Fungal-induced protein hyperacetylation in maize identified by acetylome profiling

Walley, Justin W.; Shen, Zhouxin; McReynolds, Maxwell R; Schmelz, Eric A.; Briggs, Steven P.

doi:10.1073/pnas.1717519115

Cited by 78 publications

(71 citation statements)

References 88 publications

(153 reference statements)

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“…S7B). Our finding that REL2 may transcriptionally regulate many defense-related gene agrees with a recent study implicating REL2 in the response to the fungal pathogen Cochliobolus carbonum (Walley et al, 2018).…”

Section: Key Tfs Are Misregulated In Rel2 Tasselssupporting

confidence: 92%

RAMOSA1 ENHANCER LOCUS2-Mediated Transcriptional Repression Regulates Vegetative and Reproductive Architecture

et al. 2018

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Transcriptional repression in multicellular organisms orchestrates dynamic and precise gene expression changes that enable complex developmental patterns. Here, we present phenotypic and molecular characterization of the maize (Zea mays) transcriptional corepressor RAMOSA1 ENHANCER LOCUS2 (REL2), a unique member of the highly conserved TOPLESS (TPL) family. Analysis of single recessive mutations in rel2 revealed an array of vegetative and reproductive phenotypes, many related to defects in meristem initiation and maintenance. To better understand how REL2-mediated transcriptional complexes relate to rel2 phenotypes, we performed protein interaction assays and transcriptional profiling of mutant inflorescences, leading to the identification of different maize transcription factors and regulatory pathways that employ REL2 repression to control traits directly impacting maize yields. In addition, we used our REL2 interaction data to catalog conserved repression motifs present on REL2 interactors and showed that two of these, RLFGV-and DLN-type motifs, interact with the C-terminal WD40 domain of REL2 rather than the N terminus, which is known to bind LxLxL EAR motifs. These findings establish that the WD40 domain of TPL family proteins is an independent protein interaction surface that may work together with the N-terminal domain to allow the formation of large macromolecular complexes of functionally related transcription factors.

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Section: Key Tfs Are Misregulated In Rel2 Tasselssupporting

confidence: 92%

RAMOSA1 ENHANCER LOCUS2-Mediated Transcriptional Repression Regulates Vegetative and Reproductive Architecture

et al. 2018

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“…It is well known that plant immune response during pathogen infection requires extensive transcriptional reprogramming involving histone acetylation. Pathogens interfere with this process by using effector proteins encoding acetyltransferases that can directly acetylate host proteins to alter immunity [ 90 , 91 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome dynamics associated with resistance and susceptibility against fusarium head blight in four wheat genotypes

Pan¹,

Liu²,

Rocheleau

et al. 2018

BMC Genomics

120

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BackgroundFusarium head blight (FHB) of wheat in North America is caused mostly by the fungal pathogen Fusarium graminearum (Fg). Upon exposure to Fg, wheat initiates a series of cellular responses involving massive transcriptional reprogramming. In this study, we analyzed transcriptomics data of four wheat genotypes (Nyubai, Wuhan 1, HC374, and Shaw), at 2 and 4 days post inoculation (dpi) with Fg, using RNA-seq technology.ResultsA total of 37,772 differentially expressed genes (DEGs) were identified, 28,961 from wheat and 8811 from the pathogen. The susceptible genotype Shaw exhibited the highest number of host and pathogen DEGs, including 2270 DEGs associating with FHB susceptibility. Protein serine/threonine kinases and LRR-RK were associated with susceptibility at 2 dpi, while several ethylene-responsive, WRKY, Myb, bZIP and NAC-domain containing transcription factors were associated with susceptibility at 4 dpi. In the three resistant genotypes, 220 DEGs were associated with resistance. Glutathione S-transferase (GST), membrane proteins and distinct LRR-RKs were associated with FHB resistance across the three genotypes. Genes with unique, high up-regulation by Fg in Wuhan 1 were mostly transiently expressed at 2 dpi, while many defense-associated genes were up-regulated at both 2 and 4 dpi in Nyubai; the majority of unique genes up-regulated in HC374 were detected at 4 dpi only. In the pathogen, most genes showed increased expression between 2 and 4 dpi in all genotypes, with stronger levels in the susceptible host; however two pectate lyases and a hydrolase were expressed higher at 2 dpi, and acetyltransferase activity was highly enriched at 4 dpi.ConclusionsThere was an early up-regulation of LRR-RKs, different between susceptible and resistant genotypes; subsequently, distinct sets of genes associated with defense response were up-regulated. Differences in expression profiles among the resistant genotypes indicate genotype-specific defense mechanisms. This study also shows a greater resemblance in transcriptomics of HC374 to Nyubai, consistent with their sharing of two FHB resistance QTLs on 3BS and 5AS, compared to Wuhan 1 which carries one QTL on 2DL in common with HC374.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5012-3) contains supplementary material, which is available to authorized users.

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“…In this study, we characterized the auxin‐regulated proteome in Arabidopsis by treating 5‐day‐old seedlings grown on 0.5× Murashige and Skoog (MS) media overlaid with nylon mesh with 1 µ m indole‐3‐acetic acid (IAA), a naturally occurring auxin (“auxin treated”), or an equivalent volume of 95% ethanol diluted in 0.5× MS (“mock” treated) for 3 h ( Figure A). Proteins were extracted and processed into peptides, which were iTRAQ 4‐plex labeled, pooled, and then identified and quantified using tandem mass spectrometry (MS/MS) (Figure A) according to previously utilized methods . These datasets have been deposited at MassIVE (MassIVE ID: MSV000079857).…”

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confidence: 99%

Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

et al. 2019

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It is known that auxin induces rapid gene expression changes throughout plant development, but how these transcriptional responses relate to changes in protein abundance is not well characterized. This report identifies early auxin responsive proteins in whole Arabidopsis seedlings using an isobaric tags for relative and absolute quantification‐based quantitative proteomics approach. Approximately 25% of the detected proteins (1045 out of 4257 proteins) are auxin responsive, which is in line with the central role of auxin in the regulation of plant growth and development. Several well‐known auxin pathway proteins are identified as differentially expressed, validating this quantitative proteomics approach. Additionally, functional categorization of these auxin responsive proteins indicates that rapid and complex metabolic changes occur in seedlings in response to auxin, including lipid metabolism. Altogether, these data describe novel auxin‐regulated proteins and are an excellent resource for identifying new downstream signaling components related to auxin‐mediated plant growth and development.

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Fungal-induced protein hyperacetylation in maize identified by acetylome profiling

Cited by 78 publications

References 88 publications

RAMOSA1 ENHANCER LOCUS2-Mediated Transcriptional Repression Regulates Vegetative and Reproductive Architecture

RAMOSA1 ENHANCER LOCUS2-Mediated Transcriptional Repression Regulates Vegetative and Reproductive Architecture

Transcriptome dynamics associated with resistance and susceptibility against fusarium head blight in four wheat genotypes

Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

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