Fungal survival under temperature stress: a proteomic perspective

Abstract

Background Increases in knowledge of climate change generally, and its impact on agricultural industries specifically, have led to a greater research effort aimed at improving understanding of the role of fungi in various fields. Fungi play a key role in soil ecosystems as the primary agent of decomposition, recycling of organic nutrients. Fungi also include important pathogens of plants, insects, bacteria, domestic animals and humans, thus highlighting their importance in many contexts. Temperature directly affects fungal growth and protein dynamics, which ultimately will cascade through to affect crop performance. To study changes in the global protein complement of fungi, proteomic approaches have been used to examine links between temperature stress and fungal proteomic profiles. Survey methodology and objectives A traditional rather than a systematic review approach was taken to focus on fungal responses to temperature stress elucidated using proteomic approaches. The effects of temperature stress on fungal metabolic pathways and, in particular, heat shock proteins (HSPs) are discussed. The objective of this review is to provide an overview of the effects of temperature stress on fungal proteomes. Concluding remarks Elucidating fungal proteomic response under temperature stress is useful in the context of increasing understanding of fungal sensitivity and resilience to the challenges posed by contemporary climate change processes. Although useful, a more thorough work is needed such as combining data from multiple -omics platforms in order to develop deeper understanding of the factor influencing and controlling cell physiology. This information can be beneficial to identify potential biomarkers for monitoring environmental changes in soil, including the agricultural ecosystems vital to human society and economy.

Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

“…Chemicals and physical parameters affect microbial growth and, in the case of yeasts, the availability of nutrients, as well as temperature and pH, can affect the development of cells. In the presented investigation, the temperature played a key role in the growth ability of Z. obscura LS31012019 which reached its optimum at 25 °C, thus showing the typical feature of mesophilic fungi, which have T opt of 25 − 30 °C and generally tolerate a wider range of temperatures and related stress (Abu Bakar et al 2020 ). Fungal adaptation to variable temperatures implicates alterations in the utilization of many metabolic pathways to compensate for the amount of energy needed in delivering the stress response.…”

AGC/AKT Protein Kinase SCH9 Is Critical to Pathogenic Development and Overwintering Survival in Magnaporthe oryzae

“…Furthermore, KEGG enrichment analysis shows the deletion of MoSch9 repressed the production of secondary metabolites that are the key factors in the long-term survivability of any living organism and act as a signaling molecule during various stress conditions [ 78 , 79 , 80 ]. Similarly, the KEGG enrichment analysis and Gene Ontology (GO) in ΔMoSch9 strains at 10 °C revealed that a large number of proteins involved in energy metabolism pathways, in ABC transport, in part of cell wall and membrane, and cellular homeostasis were downregulatedMetabolic pathways are one of the main components that are crucial for energy regulation of the cell and hence for cell survival [ 81 ], and MoSch9 deletion resulted in the disruption of carbohydrate metabolic pathways, which is in accordance with the mutant’s compromised growth during cold stress. In addition, fungal ABC transporters have been previously reported to work as an efflux pump to move natural toxins or cytotoxic secondary metabolites [ 34 , 82 ].…”

Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature

“…First, C. thermophilum mycelium was ground by liquid nitrogen, followed by charging the powders into a centrifuge tube (5 mL) and sonicating thrice on ice with an ultrasonic processor (Scientz, Ningbo, China) in a lysis buffer [including 1% Triton X-100, 10 mM dithiothreitol (DTT), 1% protease inhibitor cocktail, 50 μM PR-619, 3 μM TSA, 50 mM NAM, and 2 mM EDTA] ( Abu Bakar et al, 2020 ). Upon adding an equivalent volume of Tris-saturated phenol (pH 8.0), the resulting mixture was vortexed for 5 min.…”

“…High-temperature adaptation has attracted widespread attention. The latest review gave an overview and summary of fungal proteomics under temperature stress ( Abu Bakar et al, 2020 ). The 2D protein gels of Friedmanniomyces endolithicus shows that the amount of protein decreases under high-temperature pressure, indicating a lack of heat shock response ( Tesei et al, 2012 ).…”