Fungal pretreatments improve the efficiency of saccharification of <i>Panicum prionitis</i> Ness biomass

Gauna, Albertina; Larran, Alvaro Santiago; Perotti, Valeria; Feldman, Susana Raquel; Permingeat, Hugo R.

doi:10.1080/17597269.2018.1479934

Cited by 6 publications

(13 citation statements)

References 28 publications

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“…The purpose of the pretreatment is to remove lignin (Gauna et al, 2018;Nair and Sivakumar, 2020), thus when selecting organisms, attention was primarily focused on those isolates that were the best producers of ligninolytic enzymes. Among the 12 isolated fungi, the best ligninolytic activity (laccase, manganese peroxidase, and versatile peroxidase) showed two isolates, F13 and F28 (Figure 1 and 2), which were identified as Trametes hirsuta F13 and Stereum gausapatum F28, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Lignocellulosic waste as a renewable resource has attracted attention as a potential substrate for the production of valueadded chemicals, but its complex structure prevents its direct use; it needs to be pretreated to remove lignin and hydrolyzed before it could be used, which can be done by chemical, physical, physicochemical, or biological methods (Gauna et al, 2018;Nair and Sivakumar, 2020;Vučurović et al, 2019). The use of fungi or their enzymes in the pretreatment and hydrolysis of the lignocellulosic substrate is an ecologically safe method, and there is no formation of toxic components that can be hostile to the production microorganism (Nair and Sivakumar, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…So far, a sufficiently efficient fungal strain has not been found, nor the conditions that would enable the industrial application of this organism in the treatment of lignocellulosic biomass have been formulated. Fungi that belong to the genera Trametes, Ganoderma, Pleurotus have proved to be good candidates for lignocellulose pretreatment use (Gauna et al, 2018;Isroi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Pretreatment of lignocellulosic biomass with autochthonous fungi from Serbia

Jović¹,

Kocić‐Tanackov²,

Mojović³

2021

J Proc Ener Agri

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This research examined the potential use of isolated Serbian autochthonous fungi in lignocellulosic biomass pretreatment. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of a waste lignocellulosic biomass. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and it was selected as a potential producer of important industrial hydrolytic enzymes. Further, the breakdown of lignocellulosic waste, beechwood sawdust, by T. hirsuta F13 and S. gausapatum F28 was examined. Both isolates efficiently degraded biomass, but T. hirsuta F13 exhibited greater selectivity (selectivity coefficient of 1.7) than S. gausapatum F28 (1.1). The isolate F13 was considered a better candidate for the pretreatment, and it was selected for further analysis which involved the use of molasses stillage as a supplement to improve the pretreatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pretreatment of lignocellulosic biomass with autochthonous fungi from Serbia

Jović¹,

Kocić‐Tanackov²,

Mojović³

2021

J Proc Ener Agri

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“…Total glucose released with fungal secretomes obtained in IM. Numbers in brackets indicate the total glucose released with fungal secretomes obtained in PG medium (Gauna et al, 2018). Error bars represent the standard error.…”

Section: Resultsmentioning

confidence: 99%

“…The use of P. sanguineus secretome obtained in IM allowed a 22.5-fold and a 3-fold increase in glucose release compared to the secretome produced in PG medium over green and senescent biomass, respectively (Gauna et al, 2018) (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Secretome characterization of the lignocellulose-degrading fungi Pycnoporus sanguineus and Ganoderma applanatum

Gauna

Larran

Feldman

et al. 2020

Preprint

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C4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuels production. Although they display a high polysaccharide content and reach great levels of biomass accumulation, there is a major technical issue to be solved before they can be considered as biofuels feedstock: lignin removal. Concerning this, Pycnoporus and Ganoderma fungal genera have been highlighted due to their ability to hydrolyze lignocellulose. The goals here were to evaluate the pretreatment efficiency using P. sanguineus and G. applanatum secretomes harvested from a glucose-free inductive medium and to identify the fungal enzymatic activities responsible for the lignin degradation and glucose release. The findings show that P. sanguineus secretome exhibits a higher activity of lignocellulolytic enzymes compared to the one from G. applanatum. Interestingly, zymograms in presence of glucose suggest that a β-glucosidase isoform from P. sanguineus could be glucose-tolerant. The proteomic approach carried out allowed to identify 73 and 180 different proteins for G. applanatum and P. sanguineus secretomes, respectively, which were functionally classified in five main categories, and a miscellaneous group. Many uncharacterized proteins were found in both secretomes, reflecting that greater research is still needed for a better comprehension of lignocellulose degradation.

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