Fungal Dermatitis Caused by Aspergillus sydowii in a Thoroughbred Horse

Lee, Sang Kyu; Kang, Hyun‐Gu; Na, Ki-Jeong; Han, Jae-Ik

doi:10.1016/j.jevs.2012.03.013

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…A few Aspergillus species are considered pathogenic to humans and animals. 3 The fungus Aspergillus sydowii is a mesophilic soil and marine saprophyte and it is a food contaminant and an opportunistic pathogen for humans. 4 Biotransformations of steroids by A. sydowii have not been described in the literature.…”

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The Biotransformation of Some Steroids by Aspergillus Sydowii MRC 200653

Yildirim

Kuru

2016

Journal of Chemical Research

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Fungal steroid biotransformations have been widely used for a long time in order to convert and synthesize steroids due to their remarkable regio-and stereo-selectivities. 1 Aspergillus species have been extensively studied in the context of mycotoxin production, pathogenicity, fundamental eukaryotic genetics and in biotechnology. 2 They are ubiquitous fungi found in soil, water and decaying materials. A few Aspergillus species are considered pathogenic to humans and animals. 3 The fungus Aspergillus sydowii is a mesophilic soil and marine saprophyte and it is a food contaminant and an opportunistic pathogen for humans. 4 Biotransformations of steroids by A. sydowii have not been described in the literature. In this work, testosterone 1, dehydroepiandrosterone 2, progesterone 3 and pregnenolone 4 were incubated with A. sydowii MRC 200653 for 5 days.Incubation of testosterone 1 with A. sydowii MRC 200653 for 5 days yielded three metabolites (Table 1). The first metabolite was identified as 6β,17β-dihydroxyandrost-4-en-3-one 5 (Fig. 1). The metabolite had new resonances at δ H 4.35 ppm (1H, bs) 5 and δ C 72.57 ppm 6 (Table 2), which were characteristic of a 6β-hydroxy group. The 1 H NMR spectrum of the metabolite showed a downfield shift (∆ 0.10 ppm) for the 4-H resonance of 1 at δ H 5.71 ppm (1H, s), supporting the presence of a 6β-hydroxy group. The 13 C NMR spectrum of the metabolite showed a downfield shift for C-7 (∆ 5.77 ppm), whereas it showed a γ-gauche upfield shift for C-8 (∆ 5.53 ppm), further supporting the presence of a 6β-hydroxy group.The second metabolite was identified as 14α,17βdihydroxyandrost-4-en-3-one 6. The 13 C NMR spectrum of the metabolite had a new carbon resonance at δ C 83.30 ppm, showing the presence of a hydroxy group. There was a significant downfield shift (∆ 0.70 ppm) for the 17α-H resonance suggesting a diaxial interaction with a 14α-hydroxy group. The 13 C NMR spectrum of the metabolite showed downfield shifts for C-8 (∆ 3.66 ppm) and C-15 (∆ 3.15 ppm) and there were γ-gauche upfield shifts for C-9 (∆ 6.78 ppm) and C-16 (∆ 0.12 ppm). These shifts revealed that a 14α-hydroxylation had taken place.

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The Biotransformation of Some Steroids by Aspergillus Sydowii MRC 200653

Yildirim

Kuru

2016

Journal of Chemical Research

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“…Aspergillus sydowii, another Aspergillus species determined in this study, has been known as saprobe in soil as well as pathogen to human (Chiu et al 2005), animal (Lee et al 2012), and corals (Greco et al 2017). Although many strains of A. sydowii causes disease in several groups of organisms, however, several strains of A. sydowii were reported to produce potential antimicrobial bioactive compounds such as a bisabolane-type sesquiterpenoids, namely aspergillusene D, two new xanthones, and two new catechol-derivatives, of which the xanthones displayed selective inhibitory activities against two influenza A virus (Liu et al 2019), and sydowiols A-C (1-3) that exhibited protein tyrosine phosphatase inhibitors activity of Mycobacterium tuberculosis which is important for the treatment of tuberculosis (Liu et al 2013).…”

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confidence: 91%

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana

Hidayat

Dewi

Sukmawati

2019

JMSB

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Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.

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“…cause a range of clinical syndromes in horses, including guttural pouch disease, pneumonia, and skin infections, with variable responses to treatment. [1][2][3][4][5]9,12,13,19 In guttural pouch mycosis in horses, A. fumigatus, A. nidulans, A. niger, and A. versicolor are frequently implicated. 4,5,13 Some horses with aspergillosis of the guttural pouch are reported to respond to topical itraconazole, but the curative treatment is to remove the blood supply by embolization of the internal carotid artery.…”

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Dermatitis, cellulitis, and osteomyelitis caused by Aspergillus nidulans in a horse with pituitary pars intermedia dysfunction

Waitt Wolker,

Black,

Lee

2024

J VET Diagn Invest

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Clinical and histologic examination of a 12-y-old client-owned Quarter Horse gelding with pituitary pars intermedia dysfunction revealed dermatitis, cellulitis, and osteomyelitis caused by Aspergillus nidulans, confirmed by a PCR assay. This novel presentation of a fungal disease in a horse was characterized by aggressive local invasion and failure to respond to all medical therapy attempted over a 1-y period. Treatments included systemic and topical antifungals, anti-inflammatories, and use of cellular matrices. Surgical excision was not attempted but should be strongly considered early in the disease process in similar cases if clean margins can be achieved. Postmortem findings were of locally aggressive disease with no dissemination.

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Fungal Dermatitis Caused by Aspergillus sydowii in a Thoroughbred Horse

Cited by 8 publications

References 25 publications

The Biotransformation of Some Steroids by Aspergillus Sydowii MRC 200653

The Biotransformation of Some Steroids by Aspergillus Sydowii MRC 200653

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana

Dermatitis, cellulitis, and osteomyelitis caused by Aspergillus nidulans in a horse with pituitary pars intermedia dysfunction

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