Fungicide multiresidue monitoring in international wines by immunoassays

Esteve‐Turrillas, Francesc A.; Agulló, Consuelo; Abad‐Somovilla, Antonio; Mercader, Josep V.; Abad‐Fuentes, Antonio

doi:10.1016/j.foodchem.2015.10.102

Cited by 35 publications

(24 citation statements)

References 30 publications

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“…[8,[17][18][19][20] Esteve-Turrillas et al have recently published a survey of the occurrence of some fungicides in international wines, by using rapid antibody-based assays. [21] This study shows that contamination of commercial wines with pesticides is an issue of worldwide relevance, with potential implications for consumer health and international trade. Coleman et al have explored the effects of a combination of three commonly used biocides (pyrimethanil, cyprodinil and fludioxonil) on cell viability, mitochondrial health and generation of oxidative stress, alone and in combination.…”

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confidence: 88%

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Photodegradation of cyprodinil under UV–visible irradiation – chemical and toxicological approaches

Nicol

Chayata

Genty

et al. 2016

Rapid Comm Mass Spectrometry

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Structures of photoproducts were characterized after irradiation of a cyprodinil aqueous solution combining LC/HRMS, LC/HRMS/MS and the use of a labeled compound. Their formations imply several photodegradation reactions, namely direct bond cleavages, cyclization, isomerization and hydroxylation. Most of the TPs exhibit a toxicity significantly higher than that of the parent molecule. Copyright © 2016 John Wiley & Sons, Ltd.

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confidence: 88%

“…Esteve‐Turrillas et al . have recently published a survey of the occurrence of some fungicides in international wines, by using rapid antibody‐based assays . This study shows that contamination of commercial wines with pesticides is an issue of worldwide relevance, with potential implications for consumer health and international trade.…”

mentioning

confidence: 97%

Photodegradation of cyprodinil under UV–visible irradiation – chemical and toxicological approaches

Nicol

Chayata

Genty

et al. 2016

Rapid Comm Mass Spectrometry

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“…The obtained results are in accordance to literature data where the maximum anilinopyrimidine concentrations were from 15 to 234 μg L −1 . [5][6][7][8][9][10] The European Union does not establish maximum residue limits (MRL) for processed food beverages, and application of MRL values for the raw commodity -wine grapes in this case -is not probably adequate to establish quality and safety parameters for wine. Ultraviolet-visible spectra and ELISA absorbances were read with a PowerWave HT from BioTek Instruments (Winooski, VT, USA).…”

Section: Validation Studymentioning

confidence: 99%

“…Several studies have appointed the actual transference of fungicide residues from grapes to wine, [2][3][4] being cyprodinil and pyrimethanil among the most common pesticides found in this valuable commodity. [5][6][7][8][9][10] Fungicide residues in wine are usually analyzed by chromatographic multianalyte methods, particularly highperformance liquid chromatography with mass spectrometry detection (HPLC-MS), supported by sample extraction procedures like QuEChERS (acronym for Quick, Easy, Cheap, Effective, Rugged, and Safe). [11][12][13] Moreover, alternative extraction methodologies have also been developed recently for the analysis of anilinopyrimidine residues in wine using liquid-liquid extraction, 14 solid-phase extraction, 15,8,16 solidphase microextraction, 17 dispersive liquid-liquid microextraction, 7 bar adsorptive microextraction, 18 and ultrasound-assisted microextraction.…”

Section: Introductionmentioning

confidence: 99%

A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten

Esteve‐Turrillas

Mercader

Agulló

et al. 2017

Analyst

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The development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil, and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with IC50 values of 0.26, 0.27, and 0.25 μg L −1 for pyrimethanil, cyprodinil, and mepanipyrim, respectively. This immunoassay was fully characterized and applied to the multianalyte determination of anilinopyrimidine fungicides in white and red wines, with a limit of quantification of 1 μg L −1, average recoveries from 93.1% to 114.4%, and relative standard deviations lower than 20%. Commercial wine samples were analyzed and those containing detectable anilinopyrimide residues were verified by a reference chromatographic technique.

