Fungi inhabiting fine roots of Pinus heldreichii in the Montenegrin montane forests

Lazarević, Jelena; Menkis, Audrius

doi:10.1007/s13199-017-0504-5

Cited by 9 publications

(13 citation statements)

References 31 publications

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“…This experimental constraint is consolidated by the fact that no relevant information on the abundances and diversity of this conifer’s root fungal endophyte community is currently available in the literature. The overall community composition observed in this study greatly differs from other studies of conifer roots [19,20,24,60,61]. This study encountered only a single PAC species (from P .…”

Section: Discussioncontrasting

confidence: 62%

New R-Based Methodology to Optimize the Identification of Root Endophytes against Heterobasidion parviporum

2019

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Many root fungal endophytes inhabiting forest trees have potential impact on the health and disease progression of certain tree species. Hence, the screening of root endophytes for their biocontrol abilities is relevant for their potential to protect their hosts against invaders. The aim of this research is to screen for the potential inhibitory effects of selected conifer root endophytes during interaction, in vitro, with the root rot pathogen, Heterobasidion parviporum. Here, we introduce a guideline that facilitates the use of root fungal endophytes as biocontrol agents. We isolated fungal root endophytes from eight different conifers. These root fungal endophytes were evaluated for their antagonism against the root rot pathogen, H. parviporum, by means of paired-culture antagonism assays. We determined the antagonism of the isolated root fungal endophytes to elucidate potential biocontrol applications. For the analysis, a software package in R was developed. Endophyte candidates with antagonistic potential were identified.

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Section: Discussioncontrasting

confidence: 62%

New R-Based Methodology to Optimize the Identification of Root Endophytes against Heterobasidion parviporum

2019

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“…While Capronia pilosella is cited as being a dark septate fungus (Jumpponen and Trappe, 1998), most sequences in our study aligned more closely (95%) to Capronia peltigerae, which is a lichenicolous fungus (Untereiner et al, 2011). While the Exophiala genus also contains non-endophytic soil fungi, our sequences closely aligned (97%) with an endophyte isolated from fine pine roots in the mountains of Montenegro (Lazarevi c and Menkis, 2017). Unfortunately, there are no studies of how targeted inoculations with these taxa affect plant fitness, which is an avenue for future research.…”

Section: Dse and Amf Community Composition (H4)mentioning

confidence: 54%

“…Studying the landscape distribution of fungi in cold environments, including over plant density and snowpack gradients which are changing with climate warming, can help us understand the potential effects of climate change on plant-fungal interactions. Interestingly, Kyt€ oviita and Ruotsalainen (2007) found that the benefits of an arbuscular mycorrhizal fungus increased at warmer temperatures, suggesting that some arctic or alpine fungi can have positive responses to warming.…”

Section: Introductionmentioning

confidence: 99%

Patterns of root colonization by arbuscular mycorrhizal fungi and dark septate endophytes across a mostly-unvegetated, high-elevation landscape

et al. 2018

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Arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) are two fungal groups that colonize plant roots and can benefit plant growth, but little is known about their landscape distributions. We performed sequencing and microscopy on a variety of plants across a high-elevation landscape featuring plant density, snowpack, and nutrient gradients. Percent colonization by both AMF and DSE varied significantly among plant species, and DSE colonized forbs and grasses more than sedges. AMF were more abundant in roots at lower elevation areas with lower snowpack and lower phosphorus and nitrogen content, suggesting increased hyphal recruitment by plants to aid in nutrient uptake. DSE colonization was highest in areas with less snowpack and higher inorganic nitrogen levels, suggesting an important role for these fungi in mineralizing organic nitrogen. Both of these groups of fungi are likely to be important for plant fitness and establishment in areas limited by phosphorus and nitrogen.

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“…In agreement with this, this mycorrhizal symbiont was commonly reported in association with the roots of forest nursery seedlings [ 90 , 91 ]. Besides this, it was shown that Wilcoxina fungi can reduce the negative effect of salt stress on the plants [ 92 ] and support tree growth in high-altitude marginal habitats [ 93 ]. Its common occurrence in forest nurseries and on different hosts raises the question of its potential effect on seedling performance in forest nurseries, but such information is scarce.…”

Section: Discussionmentioning

confidence: 99%

DNA-Metabarcoding of Belowground Fungal Communities in Bare-Root Forest Nurseries: Focus on Different Tree Species

et al. 2021

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The production of tree seedlings in forest nurseries and their use in the replanting of clear-cut forest sites is a common practice in the temperate and boreal forests of Europe. Although conifers dominate on replanted sites, in recent years, deciduous tree species have received more attention due to their often-higher resilience to abiotic and biotic stress factors. The aim of the present study was to assess the belowground fungal communities of bare-root cultivated seedlings of Alnus glutinosa, Betula pendula, Pinus sylvestris, Picea abies and Quercus robur in order to gain a better understanding of the associated fungi and oomycetes, and their potential effects on the seedling performance in forest nurseries and after outplanting. The study sites were at the seven largest bare-root forest nurseries in Lithuania. The sampling included the roots and adjacent soil of 2–3 year old healthy-looking seedlings. Following the isolation of the DNA from the individual root and soil samples, these were amplified using ITS rRNA as a marker, and subjected to high-throughput PacBio sequencing. The results showed the presence of 161,302 high-quality sequences, representing 2003 fungal and oomycete taxa. The most common fungi were Malassezia restricta (6.7% of all of the high-quality sequences), Wilcoxina mikolae (5.0%), Pustularia sp. 3993_4 (4.6%), and Fusarium oxysporum (3.5%). The most common oomycetes were Pythium ultimum var. ultimum (0.6%), Pythium heterothallicum (0.3%), Pythium spiculum (0.3%), and Pythium sylvaticum (0.2%). The coniferous tree species (P. abies and P. sylvestris) generally showed a higher richness of fungal taxa and a rather distinct fungal community composition compared to the deciduous tree species (A. glutinosa, B. pendula, and Q. robur). The results demonstrated that the seedling roots and the rhizosphere soil in forest nurseries support a high richness of fungal taxa. The seedling roots were primarily inhabited by saprotrophic and mycorrhizal fungi, while fungal pathogens and oomycetes were less abundant, showing that the cultivation practices used in forest nurseries secured both the production of high-quality planting stock and disease control.

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Fungi inhabiting fine roots of Pinus heldreichii in the Montenegrin montane forests

Cited by 9 publications

References 31 publications

New R-Based Methodology to Optimize the Identification of Root Endophytes against Heterobasidion parviporum

New R-Based Methodology to Optimize the Identification of Root Endophytes against Heterobasidion parviporum

Patterns of root colonization by arbuscular mycorrhizal fungi and dark septate endophytes across a mostly-unvegetated, high-elevation landscape

DNA-Metabarcoding of Belowground Fungal Communities in Bare-Root Forest Nurseries: Focus on Different Tree Species

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