Fungal endophyte-derived Fritillaria unibracteata var. wabuensis: diversity, antioxidant capacities in vitro and relations to phenolic, flavonoid or saponin compounds

Pan, Feng; Su, Tian-Jiao; Cai, Shi-Mei; Wu, Wei

doi:10.1038/srep42008

Cited by 84 publications

(69 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antioxidants such as phenolic compounds inhibit the formation of free radicals and maintain the normal redox regulation of the human body [3]. Such facts encouraged the search for antioxidant and anticancer compounds from natural sources [4].…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Modifiers Induce Bioactive Phenolic Metabolites in the Marine-Derived Fungus Penicillium brevicompactum

et al. 2018

View full text Add to dashboard Cite

Fungi usually contain gene clusters that are silent or cryptic under normal laboratory culture conditions. These cryptic genes could be expressed for a wide variety of bioactive compounds. One of the recent approaches to induce production of such cryptic fungal metabolites is to use histone deacetylases (HDACs) inhibitors. In the present study, the cultures of the marine-derived fungus Penicillium brevicompactum treated with nicotinamide and sodium butyrate were found to produce a lot of phenolic compounds. Nicotinamide treatment resulted in the isolation and identification of nine compounds 1–9. Sodium butyrate also enhanced the productivity of anthranilic acid (10) and ergosterol peroxide (11). The antioxidant as well as the antiproliferative activities of each metabolite were determined. Syringic acid (4), sinapic acid (5), and acetosyringone (6) exhibited potent in vitro free radical scavenging, (IC50 20 to 30 µg/mL) and antiproliferative activities (IC50 1.14 to 1.71 µM) against HepG2 cancer cell line. Furthermore, a pharmacophore model of the active compounds was generated to build up a structure-activity relationship.

show abstract

Section: Introductionmentioning

confidence: 99%

Epigenetic Modifiers Induce Bioactive Phenolic Metabolites in the Marine-Derived Fungus Penicillium brevicompactum

et al. 2018

View full text Add to dashboard Cite

show abstract

“…A DPPH-radical scavenging assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH; Sigma-Aldrich) was carried out as previously described [22] with some modifications. The DPPH reagent was freshly prepared in Methanol (0.04 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

“…The assay was repeated three times. The percent scavenging ability for DPPH-radicals was calculated as described in Pan et al 2017 [22]. Additionally, a measure of half-maximal inhibitory concentration (IC 50 ) for 50% DPPH scavenging activity was carried out for standards and EA fraction of MUS1 by fitting a linear regression to DPPH scavenging curves respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The total phenolic content (TPC) and total flavonoid content (TFC) of the fungal crude extracts obtained using the solvents EA, CH, and HX, were determined as described [22]. However, the assay volumes were halved (total volume = 12.5 ml) and methanol was used to dissolve the standards i.e., gallic acid (Himedia), rutin (Himedia), and the crude extracts (1 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Gauchan

Vélëz

Acharya

et al. 2020

Preprint

View full text Add to dashboard Cite

24Endophytes are microbial colonizers that reside in plants by symbiotic association produces 25 several biological classes of natural products. The current study focuses on the isolation and 26 characterization of bioactive compounds produced by endophytic fungi isolated from the 27 Himalayan yew (Taxus wallichiana) collected from the Mustang district of Nepal. The plant 28 materials were collected from the Lower-Mustang region in the month of October 2016 and the 29 endophytic fungi were isolated on artificial media from inner tissues of bark and needles. 30 Antimicrobial and antioxidant activity, along with total phenolic-and flavonoid-content assays, 31 were used in the evaluation of bioactivity of the fermented crude extracts along with the in vitro 32 ability of the endophytes to produce the anticancer compound Taxol was analyzed. A total of 16 33 fungal morphotypes were obtained from asymptomatic inner tissues of the bark and needles of T. 34 wallichiana. Among the 16 isolates, the ethyl acetate (EA) fraction of isolate MUS1, showed 35 antibacterial and antifungal activity against all test-pathogens used, with significant inhibition 36 against Pseudomonas aeruginosa ATCC 27853 (MIC: 250 µg/ml) and the pathogenic yeast, 37 Candida albicans (MIC: 125 µg/ml). Antioxidant activity was also evaluated using 2,2-diphenyl-38 1-picrylhydrazyl (DPPH). At a concentration of 100 µg/ml, the % radical scavenging activity 39 was 83.15±0.40, 81.62±0.11, and 62.36±0.29, for ascorbic acid, butylated hydroxytoluene (BHT) 40 and the EA fraction of MUS1, respectively. The DPPH-IC50 value for the EA fraction was 81.52 41 µg/ml, compared to BHT (62.87 µg/ml) and ascorbic acid (56.15 µg/ml). The total phenolic and 42 flavonoid content in the EA fraction were 16.90±0.075 µg gallic acid equivalent (GAE) and 43 11.59±0.148 µg rutin equivalent (RE), per mg of dry crude extract, respectively. Isolate MUS1, 44 identified as an Annulohypoxylon sp. by ITS sequencing, also produced Taxol (282.05 µg/L) as 45 shown by TLC and HPLC analysis. Having the ability to produce antimicrobial and antioxidant 46 3 compounds, as well as the anticancer compound Taxol, makes Annulohypoxylon sp. strain 47 MUS1, a promising candidate for further study given that naturally occurring bioactive 48 compounds are of great interest to the pharmacological, food and cosmetic industries.49

