Fungal Enzymes: Degradation and Detoxification of Organic and Inorganic Pollutants

Kumari, Rekha; Singh, Archana; Yadav, Ajar Nath

doi:10.1007/978-3-030-68260-6_5

Cited by 12 publications

(8 citation statements)

References 117 publications

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“…(2) Each system was annealed from 0 to 300 K for 50 ps with the NVT ensemble. (3) These systems were subjected to equilibration of 1 ns in an NPT ensemble at a target temperature of 300 K and a target pressure of 1 bar. The target temperature and target pressure were maintained with the Langevin thermostat [59] and the Berendsen barostat [60] .…”

Section: Methodsmentioning

confidence: 99%

Unravelling an Efficient O2/Reductant-Dependent Catalytic Route in Heme Peroxygenases for Sustainable Applications

Deng,

Jiang,

Kang

et al. 2024

Preprint

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Heme peroxygenases are attractive biocatalysts for incorporating oxygen into the organic molecules using H2O2 as oxygen source under mild conditions. However, their practical applications are hindered by irreversible oxidative inactivation caused by exogenous H2O2 usage. Herein, we report a novel catalytic pathway in heme peroxygenases that relies on O2 and small-molecule reductants such as ascorbate acid (AscA), dehydroascorbic acid (DHA), gallic acid (GA), or pyrogallol (PA) to drive reactions. For reactions of unspecific peroxygenase (UPO) with either AscA or DHA, experimental and computational studies revealed that DHAA (the hydrated form of DHA) is the actual co-substrate responsible for activating oxygen to generate oxyferryl heme (compound I, Cpd I) as the oxygenation species. Subsequently, we demonstrate the universality of this O2/reductant-dependent route across various heme peroxygenases, highlighting its biological significance as monooxygenases. Compared to the conventional H2O2-dependent process, this innovative route can efficiently eliminate the excessive production of H2O2, thereby preventing the heme destruction and related enzyme inactivation. Finally, scale-up reactions were performed for the preparations of chiral, value-added products with unprecedented productivity, underscoring the great synthetic capabilities of the developed peroxygenase technology, which paves the way for sustainable and practical applications in various chemical transformations.

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Section: Methodsmentioning

confidence: 99%

Unravelling an Efficient O2/Reductant-Dependent Catalytic Route in Heme Peroxygenases for Sustainable Applications

Deng,

Jiang,

Kang

et al. 2024

Preprint

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“…Fungi are easy to grow and produce an adequate amount of biomass for DDT remediation [32] . Certain fungal strains have shown the ability to degrade DDT [33] . Fungi are a great option for DDT-cleanup of contaminated soil and water because of two crucial characteristics.…”

Section: Introductionmentioning

confidence: 99%

The role and mechanisms of microbes in dichlorodiphenyltrichloroethane (DDT) and its residues bioremediation

Ebsa,

Gizaw,

Admassie

et al. 2024

Biotechnology Reports

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“…Basidiomycota and Ascomycota are the most prevalent fungal phyla involved in wood decay and thus have been studied mainly for bioremediation capacities [9]. Currently, the most commonly studied strains of wooddecaying basidiomycetes fungi for bioremediation are members of the genera Phanerochaete, Pleurotus, Trametes, and Gloeophyllum [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Genomic Diversity and Phenotypic Variation in Fungal Decomposers Involved in Bioremediation of Persistent Organic Pollutants

Lai

Neal

et al. 2023

JoF

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Fungi work as decomposers to break down organic carbon, deposit recalcitrant carbon, and transform other elements such as nitrogen. The decomposition of biomass is a key function of wood-decaying basidiomycetes and ascomycetes, which have the potential for the bioremediation of hazardous chemicals present in the environment. Due to their adaptation to different environments, fungal strains have a diverse set of phenotypic traits. This study evaluated 320 basidiomycetes isolates across 74 species for their rate and efficiency of degrading organic dye. We found that dye-decolorization capacity varies among and within species. Among the top rapid dye-decolorizing fungi isolates, we further performed genome-wide gene family analysis and investigated the genomic mechanism for their most capable dye-degradation capacity. Class II peroxidase and DyP-type peroxidase were enriched in the fast-decomposer genomes. Gene families including lignin decomposition genes, reduction-oxidation genes, hydrophobin, and secreted peptidases were expanded in the fast-decomposer species. This work provides new insights into persistent organic pollutant removal by fungal isolates at both phenotypic and genotypic levels.

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Fungal Enzymes: Degradation and Detoxification of Organic and Inorganic Pollutants

Cited by 12 publications

References 117 publications

Unravelling an Efficient O2/Reductant-Dependent Catalytic Route in Heme Peroxygenases for Sustainable Applications

Unravelling an Efficient O2/Reductant-Dependent Catalytic Route in Heme Peroxygenases for Sustainable Applications

The role and mechanisms of microbes in dichlorodiphenyltrichloroethane (DDT) and its residues bioremediation

Genomic Diversity and Phenotypic Variation in Fungal Decomposers Involved in Bioremediation of Persistent Organic Pollutants

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