Fungal diversity in the coastal waters of King George Island (maritime Antarctica)

Garmendia, Gabriela; Alvarez, Angie; Villarreal, Romina; Martínez-Silveira, Adalgisa; Wisniewski, Michael; Vero, Silvana

doi:10.1007/s11274-021-03112-4

Cited by 8 publications

(6 citation statements)

References 75 publications

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“…For this, we combined the use of direct plating and an SMF pre-treatment with three culture media and two incubation temperatures. This methodology enabled the detection of 94 species, which represent a diverse fungal community that exceeds the number of species typically detected in culture-dependent studies in the marine environment [71][72][73][74]. The combination of both approaches improved our ability to detect fungi in marine sediments, including rare and novel species.…”

Section: Discussionmentioning

confidence: 99%

“…However, to the extent of our knowledge, this medium has never been used in diversity studies. In contrast, DRBC represents a typical medium for the isolation of marine fungi [11,13,73,74]. This medium enabled the isolation of another great portion of species and, most notably, the detection of six Talaromyces species that were not obtained with any other medium (Figure 4).…”

Section: Effect Of In Vitro Conditions For Isolation Of Marine Fungimentioning

confidence: 99%

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Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa

Guerra-Mateo,

Cano-Lira,

Fernández-Bravo

et al. 2024

JoF

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The Mediterranean Sea stands out as a hotspot of biodiversity, whose fungal composition remains underexplored. Marine sediments represent the most diverse substrate; however, the challenge of recovering fungi in culture hinders the precise identification of this diversity. Concentration techniques like skimmed milk flocculation (SMF) could represent a suitable solution. Here, we compare the effectiveness in recovering filamentous ascomycetes of direct plating and SMF in combination with three culture media and two incubation temperatures, and we describe the fungal diversity detected in marine sediments. Sediments were collected at different depths on two beaches (Miracle and Arrabassada) on the Spanish western Mediterranean coast between 2021 and 2022. We recovered 362 strains, and after a morphological selection, 188 were identified primarily with the LSU and ITS barcodes, representing 54 genera and 94 species. Aspergillus, Penicillium, and Scedosporium were the most common genera, with different percentages of abundance between both beaches. Arrabassada Beach was more heterogeneous, with 42 genera representing 60 species (Miracle Beach, 28 genera and 54 species). Although most species were recovered with direct plating (70 species), 20 species were exclusively obtained using SMF as a sample pre-treatment, improving our ability to detect fungi in culture. In addition, we propose three new species in the genera Exophiala, Nigrocephalum, and Queenslandipenidiella, and a fourth representing the novel genus Schizochlamydosporiella. We concluded that SMF is a useful technique that, in combination with direct plating, including different culture media and incubation temperatures, improves the chance of recovering marine fungal communities in culture-dependent studies.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of In Vitro Conditions For Isolation Of Marine Fungimentioning

confidence: 99%

Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa

Guerra-Mateo,

Cano-Lira,

Fernández-Bravo

et al. 2024

JoF

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“…Thus, its ability to survive in low pH and high concentrations of iron (II) sulfate heptahydrate in the S2 media enrichment, as observed in this study, implies that members of this genus are potentially acid-tolerant and further investigations may be required to determine their bioleaching potential. According to Amend [ 61 ], Malassezia are ecologically hyper-diverse and have been reported in diverse environmental sources, such as Antarctic soils [ 62 ], hydrothermal vents, and deep-sea sediments [ 61 ]. Spegazzenia is a wood saprotroph with no endophytic capability, as described in Table 4 , obtained from FungalTraits.…”

Section: Discussionmentioning

confidence: 99%

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Nkuna

Ijoma

Matambo

2022

JoF

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The hostile environment of mine tailings contains unique microbial life capable of bioleaching. The metagenomic analysis of such an environment provides an in-depth understanding of the microbial life and its potential, especially in biomining operations. However, DNA recovery from samples collected in those environments is challenging due to the presence of metal ions that interfere with the DNA analysis. A varied concentration of EDTA (4–13 µg/µL) to chelate the metal ions of enriched tailing samples prior to DNA extraction was performed. The results show that 9 µg/µL of EDTA was effective in most samples. However, the increasing concentration of EDTA negatively affected the DNA recovery. The sequencing of the successfully extracted DNA revealed a diverse range of fungal genera, some of which have not been previously reported in tailing or bioleaching applications. The dominant genera include Fodinomyces, Penicillium, Recurvomuces, Trichoderma, and Xenoacremonium; their traits were determined using the FungalTraits database. This study demonstrates the need to include a preliminary metal-chelating step using EDTA before DNA extractions for samples collected from metal-rich environments. It further showed the need for optimization but provided a benchmark range, particularly for tailings. However, we caution that a further EDTA removal step from the extracted DNA should be included to avoid its interferences in downstream applications.

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“…Despite the low numbers, the diversity is not low. For example, when studying the diversity of culturable yeasts in five locations in coastal waters on King George Island (maritime Antarctica), Garmendia et al [ 218 ] identified eleven genera. The analysis by massive sequencing of the region ITS2 showed great diversity since 31 yeast genera were found.…”

Section: Main Marine Organisms Containing Carotenoidsmentioning

confidence: 99%

Microalgae, Seaweeds and Aquatic Bacteria, Archaea, and Yeasts: Sources of Carotenoids with Potential Antioxidant and Anti-Inflammatory Health-Promoting Actions in the Sustainability Era

Mapelli-Brahm,

Gómez-Villegas,

Gonda

et al. 2023

Marine Drugs

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Carotenoids are a large group of health-promoting compounds used in many industrial sectors, such as foods, feeds, pharmaceuticals, cosmetics, nutraceuticals, and colorants. Considering the global population growth and environmental challenges, it is essential to find new sustainable sources of carotenoids beyond those obtained from agriculture. This review focuses on the potential use of marine archaea, bacteria, algae, and yeast as biological factories of carotenoids. A wide variety of carotenoids, including novel ones, were identified in these organisms. The role of carotenoids in marine organisms and their potential health-promoting actions have also been discussed. Marine organisms have a great capacity to synthesize a wide variety of carotenoids, which can be obtained in a renewable manner without depleting natural resources. Thus, it is concluded that they represent a key sustainable source of carotenoids that could help Europe achieve its Green Deal and Recovery Plan. Additionally, the lack of standards, clinical studies, and toxicity analysis reduces the use of marine organisms as sources of traditional and novel carotenoids. Therefore, further research on the processing of marine organisms, the biosynthetic pathways, extraction procedures, and examination of their content is needed to increase carotenoid productivity, document their safety, and decrease costs for their industrial implementation.

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Fungal diversity in the coastal waters of King George Island (maritime Antarctica)

Cited by 8 publications

References 75 publications

Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa

Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Microalgae, Seaweeds and Aquatic Bacteria, Archaea, and Yeasts: Sources of Carotenoids with Potential Antioxidant and Anti-Inflammatory Health-Promoting Actions in the Sustainability Era

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