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“…La selectividad de los ensayos se evalúa mediante el empleo de distintos compuestos como competidores, lo que permite conocer cuáles son los más adecuados para el objetivo perseguido, ya sea un ensayo específico o genérico. Este último tipo de inmunoensayo resulta especialmente útil para realizar un cribado inicial con un elevado número de muestras en las que se sospecha de la presencia de compuestos pertenecientes a una misma familia [124][125][126]. Para continuar con la optimización y desarrollo de los inmunoensayos se realiza un estudio de caracterización con el fin de evaluar su comportamiento frente a la variación de parámetros fisicoquímicos como la temperatura, el tiempo de incubación, el pH, la fuerza iónica, la presencia de disolventes orgánicos, entre otros, ya que pueden afectar el ~ 56 ~ reconocimiento molecular.…”

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Desarrollo de métodos inmunoquímicos para la determinación de sustancias tóxicas en alimentos y aguas

Cedeño¹,

Eudoro²

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The aim of this doctoral thesis is the study, development and validation of different immunochemical methods for determining chemical contaminants in produce and water, in a way that they may contribute to improving food quality and therefore to assure consumer safety. Spirotetramat is a highly efficient new-generation pesticide, marketed worldwide for use as insecticide in many agricultural crops. It has systemic properties, since short after absorption it translocates through both the xylem and the phloem, thanks to the fact that it is transformed by the plant into the much more polar spirotetramat-enol. Consequently, the residue definition for this insecticide in foods of plant origin with analytical purposes also includes said metabolite. On the other hand, anatoxin-a is a secondary alkaloid with acute neurotoxicity that can be found in fresh water. This toxin is produced by seven different genera of cyanobacteria, and has been detected in lakes and other water resources on all continents. The analysis of substances like spirotetramat and anatoxin-a is currently carried out by chromatographic methods like HPLC-MS. These techniques are highly sensitive and reliable; however, they require highly qualified personnel and expensive, non-portable equipment. Nowadays, the immunochemical methods, such as the ELISA (Enzyme-Linked ImmunoSorbent Assay) or the LFIA (Lateral Flow ImmunoAssay), constitute excellent complementary analytical options to instrumental strategies, since they are fast and inexpensive analytical methods, and are also very versatile so they can be adapted to particular analytical needs, such as screening assays for large numbers of samples or portable tests with visual reading of the results. The antibody affinity and specificity from a collection of spirotetramat and anatoxin-a immunoreagents was characterized in order to select conjugate/antibody pairs suitable for the development of competitive ELISA and LFIA tests. The assay conditions were optimized, and a study of the influence of different physicochemical factors over the analytical parameters of the selected immunoassays was carried out. Subsequently, the influence of the food matrix, particularly grape, grape juice and wine, as well as water from different sources, over the assay signal and sensitivity was evaluated. The different affinity of the available antibodies towards spirotetramat and spirotetramatenol led us to optimize the sample treatment procedure, so a hydrolysis step to transform spirotetramat into spirotetramat-enol in a controlled, rapid and quantitative way, was included. Thus, it was possible to provide results in the form of the sum of the concentration of both compounds in the sample, as required by current legislation. In addition, a procedure ~ 13 ~ using QuEChERS technology was developed to extract residues of this insecticide from grape samples, and polyvinylpolypyrrolidone was used to reduce interferences from wines and juices. In the case of waters, a simple filtration was applied to remove suspended particles. Mi...

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Fungicide multiresidue monitoring in international wines by immunoassays

Cited by 35 publications

References 30 publications

Photodegradation of cyprodinil under UV–visible irradiation – chemical and toxicological approaches

Photodegradation of cyprodinil under UV–visible irradiation – chemical and toxicological approaches

A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten

Desarrollo de métodos inmunoquímicos para la determinación de sustancias tóxicas en alimentos y aguas

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