irrigation, were analyzed for Olsen P, water-soluble P (P",), and iron-oxide impregnated paperextractable P (FeO-P) analyses. Following each irrigation we determined runoff, sediment, dissolved reactive phosphorus (DRP) in a 0.45-pm filtered sample, and Fe0-P and total P in unfiltered samples. Soil surface conditions had no effect on P runoff relationships. Water source bad no significant effect on the relationship between DRP or FeO-P runoff and soil test P, except for DRP in RO runoff versus water-soluble soil P (r2 = 0.90). Total P in RO runoff versus soil P were not related; but weakly correlated for Rd/ Tap (r z < 0.50). Water source and soil surface conditions had little or no effect on P runoff from this calcareous soil. I N the quest to reduce phosphorus (P) enrichment of water bodies and streams by runoff from agricultural fields, many factors and variables must be considered. Included are soil physical (texture and aggregation) and chemical properties (P content, pH, mineralogy), land management (tillage, P application, slope, erosivity), and offsite transport processes. These factors affect not only susceptibility for erosion and runoff to occur, but also the potential for soil P release to runoff water. Many of these factors and relations among them are discussed by contributors to the volume edited by Tunney et al. (1997). Their discussions center mainly around rainfed agricultural practices on acidic soils largely covered by vegetation.Dissolved reactive phosphorus (DRP) concentrations in soil solution required for plant growth are in the range of 0.2 to 0.3 mg L-1 . Total P concentrations as low as 0.02 mg L-1 may cause eutrophication of lakes and streams (USEPA, 1996). The USEPA (1986) recommended a limit of 0.05 mg total P L-' in streams flowing into lakes, and 0