Fungal Histidine Kinases

Santos, Jennifer L.; Shiozaki, Kazuhiro

doi:10.1126/scisignal.982001re1

Cited by 22 publications

(24 citation statements)

References 129 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eukaryotes have been thought to rely mainly on serine, threonine, and tyrosine kinases for signal transduction, but histidine kinase two-component systems have recently been shown to play a role in environmental sensing and cell development in eukaryotes (26), for example, in Candida albicans, where they regulate filamentation (18,19). We show that a histidine kinase regulates sensing of environmental changes needed for mold-to-yeast transition in at least two dimorphic fungal pathogens.…”

Section: And E)mentioning

confidence: 99%

Global Control of Dimorphism and Virulence in Fungi

Nemecek

Wüthrich

Klein

2006

Science

331

329

View full text Add to dashboard Cite

Microbial pathogens that normally inhabit our environment can adapt to thrive inside mammalian hosts. There are six dimorphic fungi that cause disease worldwide, which switch from nonpathogenic molds in soil to pathogenic yeast after spores are inhaled and exposed to elevated temperature. Mechanisms that regulate this switch remain obscure. We show that a hybrid histidine kinase senses host signals and triggers the transition from mold to yeast. The kinase also regulates cell-wall integrity, sporulation, and expression of virulence genes in vivo. This global regulator shapes how dimorphic fungal pathogens adapt to the mammalian host, which has broad implications for treating and preventing systemic fungal disease.

show abstract

Section: And E)mentioning

confidence: 99%

Global Control of Dimorphism and Virulence in Fungi

Nemecek

Wüthrich

Klein

2006

Science

331

329

View full text Add to dashboard Cite

show abstract

“…This system is highly related to two-component signaling pathways prevalent in prokaryotes. However, similar to Saccharomyces cerevisiae (41), a more complex phosphorelay is present in S. pombe (44), which comprises of three hybrid histidine kinases: two highly related proteins Mak2 and Mak3, and a third kinase Mak1 (1,8); the phosphorelay protein Mpr1, and two response regulators Mcs4 (38) and Prr1. Based on studies of the Sln1 phosphorelay system in S. cerevisiae (41), stimulation of the sensor kinase is predicted to result in autophosphorylation of a conserved histidine residue, which is subsequently transferred to an aspartic acid residue in the receiver domain of the histidine kinase.…”

Section: Introductionmentioning

confidence: 98%

“…Many of the signaling pathways that regulate transcriptional responses to H 2 O 2 in S. pombe are conserved in higher eukaryotes and include the mammalian p38=JNK-related Sty1 (also known as Phh1 and Spc1) stress-activated protein kinase (SAPK) pathway (34,48), which phosphorylates and regulates the bZip transcription factor Atf1 (49,53), and the AP-1-like transcription factor Pap1 (55,56), which is related to mammalian Jun. S. pombe also contains a multi-step two-component related system (1,8,38,44), comprised of three histidine kinases, Mak1 (Phk3), Mak2 (Phk1), and Mak3 (Phk2), a single phosphorelay protein Mpr1 (Spy1), and the response regulators Mcs4 and Prr1. Mak2, Mak3, Mpr1, and Mcs4 constitute a phosphorelay system that transduces H 2 O 2 signals to the Sty1 SAPK (8,38), whereas the response regulator transcription factor Prr1 directly regulates oxidative stress response genes independently of Sty1 (39,40).…”

Section: Introductionmentioning

confidence: 99%

Two-Component Mediated Peroxide Sensing and Signal Transduction in Fission Yeast

Quinn

Malakasi

Smith

et al. 2011

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

Two-component related proteins play a major role in regulating the oxidative stress response in the fission yeast, Schizosaccharomyces pombe. For example, the peroxide-sensing Mak2 and Mak3 histidine kinases regulate H(2)O(2)-induced activation of the Sty1 stress-activated protein kinase pathway, and the Skn7-related response regulator transcription factor, Prr1, is essential for activation of the core oxidative stress response genes. Here, we investigate the mechanism by which the S. pombe two-component system senses H(2)O(2), and the potential role of two-component signaling in the regulation of Prr1. Significantly, we demonstrate that PAS and GAF domains present in the Mak2 histidine kinase are essential for redox-sensing and activation of Sty1. In addition, we find that Prr1 is required for the transcriptional response to a wide range of H(2)O(2) concentrations and, furthermore, that two-component regulation of Prr1 is specifically required for the response of cells to high levels of H(2)O(2). Significantly, this provides the first demonstration that the conserved two-component phosphorylation site on Skn7-related proteins influences resistance to oxidative stress and oxidative stress-induced gene expression. Collectively, these data provide new insights into the two-component mediated sensing and signaling mechanisms underlying the response of S. pombe to oxidative stress.

show abstract

“…Membrane based and cytoplasmic (Bahn 2008;Catlett et al 2003) Protists Yes Characterized only in amoeba. Four HKs related to development (Santos and Shiozaki 2001;Thomason and Kay 2000) Microalgae Yes At least one in Chlamydomonas reinhardtii (GenBank XM_001701571) and two in the diatom Phaeodactylum tricornutum (GenBank XP_002185972 and XP_002179622)…”

Section: Yeast and Fungimentioning

confidence: 99%

“…Since there are excellent reviews for yeast osmosensing and regulation (Saito 2001;Santos and Shiozaki 2001;O'Rourke et al 2002;Gacto et al 2003;Westfall et al 2004;Hohmann et al 2007;Bahn 2008;Plemenitas et al 2014) we will cover this group for comparison.…”

Section: Introductionmentioning

confidence: 99%

Osmosensing and osmoregulation in unicellular eukaryotes

Suescún-Bolívar

Thomé

2015

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Eukaryotic microorganisms possess mechanisms to detect osmotic variations in their surroundings, from specialized receptors and membrane transporters, to sophisticated systems such as two-component histidine kinases. Osmotic stimuli are transduced through conserved phosphorylation cascades that result in a rapid response to mitigate stress. This response allows for the maintenance of an optimal biochemical environment for cell functioning, as well as a suitable recovery in suboptimal environments that would otherwise endanger cell survival. The molecular basis of these responses has been largely studied in yeasts and bacteria. However, fewer studies have been published concerning the molecular basis of osmoregulation in other eukaryotic microorganisms such as protozoans and microalgae. Here, we review the main osmosensors reported in unicellular eukaryotic microorganisms (yeasts, microalgae and protozoa) and the pathways that maintain homeostasis in cells encountering hyperosmotic challenges.

show abstract

Fungal Histidine Kinases

Cited by 22 publications

References 129 publications

Global Control of Dimorphism and Virulence in Fungi

Global Control of Dimorphism and Virulence in Fungi

Two-Component Mediated Peroxide Sensing and Signal Transduction in Fission Yeast

Osmosensing and osmoregulation in unicellular eukaryotes

Contact Info

Product

Resources

About