Fungal endophytic communities of two wild Rosa varieties and the role of an endophytic Seimatosporium sp. in enhancing host plant powdery mildew resistance

Zhao, Yi; Ji, Xiuling; Shen, Ting; Tang, Wei‐Guo; Li, Shao-shi; Zhu, Zheng-qing; Kumar, Jitendra; Li, Haiyan

doi:10.1007/s11104-019-04404-3

Cited by 7 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditionally, cultivation dependent techniques have been employed in endophyte diversity studies (Petrini et al, 1982 ; Sun et al, 2011 ; Chowdhary and Kaushik, 2015 ; Teasdale et al, 2018 ). Such studies are important to specifically characterize the individual isolated endophytic fungi, including testing their potential for being as inoculants to improve plant growth and health, or screening for novel biologically active secondary metabolites (Ding et al, 2009 ; Tejesvi et al, 2011 ; Khan et al, 2016 ; Depetris et al, 2020 ; Zhao et al, 2020 ). Nevertheless, the isolation of endophytic fungi has been limited by traditional methodology due to our inability to grow many fungi in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China

Zheng

Qiao

et al. 2021

Front. Fungal Biol.

View full text Add to dashboard Cite

Aquatic ecosystems contain tremendous plant and microbial diversity. However, little is known about endophyte diversity in aquatic plants. In this study, we investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent high-throughput sequencing methods. A total of 1,689 fungal isolates belonging to three phyla and 154 genera were obtained from 15,373 plant tissue segments of 30 aquatic plant species. The most abundant endophytic fungi were those in ascomycete genera Aspergillus, Ceratophoma, Fusarium, Penicillium, Phoma and Plectosporium. No difference in fungal isolation rates was observed among tissues from roots, stems, and leaves. Twenty tissue samples from three most common plant species were further subjected to culture-independent meta-barcode sequencing. The sequence-based analyses revealed a total of 1,074 OTUs belonging to six fungal phyla and 194 genera. Among the three plants, Batrachium bungei harbored the highest number of OTUs. Besides, a total of 66 genera were detected by two methods. Both the culture-dependent and independent methods revealed that aquatic plants in southwest China have abundant endophytic fungal diversity. This study significantly expands our knowledge of the fungal community of aquatic plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China

Zheng

Qiao

et al. 2021

Front. Fungal Biol.

View full text Add to dashboard Cite

show abstract

“…proposed the "entropy" theory of agriculture: the lower the entropy, the more orderly interactions between root microorganisms and the stronger the plant's stress resistance. In a study of microorganisms on rose powdery mildew, Zhao et al (2020) found that resistant varieties showed resistance traits by recruiting beneficial microorganisms, such as M7SB 41 (Seimatosporium sp. ), to maintain microbial community functions.…”

Section: Discussionmentioning

confidence: 99%

Disease-resistant varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) inhibit Plasmodiophora brassicae infestation by stabilising root flora structure

Fang,

Han,

Yue

2024

Front. Plant Sci.

View full text Add to dashboard Cite

The application of disease-resistant varieties is the most cost-effective method for solving the problem of clubroot. “Shangpin,” a disease-resistant variety of Chinese cabbage with broad-spectrum immunity to Plasmodiophora brassicae (P. brassicae), was screened in a previous study. Based on 16S rRNA sequencing technology, we annotated the compositional differences between the rhizosphere, rhizoplane, and endosphere bacterial communities of “Shangpin” and “83-1” under P. brassicae stress. Alpha diversity analysis showed that the abundance of microorganisms in the root system of “83-1” changed more than that of “Shangpin” after P. brassicae infestation, and Beta diversity analysis indicated that Flavobacterium and Sphingomonas may mediate clubroot resistance, while Nitrospira, Nitrosospira, and Pseudomonas may mediate P. brassicae infestation among the bacteria in the Top 10 abundances. Microbial functional analyses showed that the root microorganisms of “83-1” were metabolically weakened after P. brassicae inoculation and were inhibited in competition with pathogenic bacteria. Conversely, the root microorganisms of “Shangpin” maintained the strength of their metabolic capacity, which took a favorable position in competition with the pathogen and inhibited the growth and development of the pathogen, thus showing resistance. Root secretions of “Shangpin” significantly inhibited the incidence and disease index of clubroot, which indicated that under clubroot stress, resistant varieties maintain root microbial diversity and microbial community functions through specific root exudates, enriching the genera Flavobacterium and Sphingomonas, thus showing resistance. The results of this study reveal the resistance mechanism of resistant varieties to clubroot and provide new insights into the prevention and control of clubroot in Chinese cabbage.

show abstract

“…M7SB41 is a culturable endophyte that was isolated from R. multiflora Thunb, and identified to be Seimatosporium sp. Our pot experiments have demonstrated that M7SB41 effectively reduced the severity of tobacco PM [ 24 ]. However, the mechanisms employed by M7SB41 in PM control are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Wild Rosa Endophyte M7SB41-Mediated Host Plant’s Powdery Mildew Resistance

Zhao¹,

Mao

Tang

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

Our previous studies indicated that endophyte M7SB41 (Seimatosporium sp.) can significantly enhance host plants powdery mildew (PM) resistance. To recover the mechanisms, differentially expressed genes (DEGs) were compared between E+ (endophte-inoculated) and E− (endophyte-free) plants by transcriptomics. A total of 4094, 1200 and 2319 DEGs between E+ and E− were identified at 0, 24, and 72 h after plants had been infected with PM pathogen Golovinomyces cichoracearum, respectively. Gene expression pattern analysis displayed a considerable difference and temporality in response to PM stress between the two groups. Transcriptional profiling analysis revealed that M7SB41 induced plant resistance to PM through Ca2+ signaling, salicylic acid (SA) signaling, and the phenylpropanoid biosynthesis pathway. In particular, we investigated the role and the timing of the SA and jasmonic acid (JA)-regulated defensive pathways. Both transcriptomes and pot experiments showed that SA-signaling may play a prominent role in PM resistance conferred by M7SB41. Additionally, the colonization of M7SB41 could effectively increase the activities and the expression of defense-related enzymes under PM pathogen stress. Meanwhile, our study revealed reliable candidate genes from TGA (TGACG motif-binding factor), WRKY, and pathogenesis-related genes related to M7SB41-mediate resistance. These findings offer a novel insight into the mechanisms of endophytes in activating plant defense responses.

show abstract

Fungal endophytic communities of two wild Rosa varieties and the role of an endophytic Seimatosporium sp. in enhancing host plant powdery mildew resistance

Cited by 7 publications

References 55 publications

Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China

Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China

Disease-resistant varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) inhibit Plasmodiophora brassicae infestation by stabilising root flora structure

Wild Rosa Endophyte M7SB41-Mediated Host Plant’s Powdery Mildew Resistance

Contact Info

Product

Resources

About