show abstract

“…One of these fungal endophytes, Fusarium redolens 6WBY3 was capable of producing and secreting in the culture medium peimisine and imperialine-3β-D-glucoside whereas a second endophytic fungus was found to secrete peimisine and peiminine. Interestingly, a large number of the remaining endophytes were able to produce large amounts of antioxidants, such rosemarinic acid (Pan et al, 2014;Pan et al, 2015;Pan et al, 2017).…”

Section: Fritillaria Cirrhosa Ddon (Liliaceae)mentioning

confidence: 99%

Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts

Venieraki

Dimou

Katinakis

2017

Hellenic Plant Protection Journal

View full text Add to dashboard Cite

SummaryMedicinal plants have been used for thousands of years in folk medicines and still are used for their health benefi ts. In our days medicinal plants are exploited for the isolation of plant-derived drugs as they are very eff ective and have relatively less or no side eff ects. However, the natural resources of medicinal plants are gradually exhausted and access to plant bioactive compounds is challenged by the low levels at which these products accumulate in native medicinal plants. For instance, to meet the market demands of 3 Kg per year of vinca alkaloids, powerful plant-derived anticancer drugs, 1.5x106 Kg dry leaves are required. In this regard, this review aims to highlight the fact that endophytic fungi residing in medicinal plants are capable to biosynthesize pharmacologically active secondary metabolites similar or identical to those produced by their host medicinal plant. Furthermore, the evolutionary origin of the genes involved in these metabolic pathways as well as the approaches designed to enhance the production of these metabolites by the isolated endophytic fungi are also discussed.Additional key words: metabolites from endophytic bacteria and actinomycetes, chemical ecology present in virtually all organs of a given plant host, and some are seed borne. Endophytes often confer considerable benefi ts to the host plant they inhabit, since they can promote the growth of host plants, enhance resistance to biotic and abiotic stresses (Rodriguez et al., 2009; Hardoim et al., 2015), and accumulate bioactive secondary metabolites (Kusari et al., 2012). The ecological role of secondary metabolites produced by endophytes is not clear. However, recent studies have shown that these metabolites are involved in deterrence of herbivory (Pannaccione et al., 2014), protection against fungal (Soliman et al., 2015) or bacterial pathogens (Mousa et al., 2017) and amelioration of plant abiotic stress (Hamayum et al., 2016). Bioactive secondary metabolites derived from medicinal plants are gradually decreasing -Alternative approaches for their productionMedicinal plants, as a rich source of nat-

show abstract

Fungal endophyte-derived Fritillaria unibracteata var. wabuensis: diversity, antioxidant capacities in vitro and relations to phenolic, flavonoid or saponin compounds

Cited by 84 publications

References 56 publications

Epigenetic Modifiers Induce Bioactive Phenolic Metabolites in the Marine-Derived Fungus Penicillium brevicompactum

Epigenetic Modifiers Induce Bioactive Phenolic Metabolites in the Marine-Derived Fungus Penicillium brevicompactum

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts

Contact Info

Product

Resources

About