Furan toxicity on testes and protective role of lycopene in diabetic rats

Abstract

Material and Methods35 male adult Wistar albino rats weighing 300 to 320 g were obtained from the Experimental Animal Laboratory of Çukurova University. The rats were inserted into special plasObjective: Furan (C 4 H 4 O) is a heat-induced food contaminant that is utilized as an industrial chemical agent. Lycopene is a natural substance that is produced by plants and tomatoes. We aimed to evaluate the toxicity of furan on testes and the protective effect of lycopene in diabetic rats. Material and Methods:Male Wistar albino rats were divided into five groups: Group 1 (control group) received 1 mL/kg corn oil. Group 2 (diabetic control group) received 55 mg/kg STZ and 1 mL/kg corn oil. Group 3 (diabetic lycopene group) received 55 mg/kg STZ and 4 mg/kg lycopene. Group 4 (diabetic furan group) received 55 mg/kg STZ and 40 mg/kg furan. Group 5 (diabetic furan + lycopene group) received 55 mg/kg STZ, 40 mg/kg furan, and 4 mg/kg lycopene. After 28 days, the testes were extirpated in all groups. In the testicular tissue samples, the level of malondialdehyde (MDA) and the activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and reducted glutathione (GST) were studied. Serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured. Histopathologic examination was performed by light microscope. Results:The MDA level and the activities of CAT, GPx, SOD, and GST were found to be higher in the furan group than in the control and diabetic control groups (p<0.05). The MDA level and the activities of CAT, GPx, SOD, and GST were significantly lower in the furan + lycopene group than in the furan group (p<0.05). Conclusion:The low blood testosterone level in the rats who received furan suggested the presence of endocrinological defects and cellular degenerative changes. Lycopene may be effective to reverse furan toxicity in diabetic rat testes. (J Turk Ger Gynecol Assoc 2016; 17: 191-6) Keywords: Furan, rat, testis, toxicity, lycopene, diabetes mellitus Received: 23 August, 2016 Accepted: 15 October, 2016 Furan toxicity on testes and protective role of lycopene in diabetic rats Özlem Kara, Hatice Baş, Dilek Pandır Department of Biology, Bozok University School of Arts and Science, Yozgat, Turkey Abstract tic cages and were fed with free access to both water and a standard pellet diet. The study was approved by the Institutional Ethics Committee of Çukurova University. ChemicalsIn this experimental study, three substances, including streptozotosin (STZ), furan, and lycopene, were administered; all chemicals used were obtained from Sigma. Furan and lycopene were dissolved in corn oil before being administered to the animals. Implementation plan for animalsChemicals were administered to the rats between 9:00 A.M. and 11:00 A.M. 55 mg/kg STZ was given to each rat by intraperitoneal (ip) injection as a single dose. Two days later, the blood glycose levels of the rats were measured. Rats with a glucose range of 300 mg/dL were accepted as diabetic. F...

Effects of endocrine disruptor furan on reproductive physiology of Sprague Dawley rats: An F1 Extended One-Generation Reproductive Toxicity Study (EOGRTS)

“…Subchronic furan and acrylamide exposure resulted in reduced seminal vesicle and testicular weight, along with reduced serum level of T, LH, FSH, and prolactin. 6,19,45 The bw is the most sensitive indicator of adverse effect of xenobiotics. It is notable that observing the bw is important for interpretation of reproductive effects of toxins.…”

“…6,11,12 Adverse effect of furan has been tested for a variety of reproductive parameters, and prominent effects were reported in the reproductive systems of males and females. 11,[18][19][20] However, a study was conducted on Taiwanese boys whose mothers used contaminated (with polychlorinated biphenyls (PCBs) and furan) rice oil that resulted in smaller size of penis in comparison with age-matched control boys. 21 Similarly, Guo et al reported that sperms of prenatally exposed young men with PCBs and furan have abnormal sperm morphology, reduced sperm motility, and also reduced ability of sperm to enter hamster oocytes.…”

Testicular toxicity of gentamicin in adult rats: Ameliorative effect of lycopene

“…Groups III and IV received gentamicin for 10 consecutive days, starting from the sixth day of the experiment till day 15. The doses of gentamicin 3,4 and lycopene [19][20][21] in this study were selected on the basis of the previous studies. The experiment was continued for 15 consecutive days.…”

Furan-induced cardiotoxicity in diabetic rats and protective role of lycopene

“…Cong et al (2015) and Chen et al (2016) who find eosinophilia in myocardial fibers and edema in connective tissue. Also, chemicals can cause histopathological changes in tissues, such as: liver (Bas, Pandır, & Kalender, 2016), kidneys (Pandir et al, 2016), and testes (Kara, Baş, & Pandır, 2016). Heart is one of the organs that is affected by chemical treatment.…”