Fungal endophytes in seeds and needles of Pinus monticola

Ganley, Rebecca J.; Newcombe, George

doi:10.1016/j.mycres.2005.10.005

Cited by 191 publications

(129 citation statements)

References 34 publications

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“…Some endophytes are known to be tissue specific (de Abreu et al 2010;Fisher et al 1993;Ganley and Newcombe 2006). However, results of this study provided no evidence that the Botryosphaeriaceae sampled are specific to either leaves or woody tissue, although the frequency of occurrence of some species such as S. viticola varied on tissue types.…”

Section: Neofusicoccum Kwambonambiense Represents Another Example Of contrasting

confidence: 59%

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

et al. 2014

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The Botryosphaeriaceae represents an important and diverse family of latent fungal pathogens of woody plants. While some species appear to have wide host ranges, others are reported only from single hosts. It is, however, not clear whether apparently narrow host ranges reflect specificity or if this is an artifact of sampling. We address this question by sampling leaves and branches of native South African trees from four different families, including Acacia karroo (Fabaceae), Celtis africana (Cannabaceae), Searsia lancea (Anacardiaceae) and Gymnosporia buxifolia (Celastraceae). As part of this process, two new species of the Botryosphaeriaceae, namely Tiarosporella africana sp. nov. and Aplosporella javeedii sp. nov., emerged from sequence comparisons based on the ITS rDNA, TEF-1α, β-tubulin and LSU rDNA gene regions. An additional five known species were identified including Neofusicoccum parvum, N. kwambonambiense, Spencermartinsia viticola, Diplodia pseudoseriata and Botryosphaeria dothidea. Despite extensive sampling of the trees, some of these species were not isolated on many of the hosts as was expected. For example, B. dothidea, which is known to have a broad host range but was found only on A. karroo. This could have resulted from the fact that it is a rare species in the region. With the exception of S. lancea, which was infected by A. javeedii all the hosts were infected by more than one Botryosphaeriaceae species. Collectively, the results suggest that some intrinsic host factors, possibly combined with local environmental conditions, affect the distribution and co-infectivity of various hosts by the Botryosphaeriaceae. 2This would counteract the general ability of a species in the Botryosphaeriaceae to infect a broad range of plants. The combination of host and environmental factors might also explain why some Botryosphaeriaceae with apparently broad host ranges, are found on different suites of hosts in different areas of the world.

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Section: Neofusicoccum Kwambonambiense Represents Another Example Of contrasting

confidence: 59%

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

et al. 2014

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“…Beauveria bassiana has been reported as an endophyte in a variety of plants, including maize Lewis 1991, 1992a, b;Wagner and Lewis 2000), Theobroma gileri (Evans et al 2003), Carpinus caroliniana (Bills and Polishook 1991), in seeds and needles of Pinus monticola (Ganley and Newcombe 2005) in opium poppies (Quesada-Moraga et al 2006), in banana tissue cultures (Akello et al 2007), and in coffee (Posada et al 2007). Bing and Lewis (1991) demonstrated that B. bassiana is able to invade maize plants via the epidermis, thereafter persisting in the plant though the entire growing season and reducing tunneling by Ostrinia nubilalis (Lepidoptera: Pyralidae).…”

Section: Introductionmentioning

confidence: 99%

Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana

2009

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A study was conducted to determine the effect of inoculation method and plant growth medium on colonization of sorghum by an endophytic Beauveria bassiana. Colonization of leaves, stems, and roots by B. bassiana was assessed 20-days after application of the fungus. Although B. bassiana established as an endophyte in sorghum leaves, stems, and roots regardless of inoculation method (leaf, seed, or soil inoculation), plant growth medium (sterile soil, non-sterile soil, or vermiculite) apparently influenced colonization rates. Seed inoculation with conidia caused no stem or leaf colonization by the fungus in non-sterile soil but did result in substantial endophytic colonization in vermiculite and sterile soil. Leaf inoculation did not result in root colonization, regardless of plant growth medium. Endophytic colonization was greater in leaves and stems than roots. Endophytic colonization by B. bassiana had no adverse effects on the growth of sorghum plants. Leaf inoculation with a conidial suspension proved to be the best method to introduce B. bassiana into sorghum leaves for plants growing in either sterile or nonsterile soil. Further research should focus on the virulence of endophytic B. bassiana against sorghum stem borers.

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“…were observed to have pathogenicity on healthy Japanese black pine needles (Suto & Ougi, 1998;Seo et al, 2012;Yamamoto et al, 1964). On the other hand, these fungi have been reported to be endophytic fungi of conifers, including the Japanese black pine (Yoo & Eom, 2012;Qadri et al, 2014;Ganley et al, 2004;Ganley & Newcombe, 2006). Thus, these fungi, which are considered to be phytopathogenic fungi, can be considered endophytic fungi of Japanese black pine needles in the initial stage after infection and cause disease with some typical symptoms and reproductive structures such as stromata in the latter stages.…”

Section: Discussionmentioning

confidence: 99%

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>

Kihara¹,

Ueno²,

Arase³

2015

OJF

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A specific and sensitive polymerase chain reaction (PCR) assay based on the internal transcribed spacer (ITS) region of rDNA sequences was developed to detect endophytic and phytopathogenic fungi from needles of the Japanese black pine, Pinus thunbergii. Sequences of the ITS regions of Lophodermium conigenum, Lecanosticta acicola, Pestalotiopsis neglecta, Rhizosphaera kalkhoffii, and Septorioides pini-thunbergii were compared, and each specific primer pair for these species was designed. First, the designed primer pairs were tested for their specificity to detect each species. A PCR product was amplified only each combination of species and its specific primer pair, confirming the specificity of the designed primer pairs. These primer pairs were also tested on DNA extracted from the needles of P. thunbergii. The PCR products were amplified not only in needles with lesions but also in healthy needles without symptoms. Furthermore, several endophytic and phytopathogenic fungi could be simultaneously detected from the same region in a needle. The PCR-mediated detection method developed in this study will be a valuable tool for the detection of the endophytic and phytopathogenic fungi, not only as a rapid diagnostic tool for early detection but also for monitoring variations in both the quality and quantity of the endophytic and phytopathogenic fungi in needles in Japanese black pines.

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Fungal endophytes in seeds and needles of Pinus monticola

Cited by 191 publications

References 34 publications

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>

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Fungal endophytes in seeds and needles of Pinus monticola

Cited by 191 publications

References 34 publications

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

Botryosphaeriaceae species overlap on four unrelated, native South African hosts

Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana

PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, &lt;i&gt;Pinus thunbergii&lt;/i&gt;

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PCR-Mediated Detection of Endophytic and Phytopathogenic Fungi from Needles of the Japanese Black Pine, <i>Pinus thunbergii</i>