Fungal isolation and causal organisms of zearalenone contamination from Job's tears seeds and the products

Narita, N; Suzuki, Hodaka; Kikuchi, Yutaka; Ichinoe, Masakatsu; Ikebuchi, Hideharu; Tanaka, T.; Sawada, Jun‐ichi

doi:10.2520/myco1975.1992.36_39

Cited by 3 publications

(2 citation statements)

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“…It has been well documented that Job's-tears products can be contaminated by mycotoxins (Tanaka et al, 1985a;Narita et al, 1992). It has been demonstrated that the present ELISA for zearalenone was sensitive and facile because no cleanup procedure (method B) of barley and Job's-tears samples was used and because a purified solid phase antigen was used.…”

Section: Discussionmentioning

confidence: 85%

Sensitive Enzyme-Linked Immunosorbent Assay for the Mycotoxin Zearalenone in Barley and Job's-Tears

Tanaka¹,

Teshima²,

Ikebuchi³

et al. 1995

J. Agric. Food Chem.

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An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for zearalenone in barley and Job's-tears was established. To improve sensitivity of the assay system for zearalenone, a solid phase antigen was prepared by affinity purification. This assay system uses a purified antigen and specific monoclonal antibodies for zearalenone. Consequently, the detection limit of zearalenone by CI-ELISA was increased to 0.2 ng/mL (equivalent to 10 ng/g in barley and Job's-tears). Zearalenone in barley and Job's-tears samples could be determined in the range of 25-1000 ng/g using method A (methanol-water and dichloromethane extraction) and method B (methanol-water alone) as sample preparation. The average recoveries of zearalenone from barley samples were observed to be 106% (mean CV, 10.3%) by method A and 98% (mean CV, 7.5%) by method B; also, those of zearalenone from Job's-tears samples were observed to be 96% (mean CV, 9.4%) by method A and 102% (mean CV, 6.5%) by method B. There was little or no difference between method A and method B for the recovery. For the benefit of a facile sample preparation, unknown amounts of zearalenone in barley and Job's-tears samples were assayed by method B. The results obtained by CI-ELISA were closely correlated with those of high-performance liquid chromatography.

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Section: Discussionmentioning

confidence: 85%

Sensitive Enzyme-Linked Immunosorbent Assay for the Mycotoxin Zearalenone in Barley and Job's-Tears

Tanaka¹,

Teshima²,

Ikebuchi³

et al. 1995

J. Agric. Food Chem.

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“…Since there was no correlation between the colony number of F. oxysporum and zearalenone level (Table 2), F. oxysporum was not examined. In contrast, F. verticillioides was examined because it has been reported to have zearalenone-producing ability 11) . Our cultivation study of the isolated F. semitectum and F. verticillioides (Table 3) indicated that F. semitectum, which is frequently detected in plant tissues from warmer regions 12) , caused zearalenone contamination of sorghum imported into Japan.…”

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confidence: 99%

Zearalenone Contamination and the Causative Fungi in Sorghum

Aoyama¹,

Ishikuro²,

Nishiwaki³

et al. 2009

Journal of the Food Hygienics Society of Japan

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Natural contamination by zearalenone, a toxic metabolite of Fusarium fungi, was surveyed in 160 samples of sorghum imported from 2001 to 2006 into Japan for feed. Of these 160 samples, 84 (52.5῍) were contaminated with zearalenone, ranging in concentration from 60 to 7,260 mg/kg. In the contaminated sorghum samples, F. semitectum, F. verticillioides, F. oxysporum, and other Fusarium spp. were detected. The concentration of zearalenone was well correlated with the development of colonies of F. semitectum and other Fusarium spp. When the isolates of F. semitectum and F. verticillioides were cultivated on sorghum, zearalenone was found only in F. semitectum culture. These results indicate that F. semitectum is a causal fungus of zearalenone contamination in sorghum.

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Proposal for Studies of Mycotoxigenic Fungi in Foods

Ichinoe¹

2010

Journal of the Food Hygienics Society of Japan

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Fungal isolation and causal organisms of zearalenone contamination from Job's tears seeds and the products

Cited by 3 publications

References 0 publications

Sensitive Enzyme-Linked Immunosorbent Assay for the Mycotoxin Zearalenone in Barley and Job's-Tears

Sensitive Enzyme-Linked Immunosorbent Assay for the Mycotoxin Zearalenone in Barley and Job's-Tears

Zearalenone Contamination and the Causative Fungi in Sorghum

Proposal for Studies of Mycotoxigenic Fungi in Foods

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