Fungos: Biodiversidade E Biotecnologia, Um Breve Panorama

Neto, Benedito Rodrigues da Silva

doi:10.22533/at.ed.3071918101

Cited by 1 publication

(1 citation statement)

References 29 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Much of the research that can revolutionize the processes of generating products, food and energy in a way that is beneficial to the planet, is being directed to groups of microorganisms that have long gone unnoticed by humanity, although already used for millennia of years. Among these, living in the most diverse aquatic and terrestrial environments from the tropics to the Arctic and Antarctic regions are those belonging to the phylum of the Fungi which besides having a great physiological variety and dimensions, from visible to the naked eye to visible only through the microscope, can make possible impacts positive to sustainable development when used appropriately in the generation of useful products, such as: in the synthesis of insecticidal substances that help in pest control, in the bioremediation of environments degraded by chemical pollutants and by the degradation of cellulolytic compounds and of the lignin itself present in plants (Maia, 2010;Silva & Coelho, 2006).…”

Section: Introductionmentioning

confidence: 99%

Biotechnology: use of available carbon sources on the planet to generate alternatives energy

Junior,

Vicente,

Marasca

et al. 2024

Preprint

View full text Add to dashboard Cite

Biotechnology has been an essential tool in the search for solutions and in the optimization of bioprocesses associated with issues of human, plant, animal, energy and also the balance of ecosystems on planet Earth. The objective of this research was to present an unconventional substrate (cellulose), in abundance on the planet, to be used as a substitute source of carbon and energy for biotechnology processes, with the possibility of increasing industrial production of biomass and energy. As basis for the research, an extensive literature review and quantitative and qualitative analyzes were carried out. We chose to qualify the yeast Saccharomyces cerevisiae for partial cellulose degradation, through the use of genetic transformation methods to insert a plasmid carrying the cellobiohydrolase cDNA. It was found that the recombinant and biologically active cellobiohydrolase protein was expressed and excreted in haploid and diploid laboratory yeast strains. The analyzes allowed the visualization of cellulolysis halos around colonies of recombinant strains grown in solid YPD medium with 1% microgranular cellulose. The recombinant clones derived from the haploid lineage yielded in average of 1.70 mg ART/mL, while recombinant clones derived from the diploid lineage produced in average of 2.05 mg ART/mL.

show abstract

Section: Introductionmentioning

confidence: 99%