Fungal pathogens of Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae)

Abstract

European June beetle, Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae) is one of the most important soilborne pests in many parts of Turkey and the world. Entomopathogenic fungi are important microbial control agents that can be used to control soilborne pests, and it is desirable to obtain them from local insect populations. The study was conducted at Karadeniz Technical University, Faculty of Science, Microbiology Laboratory in 2017. In this study, fungal pathogens of A. solstitiale were investigated to find an effective microbial control agent. Fungi were isolated from infected larvae and morphological-molecular characterization of the isolates showed that all isolates were Metarhizium flavoviride Gams & Roszypal (Deuteromycotina: Hyphomycetes). Using phylogenetic analysis and pathogenicity tests, the isolates were found to be different genotypes of M. flavoviride. All isolates gave more than 80% mortality at a concentration of 10 6 conidia/ml, with one isolate (As2) causing 96% mortality. Therefore, dose-mortality experiments were conducted with As2, and the median lethal concentration was determined to be 3.87 x 10 3 conidia/ml. This study demonstrated that M. flavoviride As2 is an effective microbial control agent that can be used for biological control of A. solstitiale.

Biocontrol of the western conifer seed bug, Leptoglossus occidentalis Heidemann (Heteroptera: Coreidae) using indigenous entomopathogenic fungi

“…The white conidia were initially observed on the insect cadaver 6 days after treatment in the moist chamber, then green conidia were observed 10 days after treatment Eski et al Egyptian Journal of Biological Pest Control (2022) 32:140 bassiana (SUA a38) isolate was less virulent and LC 50 value was 8.46 × 10 6 conidia/ml. Generally, isolates obtained from naturally infected insects are more virulent than those isolated from different habitats (Biryol et al 2020). As in this study, EPF isolated from a particular host or soil have shown to be virulent to other hosts.…”

“…Biryol et al (2020) As18A. solstitialeBiryol et al (2020) Metarhizium brunneum Gg12 Gryllotalpa grylotalpa (Orthoptera: Gryllotalpidae)Eski et al Egyptian Journal of Biological Pest Control (2022) 32:140 …”

The UV protectant properties of tea extracts on entomopathogenic fungus spores and their lethal effect on Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)

“…In the current study, we examined the effectiveness of four tea extracts as UV protectants using B. bassiana Pa4 and M. flavoviride As-2 isolates and their mortality on G. mellonella larvae. These isolates have demonstrated significant potential in previous studies focusing on biological control (Biryol et al, 2020(Biryol et al, , 2021.…”

“…In previous studies, B. bassiana (Pa4) and M. flavoviride (As-2) were isolated from Pristiphora abietina (Christ, 1791) (Hymenoptera: Tenthredinidae) and Amphimallon solstitialis (Linnaeus, 1758) (Coleoptera: Scarabaeidae) in Türkiye, respectively. These fungi exhibited significant insecticidal activity on insect pests (P. abietina and A. solstitialis) (Biryol et al, 2020(Biryol et al, , 2021.…”

Stability ofMetarhizium anisopliae(Hypocreales: Clavicipitaceae) isolates during repeatedin vitrosubculture and evaluation of an oil-in-water mycoinsecticide

“…Control larvae were treated with 0.01% aqueous Tween 80. Assessments were conducted at 28 ± 2 °C and 16:8-hour light:dark photoperiod for 15 days (Biryol et al 2020). Dead insects were removed and placed in Petri dishes with moist filter paper to allow mycosis to occur.…”