Fungicidal effect of Lippia alba essential oil on a white-rot fungus

Abstract

Lippia alba is a plant that has antifungal activity against Aspergillus, Penicillium and Trichoderma genera as well as against human pathogenic microorganisms; however, there are no records on its effect on basidiomycetes which are responsible for white rot of wood. The objective of this study was to evaluate the antifungal activity of L. alba for the control of the white-rot fungus Pleurotus ostreatus. From L. alba leaves, essential oil (EO) was extracted by hydrodistillation, alcoholic extract (AE) was obtained through alcoholic maceration, and aqueous extract (QE) from aqueous infusion. Each extract was added to several culture media to evaluate the fungicidal effect on P. ostreatus. AE and QE do not have fungicidal activity. P. ostreatus does not survive EO when concentrations are higher than 1,0 mL L -1 in malt extract liquid culture medium, or higher than 9,0 mL L -1 in particulate sawdust solid culture medium. The physical state of the cultivation medium affects the fungicidal action of EO. In surfaces subject to greater volatility, the minimum fungicidal concentration of the EO is 25,0 mL L -1 . Altogether, L. alba EO is a potential alternative of biological control of basidiomycetes of white rottenness in wood.

Essential oil composition, antifungal activity and leaf anatomy of Lippia alba (Verbenaceae) from Brazilian Chaco

“…The antifungal activity of different chemotypes of L. alba essential oil against pathogenic fungi was also recently investigated (Rao et al, 2000;Mesa-Arango et al, 2009;Glamočlija et al, 2011;Geromini et al, 2015;Pandey et al, 2016). Results similar to those of the present study were obtained from leaf essential oils at a concentration of 1 μg/mL against Aspergillus sp CPV34.…”

Natural durability of Cedrus atlantica wood related to the bioactivity of its essential oil against wood decaying fungi

“…Furthermore, separate alcoholic and aqueous extracts of Lipia alba essential oil, did not have any fungicidal activity at any concentration in culture medium. However L. alba essential oil presented fungicide activity against the white-rot fungus, Pleurotus ostreatus (Geromini et al 2015).…”

Efficient control of conidium germination, mycelial growth and early blight in tomato in vitro with essential oils under farm conditions

“…The best results for MIC 50 and 90 with minor dose necessary to control was essential oil from C. citratus at 341, 32 and 1.822,10 µL L -1 respectively (Table 3). These results are corroborated by Glamočlija et al (2011) andGeromini et al (2015) on the effectiveness of Geranial and Neral aldehyde mixture that constitutes citral, a potent bioactive substance that fights against phytopathogens, especially fungi and post-harvest and storage diseases. Farming under agroecological system is possible with the use of these essential oils.…”