Fungal Secondary Metabolism Research: Past, Present and Future

Campbell, I.

doi:10.1021/np50025a003

Cited by 21 publications

(10 citation statements)

References 22 publications

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“…Coker and Couch, cultures, strain DI-15, isolated by Grenville et al [1986] were grown on modified Melin-Norkrans (MMN) agar [Marx, 1969]. To provide material for experiments, a modification of the cellophane sandwich technique of Campbell [1983] was used. The inoculum was grown sandwiched between two discs of sterile cellophane placed on MMN agar according to Cole et al [1997] for 8 -14 days at 23°C in the dark.…”

Section: Fungal Culturesmentioning

confidence: 99%

Regulators of GTP‐binding proteins cause morphological changes in the vacuole system of the filamentous fungus, Pisolithus tinctorius

Hyde

Davies

Cole

et al. 2002

Cell Motil. Cytoskeleton

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Tubule formation is a widespread feature of the endomembrane system of eukaryotic cells, serving as an alternative to the better-known transport process of vesicular shuttling. In filamentous fungi, tubule formation by vacuoles is particularly pronounced, but little is known of its regulation. Using the hyphae of the basidiomycete Pisolithus tinctorius as our test system, we have investigated the effects of four drugs whose modulation, in animal cells, of the tubule/vesicle equilibrium is believed to be due to the altered activity of a GTP-binding protein (GTP gamma S, GDP beta S, aluminium fluoride, and Brefeldin A). In Pisolithus tinctorius, GTP gamma S, a non-hydrolysable form of GTP, strongly promoted vacuolar tubule formation in the tip cell and next four cells. The effects of GTP gamma S could be antagonised by pre-treatment of hyphae with GDP beta S, a non-phosphorylatable form of GDP. These results support the idea that a GTP-binding protein plays a regulatory role in vacuolar tubule formation. This could be a dynamin-like GTP-ase, since GTP gamma S-stimulated tubule formation has only been reported previously in cases where a dynamin is involved. Treatment with aluminium fluoride stimulated vacuolar tubule formation at a distance from the tip cell, but NaF controls indicated that this was not a GTP-binding-protein specific effect. Brefeldin A antagonised GTP gamma S, and inhibited tubule formation in the tip cell. Given that Brefeldin A also affects the ER and Golgi bodies of Pisolithus tinctorius, as shown previously, it is not clear yet whether the effects of Brefeldin A on the vacuole system are direct or indirect.

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Section: Fungal Culturesmentioning

confidence: 99%

Regulators of GTP‐binding proteins cause morphological changes in the vacuole system of the filamentous fungus, Pisolithus tinctorius

Hyde

Davies

Cole

et al. 2002

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…Coker and Couch, strain DI-15, originally isolated by Grenville and co-workers (1986), were grown on modified Melin-Norkrans (MMN) agar (Marx, 1969) using a cellophane-sandwich technique (Campbell, 1983) modified as described previously (Cole et al, 1997). Cultures were kept in the dark at 23°C for 8 -14 days.…”

Section: Fungal Culturesmentioning

confidence: 99%

Brefeldin A Affects Growth, Endoplasmic Reticulum, Golgi Bodies, Tubular Vacuole System, and Secretory Pathway in Pisolithus tinctorius

Cole

Davies

Hyde

et al. 2000

Fungal Genetics and Biology

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“…The products of secondary metabolism (idiolytes), can occur uniquely in a single strain or species, in closely-related members of a single genus, or may be found sporadically in a limited number of evolutionarily-unrelated species (Campbell, 1983). Secondary metabolites usually accumulate in particular organs or tissues, mainly in plants and microorganisms (Haslam, 1986), and include a wide range of chemical structures.…”

Section: Secondary Metabolism: An Introductionmentioning

confidence: 99%

“…Possible roles for these and their significance are discussed later in this section. Secondary metabolites have been referred to as idiolytes, shunt metabolites, special metabolites or 'chemists' compounds' (Campbell, 1983).…”

Section: Secondary Metabolism: An Introductionmentioning

confidence: 99%

“…The focus then moved as to how the organism produced a secondary metabolite from simple precursors, which supplied carbon, hydrogen, oxygen and nitrogen, to the metabolite in question. As a result of such research, three main groups of secondary metabolites were established: polyketides (derived from repeating acetate units), terpene derivatives (via the isoprenoid pathway), and amino acid derivatives (for example, from aromatic amino acids) (Campbell, 1983).…”

Section: Secondary Metabolism: An Introductionmentioning

confidence: 99%

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