Fungal laccases

Rivera-Hoyos, Claudia M.; Morales-Álvarez, Edwin D.; Poutou-Piñales, Raúl A.; Pedroza-Rodríguez, Aura M.; Rodrı́guez-Vázquez, Refugio; Delgado-Boada, Julio M.

doi:10.1016/j.fbr.2013.07.001

Cited by 208 publications

(201 citation statements)

References 138 publications

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“…Laccase (p-diphenol: dioxygen oxidoreductase, EC 1.10.3.2) belongs to the family of copper-containing oxidases, which catalyze the 1-electron oxidation of a wide range of inorganic and organic substances, coupled with a 4-electron reduction of oxygen to water [1][2][3][4]. This enzyme is among the most studied redoxases and has been used successfully as a commercial industrial catalyst.…”

Section: Introductionmentioning

confidence: 99%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe 3 O 4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe 3 O 4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe 3 O 4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 • C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe 3 O 4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.

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Section: Introductionmentioning

confidence: 99%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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“…The reactions catalyzed by laccase and its broad range of substrate specificity have made it an indispensable tool in environmental protection processes, chemical, nanobiotecnology and manufacturing industries [15]. Such applications include the removal of lignin and bleaching in paper production, removal of highly recalcitrant environmental pollutant and xenobiotics, dye decolorization, water purification system, production of household materials, production of anticancer drugs, biopolymer modification, wine classification, and the production of nanoparticles and biosensors [13,15,16].…”

Section: Introductionmentioning

confidence: 99%

“…It is a glycoprotein, which is ubiquitous in fungi, plants, insect [12,13] and has been reported in a few bacteria [14]. The reactions catalyzed by laccase and its broad range of substrate specificity have made it an indispensable tool in environmental protection processes, chemical, nanobiotecnology and manufacturing industries [15].…”

Section: Introductionmentioning

confidence: 99%

Chitosan multiple addition enhances laccase production from Trametes versicolor

Adekunle

Wang

et al. 2015

Bioprocess Biosyst Eng

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Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L -1 chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l -1 was achieved on the seventh day and laccase production was improved by 93.5 % higher than the control. Chitosan treatment increased reactive oxygen species generation and extracellular protein concentration in the treated mycelia. In contrast, the inducer inhibited the mycelia growth. The result of the quantitative reverse transcription polymerase chain reaction showed that the copy number of the laccase gene transcript increased by 16.7-fold in the treated mycelia relative to the control. This study provides insight into some of the intrinsic metabolic processes involved in the upregulation of laccase production in the presence of chitosan inducer in fungal culture.

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“…Laccase is a multicopper enzyme commonly found in plants, fungi, and bacteria [1]; this enzyme is related to diverse functions, such as lignin synthesis and plant protection structures, sporulation, lignin degradation, and pathogenesis (fungi), of the mentioned organisms [2,3]. e laccases have been studied for possible applications in the paper industry, due to their lignin degradation capacity [4], as well as in the treatment of wastewater contaminated by phenolic compounds [5].…”

Section: Introductionmentioning

confidence: 99%

Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

Juárez-Gómez

Rosas-Tate

Roa‐Morales

et al. 2018

Journal of Chemistry

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e noncompetitive inhibition of laccase by mercury ions is reported, in particular focusing their effect over the enzyme catalytic activity. e enzymatic kinetics were obtained for different substrates (caffeic acid, gallic acid, and catechol), where caffeic acid displayed the greatest enzymatic activity. e laccase inhibition by mercury ions permitted to establish the inhibition effect through a mixed model (that actually displayed a behavior closer to that of the noncompetitive inhibitors) when evaluated by means of UV-Vis spectrophotometry, using caffeic acid as an electron donor. A mercury concentration of 2 mM led to 35% enzymatic inhibition after only a 2-minute incubation period. is method was used for quantification of mercury ions in aqueous solution, showing a detection limit of 15 ± 1 ppm. erefore, this work presented a novel perspective for the determination of the toxic Hg(II) ions that can be readily implemented into environmental remediation methods.

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Fungal laccases

Cited by 208 publications

References 138 publications

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Chitosan multiple addition enhances laccase production from Trametes versicolor

Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

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