Fungicide control of Gnomoniopsis smithogilvyi, causal agent of chestnut rot in Australia

Silva-Campos, Matias; Islam, Md. Touhidul; Cahill, David M.

doi:10.1007/s13313-022-00879-4

Cited by 8 publications

(11 citation statements)

References 28 publications

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“…Moreover, those were described as showing cross-resistance even for fungicides with a different mode of action , which might be the reason for their high sequence read abundances in the insecticide Steward ® -treated bees (Figure 4A). Similar to the cuticular fungal community composition, the diversity of functional composition was increased due to the fungicide Difcor ® , insecticide Steward ® , and PPP mix A treatments, which were already observed for the intestinal microbiome of bees (Silva-Campos et al, 2022).…”

Section: Discussionsupporting

confidence: 56%

Hazardous pollutants in agricultural soil and environment

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Discussionsupporting

confidence: 56%

Hazardous pollutants in agricultural soil and environment

2024

Frontiers Research Topics

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“…Moreover, those were described as showing cross-resistance even for fungicides with a different mode of action (Yang et al, 2019), which might be the reason for their high sequence read abundances in the insecticide Steward ® -treated bees (Figure 4A). Similar to the cuticular fungal community composition, the diversity of functional composition was increased due to the fungicide Difcor ® , insecticide Steward ® , and PPP mix A treatments, which were already observed for the intestinal microbiome of bees (Silva-Campos et al, 2022).…”

Section: Discussionsupporting

confidence: 56%

“…Similarly, the fungicide Difcor® significantly reduced the fungal gene copy numbers ( Supplementary Figure 1B ), and members of the genus Amphiporthe were significantly associated with this treatment. However, a recent study found that members of the same family, Valsaceae, were described as sensitive toward difenoconazole ( Silva-Campos et al, 2022 ), indicating an ambivalent biocide response in this family. Furthermore, Steward® significantly changed cuticular fungal community composition and function ( Figures 3B , 4A ; Supplementary Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Fungicides and insecticides can alter the microbial community on the cuticle of honey bees

Reiß,

Schuhmann,

Sohl

et al. 2023

Front. Microbiol.

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Honey bees are crucial for our ecosystems as pollinators, but the intensive use of plant protection products (PPPs) in agriculture poses a risk for them. PPPs do not only affect target organisms but also affect non-targets, such as the honey bee Apis mellifera and their microbiome. This study is the first of its kind, aiming to characterize the effect of PPPs on the microbiome of the cuticle of honey bees. We chose PPPs, which have frequently been detected in bee bread, and studied their effects on the cuticular microbial community and function of the bees. The effects of the fungicide Difcor® (difenoconazole), the insecticide Steward® (indoxacarb), the combination of both (mix A) and the fungicide Cantus® Gold (boscalid and dimoxystrobin), the insecticide Mospilan® (acetamiprid), and the combination of both (mix B) were tested. Bacterial 16S rRNA gene and fungal transcribed spacer region gene-based amplicon sequencing and quantification of gene copy numbers were carried out after nucleic acid extraction from the cuticle of honey bees. The treatment with Steward® significantly affected fungal community composition and function. The fungal gene copy numbers were lower on the cuticle of bees treated with Difcor®, Steward®, and PPP mix A in comparison with the controls. However, bacterial and fungal gene copy numbers were increased in bees treated with Cantus® Gold, Mospilan®, or PPP mix B compared to the controls. The bacterial cuticular community composition of bees treated with Cantus® Gold, Mospilan®, and PPP mix B differed significantly from the control. In addition, Mospilan® on its own significantly changed the bacterial functional community composition. Cantus® Gold significantly affected fungal gene copy numbers, community, and functional composition. Our results demonstrate that PPPs show adverse effects on the cuticular microbiome of honey bees and suggest that PPP mixtures can cause stronger effects on the cuticular community than a PPP alone. The cuticular community composition was more diverse after the PPP mix treatments. This may have far-reaching consequences for the health of honey bees.

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“…Thus, growers would have timely information that would enable them to design and implement effective control strategies for chestnut rot. For example, in our recent report on the use of fungicides to control G. smithogilvyi in the field [18], we used this mPCR technique, across a growing season, to monitor the level of nut infection after fungicide applications.…”

Section: Discussionmentioning

confidence: 99%

“…Implementing control strategies, such as the use of fungicides [18], for plant diseases is time-dependent and relies on the detection and identification of the causative agent precisely and rapidly [19,20]. Currently, numerous plant pathogens, including ascomycetes [21], basidiomycetes [22,23] and oomycetes [24], are detected quickly and accurately using PCR-based techniques [25].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Accurate Detection of Gnomoniopsis smithogilvyi the Causal Agent of Chestnut Rot, through an Internally Controlled Multiplex PCR Assay

2022

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The fungus Gnomoniopsis smithogilvyi is a significant threat to the production of sweet chestnut (Castanea sativa) nuts in Australia and worldwide. The pathogen causes nut rot, which leads to substantial production losses. Early and accurate diagnosis of the disease is essential to delineate and implement control strategies. A specific and sensitive multiplex PCR was developed based on the amplification of three barcode sequences of G. smithogilvyi. The assay reliability was enhanced by including the amplification of a host gene as an internal control. Primers were thoroughly evaluated in silico before assessing them in vitro. Primer annealing temperature and concentration were optimised to enhance the assay sensitivity and specificity. The assay detection limit ranged between 0.1 and 1.0 pg (5 and 50 fg/μL) of genomic DNA per reaction. No cross-reactivity was observed with genomic DNA from closely and distantly related fungal species. We also characterised Australian G. smithogilvyi isolates phenotypically and genotypically and found significant differences in morphologic and virulence traits of the isolates. An understanding of the virulence of G. smithogilvyi and the availability of a reliable and accurate diagnostic technique will enable earlier detection of the pathogen, which will contribute to effective control strategies for the disease.

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Fungicide control of Gnomoniopsis smithogilvyi, causal agent of chestnut rot in Australia

Cited by 8 publications

References 28 publications

Hazardous pollutants in agricultural soil and environment

Hazardous pollutants in agricultural soil and environment

Fungicides and insecticides can alter the microbial community on the cuticle of honey bees

Rapid and Accurate Detection of Gnomoniopsis smithogilvyi the Causal Agent of Chestnut Rot, through an Internally Controlled Multiplex PCR Assay

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