Fungal laccase: properties and activity on lignin

Leonowicz, Andrzej; Cho, NamSeok; Luterek, Jolanta; Wilkolazka, A.; Wojtas-Wasilewska, M.; Matuszewska, Anna; Hofrichter, Martin; Wesenberg, Dirk; Rogalski, Jerzy

doi:10.1002/1521-4028(200107)41:3/4<185::aid-jobm185>3.0.co;2-t

Cited by 483 publications

(260 citation statements)

References 192 publications

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“…The VP acts on manganese but also oxidizes a variety of phenolics and non-phenolics independently along with the oxidation of substituted phenols. The last enzyme to be discussed here is Laccase (EC 1.10.3.2), a copper containing enzyme that performs one electron oxidization of phenolic and non-phenolic substrates, while reducing O 2 to H 2 O [188]. Laccase also has demethylating and demethoxylating activities [188].…”

Section: +mentioning

confidence: 99%

“…The last enzyme to be discussed here is Laccase (EC 1.10.3.2), a copper containing enzyme that performs one electron oxidization of phenolic and non-phenolic substrates, while reducing O 2 to H 2 O [188]. Laccase also has demethylating and demethoxylating activities [188]. Lignin degrading fungi produce laccase under ligninolytic conditions hinting at the possibility that laccases play a role in lignin degradation [188].…”

Section: +mentioning

confidence: 99%

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Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

et al. 2010

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Phenylpropanoid metabolism yields a mixture of monolignols that undergo chaotic, non-enzymatic reactions such as free radical polymerization and spontaneous selfassembly in order to form the polyphenolic lignin which is a barrier to cost-effective lignocellulosic biofuels. Post-synthesis lignin integration into the plant cell wall is unclear, including how the hydrophobic lignin incorporates into the wall in an initially hydrophilic milieu. Self-assembly, self-organization and aggregation give rise to a complex, 3D network of lignin that displays randomly branched topology and fractal properties. Attempts at isolating lignin, analogous to archaeology, are instantly destructive and nonrepresentative of in planta. Lack of plant ligninases or enzymes that hydrolyze specific bonds in lignin-carbohydrate complexes (LCCs) also frustrate a better grasp of lignin. Supramolecular self-assembly, nano-mechanical properties of lignin-lignin, ligninpolysaccharide interactions and association-dissociation kinetics affect biomass deconstruction and thereby cost-effective biofuels production. OPEN ACCESSMolecules 2010, 15 8642

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Section: +mentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

et al. 2010

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“…Further, our conclusions are based on relative increases in organic substrate utilization potential in response to treatments that reduce host photosynthetic capacity. These conclusions are not diminished if measures represent increases in broadly deWned "peroxidases", a class of enzymes that work in cooperation with laccase in the complex process of wood deligniWcation (reviewed by Leonowicz et al 2001). Rather, as Dr Baldrian rightly acknowledges, they indicate signiWcant increases in the potential for EM fungi to exploit cellulose, protein and phenolic substrates in response to treatments in Weld settings.…”

Section: Ecological Relevance Of Secreted Enzymes For Em Fungimentioning

confidence: 55%

Saprotrophic capabilities as functional traits to study functional diversity and resilience of ectomycorrhizal community

Cullings

Courty

2009

Oecologia

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In an accompanying editorial Dr Petr Baldrian made a case casting doubt on our recent work addressing the saprophytic potential of ectomycorrhizal (EM) fungi. Dr Baldrian's statements illustrate a very valid truth: the book is still very much open on this subject. The point he raised that the only logical reason for these fungi to be responding to high carbon demand or decreased host photosynthetic capacity by up-regulating enzymes is for the purpose of carbon acquisition is valid as well. Despite this, he makes the case that there is no compelling evidence that EM fungi exhibit saprophytic activity. The concept central to Dr Baldrian's conclusion is that even though some EM fungi possess the genes necessary for saprophytic behaviour and may even express these genes, EM fungi do not inhabit a position in the soil column that provides access to usable substrate. In this paper we present both previously published and newly obtained data that demonstrate that this assumption is erroneous, and we present arguments that place the saprophytic potential of EM fungi within a broad ecological context.

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“…The data obtained in this work showed that laccase was the main ligninolytic enzyme produced by P. pulmonarius in submerged cultures, considering that low Mn peroxidase activities and no lignin peroxidase were detected in the culture filtrates. In the genus Pleurotus, laccases are constitutively produced in small amounts; however, their production can be considerably enhanced by a wide variety of substances, mainly aromatic or phenolic compounds related to lignin or lignin derivatives, such as ferulic acid, 2,5 xylidine, p-anisidine or veratryl alcohol (Leonowicz et al, 2001;Souza et al, 2004;Tychanowicz et al, 2006) In this work, the supplementation of submerged cultures with corn cob extract had a very pronounced effect on the laccase activity in P. pulmonarius. The laccase inducer effect of the soluble plant extract is considered to be related to its phenolic content.…”

Section: Discussionmentioning

confidence: 99%

Pentachlorophenol removal by Pleurotus pulmonarius in submerged cultures

Souza

Costa

Madrona

et al. 2011

Braz. arch. biol. technol.

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Pentachlorophenol (PCP) removal by Pleurotus pulmonarius grown in submerged cultures in the presence and absence of laccase inducers was studied in this work. When PCP was added to a final concentration of 25 mg·L -1 in submerged cultures actively producing laccase, 70% of the PCP was removed after 96 h. The removal of PCP was less than 20% in the cultures with low laccase activity. The results suggested that laccase played an important role in the biodegradation of PCP by P. pulmonarius and that for bioremediation purposes the fungus must be cultured under the conditions of active laccase production.

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Fungal laccase: properties and activity on lignin

Cited by 483 publications

References 192 publications

Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

Saprotrophic capabilities as functional traits to study functional diversity and resilience of ectomycorrhizal community

Pentachlorophenol removal by Pleurotus pulmonarius in submerged cultures

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