Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity

Self Cite

Microbial mediated synthesis of metallic nanoparticles constitutes as effective and promising approach for the development of antibacterial materials in the field of bioengineering and biomedicine. We prepared Cadmium oxide nanoaprticles (CdO NPs) utilizing Penicillium oxalicum, and cadmium acetate solution via coprecipitate method. The elemental composition and morphology of these synthesized CdO NPs were examined through X-ray diffraction (XRD), UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Energy dispersive spectroscopy (EDS). Furthermore, we evaluated the bactericidal potential of prepared CdO NPs using Escherichia coli (E.coli), Staphylococcus aureus (S.aureus), Bacillus cereus (B.cereus), and Pseudomonas aeruginosa (P. aeruginosa). Dimethyl sulfoxide was used as negative control while erythromycin was used as positive control. The XRD spectrum revealed cubic crystalline nanoparticles with 22.94 nm size and UV showed absorbance peak at 297 nm with 2.5 eV band gap energy. FTIR depicted O─H and carboxylic groups along with CdO stretching vibration. EDS showed the presence of organic compounds on Cd and O over NPs surface. SEM results revealed the spherical shape of the CdO NPs. The synthesized NPs exhibited highly potent bactericidal activity against selected strains and demonstrated less optical density of 0.086 after 24 hr. Owing to the significant antibacterial activity of CdO NPs, the broad application prospects of these nanoparticles CdO NPs in extensive biomedical applications is indicated.

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of microbial mediated cadmium oxide nanoparticles

Asghar

Habib

Zaman

et al. 2020

Self Cite

“…Biofungicides also reduce the risk of developing the pathogen resistance (Iravani, 2011). With the advent of nanotechnology, scientists are working on the use of nanoparticles (NPs) to inhibit the growth of different pathogens (Feroze et al, 2020). NPs can be characterized by scanning electron microscopy and other techniques (Deng, Zhang, Jiang, Ma, & Mao, 2019).…”

mentioning

confidence: 99%

Scanning electron microscopy of bio‐fabricated Fe₂O₃ nanoparticles and their application to control brown rot of citrus

Ali

Haroon

Khizar

et al. 2020

Citrus is the leading fruit crop of Pakistan and exported to different parts of the world. Due to suitable weather condition, this crop is affected by different biotic factors which seriously deteriorate its quality and quantity. During the months of November 2018 to January 2019, citrus brown rot symptoms were recurrently observed on sweet oranges in National Agricultural Research Centre (NARC), Islamabad. Causal agent of citrus brown rot was isolated, characterized, and identified as Fusarium oxysporum. For environment-friendly control of this disease, leaf extract of Azadirachta indica was used for the green synthesis of iron oxide (Fe 2 O 3) nanoparticles. These nanoparticles were characterized before their application for disease control. Fourier transform infrared spectroscopy (FTIR) of these synthesized nanoparticles described the presence of stabilizing and reducing compounds like alcohol, phenol, carboxylic acid, and alkaline and aromatic compounds. X-Ray diffraction (XRD) analysis revealed the crystalline nature and size (24 nm) of these nanoparticles. Energy dispersive X-Ray (EDX) analysis elaborated the presence of major elements in the samples. Scanning electron microscopy (SEM) confirmed the spinal shaped morphology of prepared nanoparticles. Successfully synthesized nanoparticles were evaluated for their antifungal potential. Different concentrations of Fe 2 O 3 nanoparticles were used and maximum mycelial inhibition was observed at 1.0 mg/ml concentration. On the basis of these findings, it could be concluded that Fe 2 O 3 nanoparticles, synthesized in the leaf extract of A. indica, can be successfully used for the control of brown rot of sweet oranges.

“…Characterization of synthesized AgNPs was performed by using different material characterization techniques to explore their morphologies. SEM JEOL 7500F HRSEM was used to manifest the size and shape of silver nanoparticles, and the colloidal mixture was dropped on a copper grid, dried with filter paper and images were captured on different voltages and powers of the microscope (Feroze et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Phytosynthesis of colloidal nanosilver from Mentha longifolia and Mentha arvensis: Comparative morphological and optical characterization

Javed

Mashwani

2020

The present study involves an ecofriendly strategy for the reduction of bulk silver into its nanoforms by using the aqueous extracts of Mentha longifolia leaves (MLL), M. longifolia branches (MLB), and Mentha arvensis (MA). Synthesis of silver nanoparticles (AgNPs) was confirmed initially by observing a change in the color of the reaction mixture followed by measuring the absorbance, and a characteristic surface plasmon resonance band was observed between 400 and 500 nm of the λ of light. Morphological and optical characterization techniques of AgNPs were performed by using UV-visible spectrophotometer, scanning electron microscopy, atomic force microscopy, energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) analysis. It was confirmed that the phytosynthesized nanoparticles are anisotropic and nearly spherical having a size smaller than 100 nm while some cubical and prismatic nanostructures were also observed. The average size of a single nanoparticle measured by DLS analysis was reported 10.50 nm, 15.55 nm, and 20.46 nm biofabricated by using MLB, MLL, and MA extract, respectively. The EDX analysis reported the presence of elemental Ag while elemental O was also observed in MLL and MLB AgNPs. The results from these experiments endorse the potential of reported plant species to phytosynthesize AgNPs. The future applications of this work involve the utilization of AgNPs for multiple biological applications.