Furildioxime method for nickel analysis in serum and urine by electrothermal atomic absorption spectrometry.

Abstract

We describe a method of analysis for nickel in serum and urine. After the sample (1 ml) has been subjected to acid digestion, Ni(II) is extracted into methylisobutylketone as a furildioximate complex, and is measured by electrothermal atomic absorption spectrometry. The method is more sensitive and more convenient than previous methods, and is less subject to nickel contamination because the digestion and extraction steps are performed in a single tube. Analytical recovery of Ni(II) (10 or 20 microng/liter) added to 20 specimens of serum or urine averaged 101% (range, 94 to 107). The following values (mean +/- SD) were obtained for nickel concentrations in specimens of serum and urine from 19 healthy adults living in central Connecticut: serum = 3.1 +/- 1.6 microng/liter; urine = 2.7 +/- 1.6 microng/liter. The method provides a practical means for monitoring occupational exposures of workers to nickel compounds.

Evaluation of toxic metals in biological samples (scalp hair, blood and urine) of steel mill workers by electrothermal atomic absorption spectrometry

“…The range of nickel in the blood, urine and scalp hair samples of normal controls were found to be between 0.81Á3.12, 2.26Á10.29 and 2.44Á7.96 mg/g, respectively. The mean value of nickel in urine was 6.319/1.91 mg/L, in good agreement with the results of some investigations (Mikac-Devic et al, 1977;Andersen et al, 1978;Chmielnicka, 1986). The levels of nickel in blood and urine were 3.319/1.56 and 4.079/1.84, 7.629/1.78 and 9.479/2.95 mg/L, while 9.029/2.64, 13.769/4.48 mg/g were observed in scalp hair of QCW and PW, respectively.…”

“…A number of heavy metals, as well as dust, fumes and gases, are found in these working environments. The exposure may have both acute and long term health effects (Mikac-Devic et al, 1977).…”

Determination of nickel by differential pulse polarography at a dropping mercury electrode

“…The detection limit of 2 ppb (at a confidence interval of 95%) and observed standard deviations of the same magnitude for the DMG-sensitized procedure are comparable to those possible with modern electrothermal AA analysis of Ni (1,4,47,48). Detection limits and precision are virtually limited by the ability to avoid contamination.…”

Evaluation of arsenic, cobalt, copper and manganese in biological Samples of Steel mill workers by electrothermal atomic absorption Spectrometry

“…A number of elements as well as dust fumes and gases are found in these working environments. The exposure may have both acute and long term health effects (Mikac-Devic, et al, 1977).…”