Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

Maia, Natália da Costa; Souza, Patrícia Nirlane da Costa; Godinho, Bárbara Temponi Vilarino; Moreira, Silvino Intra; Abreu, Lucas M.; Jank, Liana

doi:10.1590/rbz4720170183

Cited by 9 publications

(3 citation statements)

References 26 publications

(23 reference statements)

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“…Ascomycota and Basidiomycota were the two dominant phyla in the five samples. This phenomenon has also been shown by other researchers [25,26,27,28]. In the root of P. sinese collected from Yanan, Shannxi province, most of the sequencing tags (94%) belonged to phylum Basidiomycota and order Russulales, but the tags could not be annotated to the family level.…”

Section: Discussionsupporting

confidence: 58%

The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

et al. 2019

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Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Internal Transcribed Spacer (ITS) sequences based on primers ITS5-1737 and ITS2-2043R to investigate the endophytic fungi diversity of P. sinese roots at the maturity stage, as collected from four provinces (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China). The ITS sequences were processed using QIIME and R software. A total of 374,875 effective tags were obtained, and 708 operational taxonomic units (OTUs) were yielded with 97% identity in the five samples. Ascomycota and Basidiomycota were the two dominant phyla in the five samples, and the genera Khuskia and Heydenia were the most abundant in the FJ and XJ samples, respectively, while the most abundant tags in the other three samples could not be annotated at the genus level. In addition, our study revealed that the FJ sample possessed the highest OTU numbers (242) and the NS sample had the lowest (86). Moreover, only 22 OTUs were present in all samples simultaneously. The beta diversity analysis suggested a division of two endophytic fungi groups: the FJ sample from the south of China and the other four samples from north or northwest China. Correlation analysis between the environmental factors and endophytic fungi at the class level revealed that Sordariomycetes and Pucciniomycetes had extremely significant positive correlations with the total carbon, annual average precipitation, and annual average temperature, while Leotiomycetes showed an extremely significant negative correlation with quick acting potassium. The results revealed significant differences in the root endophytic fungi diversity of P. sinese in different provinces and might be useful for growth promotion and biomass production in the future.

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Section: Discussionsupporting

confidence: 58%

The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

et al. 2019

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“…In this study, we performed the experiments with three strains of Fusarium that were isolated during an earlier study by Maia et al (2018). Samples were collected from the asymptomatic plants of P. maximum (cv.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Of all species, Panicum maximum Jacq is most prominent owing to its role as a productive forage grass commonly found in Brazilian markets (Araújo et al 2013). Recently, endophytic fungi that naturally colonize and induce asymptomatic infection in the stalk of P. maximum were isolated from different locations in Brazil (Maia et al 2018). Fusarium strains isolated from P. maximum (cv.…”

Section: Introductionmentioning

confidence: 99%

Panicum maximum as a reservoir of a potential maize pathogen

et al. 2020

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Endophytic fungi are capable of infecting plants without inducing the development of any observable symptoms. Certain fungal species can also coexist as latent pathogens in their specific hosts. Several species of Fusarium have been reported to form an association with grasses as endophytes or latent pathogens, some of which are also mycotoxin producers. In this study, we aimed to address whether forage grasses could act as potential reservoirs of species that are pathogenic to maize (Zea mays). To this end, we performed morphological and phylogenetic analyses of the elongation factor 1-α (EF-1α) and the second largest subunit of RNA polymerase II (RPB2) genes and characterized three fungal strains isolated from Panicum maximum (cv. Mombaça) as a new phylogenetic species, Fusarium gigantea, within the Fusarium fujikuroi species complex. Moreover, the results of the pathogenicity test, such as the toothpick inoculation assay, revealed that these species caused the stalk rot disease in maize when maintained under greenhouse conditions. This finding highlights P. maximum as a potential reservoir of this pathogen and its role as a threat to maize cultivation.

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Endophytic Fungi Assures Tropical Forage Grass Growth by Water Stress Tolerances

et al. 2021

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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

Cited by 9 publications

References 26 publications

The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

Panicum maximum as a reservoir of a potential maize pathogen

Endophytic Fungi Assures Tropical Forage Grass Growth by Water Stress Tolerances

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