Furostanol saponins and ecdysones with cytotoxic activity from <i>Helleborus bocconei</i> ssp. <i>intermedius</i>

Rosselli, Sergio; Maggio, Antonella; Bruno, Maurizio; Spadaro, Vivienne; Formisano, Carmen; Irace, Carlo; Maffettone, Carmen; Mascolo, Nicola

doi:10.1002/ptr.2569

Cited by 14 publications

(6 citation statements)

References 22 publications

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“…The plant extract is used as an anti-rheumatic ointment without any previous investigation of the activity of the individual components of hellebore, therefore, the component(s) responsible for the biological activity of Boicil have not been identified yet. However, recent studies on the antitumor, immune and biological activity of Helleborus extracts revealed the multitude of directions in this field of research targeting the elucidation of the biological potential and cellular mechanisms addressed by various components from this plant [6,7,8]. Some of the main components of Helleborus purpurascens are glycosides, thionins (helleborine, hellebrine), saponides, resins, lactones, protoanemonine, and minerals [6,7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

“…The antitumor and immunomodulating activities of hellebore are attributed to the cytotoxicity of this natural peptide [15,16,17,18,19]. Although there are many studies worldwide on hellebore extracts and their biological activities, research in this area continues to be of great interest because the chemical structure and composition of Helleborus purpurascens have not been fully elucidated yet [6,20,21,22,23]. The aim of this study was hence to isolate the amino acids and thionins from an alcoholic extract of Helleborus purpurascens , identify them using spectroscopic and chromatographic techniques and to test their biological activity in terms of antiproliferative effects.…”

Section: Introductionmentioning

confidence: 99%

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Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

et al. 2015

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There is a strong drive worldwide to discover and exploit the therapeutic potential of a large variety of plants. In this work, an alcoholic extract of Helleborus purpurascens (family Ranunculaceae) was investigated for the identification of amino acids and peptides with putative antiproliferative effects. In our work, a separation strategy was developed using solvents of different polarity in order to obtain active compounds. Biochemical components were characterized through spectroscopic (mass spectroscopy) and chromatographic techniques (RP-HPLC and GC-MS). The biological activity of the obtained fractions was investigated in terms of their antiproliferative effects on HeLa cells. Through this study, we report an efficient separation of bioactive compounds (amino acids and peptides) from a plant extract dependent on solvent polarity, affording fractions with unaffected antiproliferative activities. Moreover, the two biologically tested fractions exerted a major antiproliferative effect, thereby suggesting potential anticancer therapeutic activity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

et al. 2015

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“…is represented by a dried rhizome of dark brown to black colour, thickly overgrown by thin, dark grey roots (Hellebori rhizoma et radix), and is being extracted in spring or in autumn. Hellebori rhizoma et radix composed of various active components including bufadienolides (Wissner and Kating, 1974;Muhr et al, 1995), steroidal saponins (Ribár et al, 1986;Vladimirov et al, 1991), lipids (Colombo et al, 1991), ecdysones (Colombo and Tomè, 1993;Rosselli et al, 2009) and alkaloids (Slavík et al, 1987), has long been used in traditional folk veterinary medicine. Because of its irritating influence on skin and mucous membrane, the rhizom of hellebore is, in etnoveterinary medicine, used in "herbal treatment" for a great number of farm animals (horses, pigs, sheep).…”

Section: The Objective Of This Research Was To Study the Effects Thatmentioning

confidence: 99%

The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats

Davidović¹,

Lazarević²,

Joksimovic-Todorovic³

et al. 2010

Acta vet. (Beogr.)

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The objective of this research was to study the effects that the extract of rhizome and root of Helleborus odorus W. et K. (Ranunculaceae) can have on modifications in the parameter values of white blood cells count and degree of phagocytosis by peritoneal macrophages and neutrophil granulocytes in Wistar rats. The trial was conducted on 28 rats divided into 4 groups with 7 animals in each group. To the control group of rats sterile physiological solution in the quantity of 0.25 mL/100 g BW was applied intramuscularly. For the purpose of monitoring the effect of the extract of rhizome and root of hellebore (HE) during a time period, the HE was applied intramuscularly to rats in a dose of 10 mg/100 g BW, while the blood samples for analysis were taken after 24h, 48h and 72h. The consequence of intramuscular application of HE was an increased count of total leukocytes in all trial groups, the most expressed leukocytosis being registered 24h after application of HE. Statistically significant higher value in the count and percent of neutrophil granulocytes in the blood was recorded 24h after treatment in relation to the control and two other trial groups (p<0.001), among which a statistical significance was not established. The extract of hellebore rhizome and root has led to lymphopenia, resulting in the increase of the neutrophil/limphocyte index in the trial groups 24h and 48h after treatment. The application of HE had no significant effect on the count of monocytes in treated animals. The applied extract has caused a significant increase in the degree of phagocytosis by residing peritoneal macrophages and neutrophil granulocytes in blood

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“…intermedius proved to have cytotoxic activity against rat C6 glioma cells. Three isolated compounds, a hydrolyzed form of helleboroside B, a furospirostanol saponin and polypodyne B showed significant cytotoxic activity, recording a 60-70% inhibition of the cell growth [73].…”

Section: Cytotoxicity and Anticancer Propertiesmentioning

confidence: 99%

Natural compounds with important medical potential found in Helleborus sp.

Maior

Dobrotă²

2013

Open Life Sciences

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Helleborus (family Ranunculaceae) are well-known as ornamental plants, but less known for their therapeutic benefits. Over the past few years, Helleborus sp. has become a subject of interest for phytochemistry, pharmacology and other medical research areas. On the basis of their usefulness in traditional medicine, it was assumed that their biochemical profile could be a source of metabolites with the potential to overcome critical medical issues. There are studies involving natural extracts from these species which demonstrate that Helleborus plants are a valuable source of chemical compounds with great medical potential. Some phytochemicals produced by these species have been separated and identified a few decades ago: hellebrin, deglucohellebrin, 20-hydroxyecdysone and protoanemonin. Lately, many other active compounds have been reported and considered as promising remedies for severe diseases such as cancer, ulcer, diabetes and also for common medical problems such as toothache, eczema, low immunity and arthritis. This paper is an overview of the Helleborus genus focusing on some recentlydiscovered compounds and their potential for finding new drugs and useful biochemicals derived from these species.

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Furostanol saponins and ecdysones with cytotoxic activity from Helleborus bocconei ssp. intermedius

Cited by 14 publications

References 22 publications

Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in wistar rats

Natural compounds with important medical potential found in Helleborus sp.

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