Fungal diversity present on rocks from a polar desert in continental Antarctica assessed using DNA metabarcoding

Menezes, Graciéle Cunha Alves de; Câmara, Paulo Eduardo Aguiar Saraiva; Pinto, Otávio Henrique Bezerra; Carvalho‐Silva, Micheline; Oliveira, Fábio Soares de; Souza, Caroline Delpupo; Schaefer, Carlos Ernesto Gonçalves Reynaud; Convey, Peter; Rosa, Carlos A.; Rosa, Luiz Henrique

doi:10.1007/s00792-021-01221-4

Cited by 20 publications

(17 citation statements)

References 62 publications

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“…In addition, recognized plant and animal pathogens were detected with different abundances, including Aspergillus , Candida , Cryptococcus , Cutaneotrichosporon , Fusarium , Malassezia and Rhodotorula . The same functional ecological profile detected for fungi present in marine sediments here have also been reported in studies sampling fungi in soil 12 , air 31 , lake sediment 43 and rocks 49 in Antarctica. However, we recognize that, as this study detected environmental DNA, further functional ecological studies are required to better understand the role and importance of fungi in these Antarctic marine sediments.…”

Section: Discussionsupporting

confidence: 87%

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Silva

Souza

Vieira

et al. 2022

Sci Rep

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We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications.

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Section: Discussionsupporting

confidence: 87%

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Silva

Souza

Vieira

et al. 2022

Sci Rep

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“…Considering the unique habitat provided by arid areas, it seems that the typical harsh environmental conditions of extreme desert lands truly reduce the number of plant endophytes. However, compared to other studies in similar extreme environments [26,73,74], the diversity of these microbial communities in our study is still relatively high in this arid desert, which may be associated with microenvironmental heterogeneity that is characteristic of the arid environment. Massimo et al [9] also demonstrated that fungal endophytes in desert plants were highly diverse and distinctive symbionts but infrequent in culture.…”

Section: Fungal Community Associated With Xerophytic Desert Shrubscontrasting

confidence: 80%

Fungal Endophytic Community and Diversity Associated with Desert Shrubs Driven by Plant Identity and Organ Differentiation in Extremely Arid Desert Ecosystem

Zuo

Xia

Yang

et al. 2021

JoF

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Despite desert ecosystem being crucial to our understanding of natural geography, species evolution and global climate change, there is limited information on the dynamics of their composition and the diversity of endophytic fungi communities driven by plant identity and organ differentiation. Here, an extensive investigation of endophytic fungal microbiome in root, stem, and leaf organs associated with five xerophyte shrubs in an extremely arid desert, Northwest China, were examined. The fungal community dominated by Dothideomycetes and Pleosporales. Shrub species strongly drive the niche-based processes of endophytic fungi across the root, stem and leaf compartments. The diversity and composition of endophytic fungi in stem showed higher variability among plant species than leaf and root. The fungal communities in root libraries were more diverse and exhibited a remarkable differentiation of community composition. We further demonstrated the significant host preferences and tissue specificity of desert endophytic fungi, and unique specific taxa were also observed. The co-occurrence network revealed the coexistence of fungal endophytes in arid desert, and the root fungal network harbored the highest interspecies connectivity. Members of Pleosporales were the most common keystone species in the root fungal network. This is the first report of mycobiota in both plant species and organ differentiation in an extremely arid desert ecosystem.

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“…Fungal Taxonomy, Fungal Diversity, and Fungal Lifestyle Existing studies of endolithic fungal communities present in Antarctic rocks have involved their direct observation in the substrate, cultivation techniques, or cultureindependent methods [13]. Rocks from Antarctic can be considered a unique microhabitat for life forms, providing a natural habitat for rock-inhabiting microbes, including fungal taxa [7,13,17]. In the extreme conditions across Antarctica, endolithic fungi present within rocks are dominated by the phyla Ascomycota and Basidiomycota, as revealed by studies using traditional culturing methods [13].…”

Section: Discussionmentioning

confidence: 99%

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

Rabelo,

Gonçalves,

Carvalho

et al. 2024

Biology

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In this study, we evaluated the fungal diversity present associated with cores of Oligocene rocks using a DNA metabarcoding approach. We detected 940,969 DNA reads grouped into 198 amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Mucoromycota, Rozellomycota, Blastocladiomycota, Monoblepharomycota, Zoopagomycota, Aphelidiomycota (Fungi) and the fungal-like Oomycota (Stramenopila), in rank abundance order. Pseudogymnoascus pannorum, Penicillium sp., Aspergillus sp., Cladosporium sp., Aspergillaceae sp. and Diaporthaceae sp. were assessed to be dominant taxa, with 22 fungal ASVs displaying intermediate abundance and 170 being minor components of the assigned fungal diversity. The data obtained displayed high diversity indices, while rarefaction indicated that the majority of the diversity was detected. However, the diversity indices varied between the cores analysed. The endolithic fungal community detected using a metabarcoding approach in the Oligocene rock samples examined contains a rich and complex mycobiome comprising taxa with different lifestyles, comparable with the diversity reported in recent studies of a range of Antarctic habitats. Due to the high fungal diversity detected, our results suggest the necessity of further research to develop strategies to isolate these fungi in culture for evolutionary, physiological, and biogeochemical studies, and to assess their potential role in biotechnological applications.

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Fungal diversity present on rocks from a polar desert in continental Antarctica assessed using DNA metabarcoding

Cited by 20 publications

References 62 publications

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Fungal Endophytic Community and Diversity Associated with Desert Shrubs Driven by Plant Identity and Organ Differentiation in Extremely Arid Desert Ecosystem

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

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