Fungal protein and ethanol from lignocelluloses using Rhizopus pellets under simultaneous saccharification, filtration and fermentation (SSFF)

Abstract

Please cite this article as: FazeliNejad S., Ferreira J.A., Brandberg T., Lennartsson P.R., Taherzadeh M.J. Fungal protein and ethanol from lignocelluloses using Rhizopus pellets under simultaneous saccharification, filtration and fermentation (SSFF). Biofuel Research Journal 9 (2016) SSFF can be used for production of ethanol and biomass from wheat straw.Glucose present in the feed controlled the assimilation of xylose and acetic acid.The fungal growth rate was found not to be influenced by the feed composition.Rhizopus biomass yields of up 0.34 g/g and ethanol yields of 0.40 g/g were obtained. The economic viability of the 2 nd generation bioethanol production process cannot rely on a single product but on a biorefinery built around it. In this work, ethanol and fungal biomass (animal feed) were produced from acid-pretreated wheat straw slurry under an innovative simultaneous saccharification, fermentation, and filtration (SSFF) strategy. A membrane unit separated the solids from the liquid and the latter was converted to biomass or to both biomass and ethanol in the fermentation reactor containing Rhizopus sp. pellets. Biomass yields of up to 0.34 g/g based on the consumed monomeric sugars and acetic acid were achieved. A surplus of glucose in the feed resulted in ethanol production and reduced the biomass yield, whereas limiting glucose concentrations resulted in higher consumption of xylose and acetic acid. The specific growth rate, in the range of 0.013-0.015/h, did not appear to be influenced by the composition of the carbon source. Under anaerobic conditions, an ethanol yield of 0.40 g/g was obtained. The present strategy benefits from the easier separation of the biomass from the medium and the fungus ability to assimilate carbon residuals in comparison with when yeast is used. More specifically, it allows in-situ separation of insoluble solids leading to the production of pure fungal biomass as a value-added product. ARTICLE INFO ABSTRACT © 2016 BRTeam. All rights reserved.Journal homepage: www.biofueljournal.com FazeliNejad et al. / Biofuel Research Journal 9 (2016) [372][373][374][375][376][377][378] Please cite this article as: FazeliNejad S., Ferreira J.A., Brandberg T., Lennartsson P.R., Taherzadeh M.J. Fungal protein and ethanol from lignocelluloses using Rhizopus pellets under simultaneous saccharification, filtration and fermentation (SSFF).

Evaluation of Filamentous Fungal Biomass Cultivated on Vinasse as an Alternative Nutrient Source of Fish Feed: Protein, Lipid, and Mineral Composition

“…Most ethanol production occurred during the first 24 h, followed by its utilization by filamentous fungi as cultivant. Similar phenomena have been reported by FazeliNejad et al [43] for Rizhopus sp., utilizing ethanol as a carbon source after 48 h cultivation. From an industrial biorefinery viewpoint, ethanol produced through fungal cultivation in vinasse has the potential to be reintroduced into the molasses fermentation broth processed downstream, which has already been established to increase process efficiency.…”

Solid State Fermentation as a Tool to Stabilize and Improve Nutritive Value of Fruit and Vegetable Discards: Effect on Nutritional Composition, In Vitro Ruminal Fermentation and Organic Matter Digestibility

“…The current 15.7% increase in the CP content of the fermented substrate may be due to the high production of fungal cell mass, which led to a reduction of reducing sugars, and consequently the production of protein within the fungus population. This is in agreement with other previous studies [42][43][44] that demonstrated that some fungal species were able to increase the CP level in agro-industries wastes.…”

Lignocellulose integration to 1G-ethanol process using filamentous fungi: fermentation prospects of edible strain of Neurospora intermedia

“…Similar observations on the production of fungal biomass and ethanol using acid-pretreated wheat straw slurry were obtained in a previous study while using zygomycetes strains of filamentous fungus, Rhizopus sp. ; however the biomass yields obtained was only up to 0.34 g biomass/g consumed monomeric sugars and acetic acid [ 28 ].…”