Abstract:Background and Aim: Post-harvest fungal infection of fruits and vegetables is mainly caused by fungal pathogens that can be harmful to both human and animals as they produce mycotoxins, post-harvest diseases in fruits and vegetables are a serious problem that results in the loss of a large percentage of crops reaching 50% in some fruits. This study aims at screening the post-purchasing shelf-life of four highly consumed fruits and vegetables and at identifying the fungal strains behind their spoilage in Qatar.… Show more
“…The origin of contamination could be via air, as both of the fresh bars were located inside shopping centers as open-space shops. It needs to be noted that air can play an important role in the transfer of spoilage microorganisms [ 52 , 53 ]. The second collected fruit smoothie from Fresh Bar F has also a higher occurrence of yeasts and fungi (FF1—strawberries, banana, orange) ( Table 3 ).…”
Among the many consumers in Slovakia, smoothies are nowadays gaining popularity. Smoothie drinks are prepared from raw fruits and vegetables. Therefore, their microbiological safety depends on hygiene standards. The aim of this work was to monitor and quantify selected sensitive and antibiotic-resistant microorganisms present in collected smoothies. Twenty analyzed smoothie samples were collected from six food service establishments (fresh bars) in the capital city of Slovakia, Bratislava. Antibiotic-resistant bacteria were found in at least one of each fresh bar. Antibiotic-resistant coliform bacteria prevailed, especially in green smoothies or juices containing more vegetable ingredients. Resistance to ampicillin, ciprofloxacin, tetracycline, chloramphenicol, and gentamicin was observed in the case of coliform bacteria. More than half of the smoothie drink samples did not contain resistant enterococci. On the other hand, vancomycin-resistant enterococci were detected in 20% of samples. The most frequently isolated antibiotic-resistant strains belonged to the Enterobacter spp. or Klebsiella spp. genus. In the last part of the work, the pretreatment effect of smoothie components on the selected microorganisms’ counts in the final product was investigated. Washing ingredients with an aqueous solution of a biocide agent containing silver and hydrogen peroxide proved to be the most effective way to decrease bacterial counts.
“…The origin of contamination could be via air, as both of the fresh bars were located inside shopping centers as open-space shops. It needs to be noted that air can play an important role in the transfer of spoilage microorganisms [ 52 , 53 ]. The second collected fruit smoothie from Fresh Bar F has also a higher occurrence of yeasts and fungi (FF1—strawberries, banana, orange) ( Table 3 ).…”
Among the many consumers in Slovakia, smoothies are nowadays gaining popularity. Smoothie drinks are prepared from raw fruits and vegetables. Therefore, their microbiological safety depends on hygiene standards. The aim of this work was to monitor and quantify selected sensitive and antibiotic-resistant microorganisms present in collected smoothies. Twenty analyzed smoothie samples were collected from six food service establishments (fresh bars) in the capital city of Slovakia, Bratislava. Antibiotic-resistant bacteria were found in at least one of each fresh bar. Antibiotic-resistant coliform bacteria prevailed, especially in green smoothies or juices containing more vegetable ingredients. Resistance to ampicillin, ciprofloxacin, tetracycline, chloramphenicol, and gentamicin was observed in the case of coliform bacteria. More than half of the smoothie drink samples did not contain resistant enterococci. On the other hand, vancomycin-resistant enterococci were detected in 20% of samples. The most frequently isolated antibiotic-resistant strains belonged to the Enterobacter spp. or Klebsiella spp. genus. In the last part of the work, the pretreatment effect of smoothie components on the selected microorganisms’ counts in the final product was investigated. Washing ingredients with an aqueous solution of a biocide agent containing silver and hydrogen peroxide proved to be the most effective way to decrease bacterial counts.
“…Bacterial and yeast strains taken from the laboratory collection previously isolated and identified or purchased, the collection is currently stored at Qatar University, Department of biological and environmental sciences, chosen microbial types include: E. coli ATCC 11775, Bacillus , S. aureus ATCC BAA976, P. microbilis and C. albicans . The fungal stains used were isolated and microscopically identified in a previous study (Table 6 ) 42 .…”
Fresh produces spoilage is a worldwide concern that accompany the global increase in food demand. Adverse human health and environmental effects of commercial spoilage control agents are major public concern. In this study, Prosopis juliflora leaves and fruit extracts had their antimicrobial activities evaluated against the growth of selected bacteria and yeast, and against mycelial growth and conidial germination of selected mycotoxins-producing fungi. P. juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with its novel extraction method showed the strongest antibacterial activity. Antimicrobial tests showed total inhibition of Botrytis cinerea, Alternaria alternata, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MICs ranging between 0.125 and 1 mg/ml. Percent inhibition of mycelial growth (PIMG) of the extract was also determined against seven other fungal strains with highest value against Geotrichum candidum (66.2%). Even the least affected fungal strain showed alterations in their hyphae and spores exposed to PJ-WS-LE extract when observed using scanning electron microscope (SEM), alterations include exfoliated flakes, pores, vacuolation and applanation. Small-scale fruit bioassays controlled experiment showed high efficacy of the extract in protecting inoculated cherry tomato samples from B. cinerea and A. alternata infections. In conclusion, PJ-WS-LE extract is a feasible, natural antifungal agent that can replace common anti-spoiling chemicals.
“…In addition, postharvest diseases of fruit and vegetables also pose a threat to human and animal health. Some pathogenic fungi, such as Aspergillus , Penicillium , Alternaria , and Fusarium , produce mycotoxins, like aflatoxin, ochratoxin A, patulin, Alternaria toxins, and fumonisin, which are nephrotoxic, genotoxic, teratogenic, carcinogenic, and cytotoxic 6–8 …”
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